UPLC-PDA-MS/MS Profiling and Healing Activity of Polyphenol-Rich Fraction of Against Oral Ulcer in Rats

Overview

Journal Plants (Basel)

Date 2022 Feb 15

PMID 35161436

Authors

Hala El-Zahar

Esther T Menze

Heba Handoussa

Ahmed K Osman

Mohamed El-Shazly

Nada M Mostafa

Noha Swilam

Affiliations

Soon will be listed here.

Abstract

Camelthorn, Boiss, family Fabaceae has long been used in African folk medicine owing to its richness in pharmacologically active metabolites. The crude extract (CEAM), ethyl acetate fraction (EFAM) and -butanol (BFAM) fraction of aerial parts were investigated for their total polyphenols and oral antiulcer activity using in-vitro and in-vivo models. The major phenolic compound was isolated from the polyphenol-rich EFAM fraction and identified by conventional and spectroscopic methods of analysis as isorhamnetin-3--rutinoside. Furthermore, standardization of EAFM using UPLC-PDA-UV quantified isorhamnetin-3--rutinoside as 262.91 0.57 g/mg of the fraction. Analysis of EFAM using UPLC-PDA-MS/MS revealed tentative identification of 25 polyphenolic compounds. EFAM exhibited the most potent free radical scavenging activity against DPPH, with an IC (27.73 ± 1.85 µg/mL) and an FRAP value of (176.60 ± 5.21 μM Trolox equivalent (TE)/mg fraction) in comparison with CEAM and BFAM. Acetic acid-induced oral ulcers in a rat model were used to evaluate the healing properties of aerial parts. EFAM significantly decreased tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) by 36.4% and 50.8%, respectively, in the ulcer tissues while, CEAM and BFAM exhibited lower activity at the same dose. In addition, EFAM led to a significant ( < 0.0001) rise in the expression of proliferating cell nuclear antigen (PCNA), a cell proliferation marker. exhibited a potent healing effect in acetic acid-induced oral ulcers in rats by mitigating the release of pro-inflammatory cytokines and improving PCNA expression.

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