Role of and in Regulating Metamorphosis and Ovary Development in (Stål)

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Feb 15

PMID 35163119

Authors

Han Gao

Xiaojuan Jiang

Shiwen Zheng

Yan Li

Xinda Lin

Affiliations

Soon will be listed here.

Abstract

Juvenile hormone and ecdysone are key regulators in the metamorphosis and development. Grocho (Gro) is a highly conserved protein required for metamorphosis and development. Brown planthopper () is a major pest affecting rice production in China and many Asian countries. Although the molecular function of has been investigated in holometabolous insects such as and , their role in the hemimetabolous insect, brown planthopper, and the relationship between / and JH/ecdysone signaling pathway, remained unknown. In this study, () and () were cloned. An analysis of the predicted protein sequence showed that NlGro has highly conserved Q domain and WD40 domain, and NlGro1-L has a highly conserved WD40 domain. The expression profiles of both genes were studied by quantitative real-time PCR (qRT-PCR). Their relative expressions were high in egg, head, wing, ovary, and testis. and were found to interact genetically with juvenile hormone and ecdysone signaling by hormone treatment and RNAi of JH/ecdysone signaling-related genes. Moreover, when or was down-regulated alone, the survival rate was decreased, the ovarian development was delayed, and the oviposition was also affected. All defects were aggravated when and were down-regulated together. This study will help to develop new pesticides on the basis of the function of and , and provide new possibilities for the control of .

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