Stochastic Microsensors Based on Carbon Nanotubes for Molecular Recognition of the Isocitrate Dehydrogenases 1 and 2

Overview

Journal Nanomaterials (Basel)

Date 2022 Feb 15

PMID 35159804

Authors

Raluca-Ioana Stefan-van Staden

Catalina Cioates Negut

Sorin Sebastian Gheorghe

Paula Sfirloaga

Affiliations

Soon will be listed here.

Abstract

Two three-dimensional (3D) stochastic microsensors based on immobilization of protoporphyrin IX (PIX) in single-walled carbon nanotubes (SWCNT) and multi-walled carbon nanotubes (MWCNT) decorated with copper (Cu) and gold (Au) nanoparticles were designed and used for the molecular recognition of isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) in biological samples (brain tumor tissues, whole blood). The linear concentration ranges obtained for the molecular recognition and quantification of IDH1 and IDH2 were: IDH1 (1 × 10-1 × 10 ng mL) and IDH2 (5 × 10 - 5 × 10 ng mL). The limits of quantification obtained using the proposed microsensors were: 10 fg mL for IDH1 and 5 × 10 fg mL for IDH2. The highest sensitivities were obtained for the microsensor based on MWCNT. High recoveries versus enzyme-linked immunosorbent assay (ELISA) standard method were recorded for the assays of IDH1 and IDH2, all values being higher than 99.00%, with relative standard deviations (RSD) lower than 0.10%.

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