Pregnant Women and Endocrine Disruptors: Role of P2X7 Receptor and Mitochondrial Alterations in Placental Cell Disorders

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Feb 15

PMID 35159304

Authors

Sophie Fouyet

Elodie Olivier

Pascale Leproux

Melody Dutot

Patrice Rat

Affiliations

Soon will be listed here.

Abstract

In pregnant women, the lungs, skin and placenta are exposed daily to endocrine-disrupting chemicals (EDCs). EDCs induce multiple adverse effects, not only on endocrine organs, but also on non-endocrine organs, with the P2X7 cell death receptor being potentially the common key element. Our objective was first to investigate mechanisms of EDCs toxicity in both endocrine and non-endocrine cells through P2X7 receptor activation, and second, to compare the level of activation in lung, skin and placental cells. In addition, apoptosis in placental cells was studied because the placenta is the most exposed organ to EDCs and has essential endocrine functions. A total of nine EDCs were evaluated on three human cell models. We observed that the P2X7 receptor was not activated by EDCs in lung non-endocrine cells but was activated in skin and placenta cells, with the highest activation in placenta cells. P2X7 receptor activation and apoptosis are pathways shared by all tested EDCs in endocrine placental cells. P2X7 receptor activation along with apoptosis induction could be key elements in understanding endocrine placental and skin disorders induced by EDCs.

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References

Adinolfi E, Giuliani A, De Marchi E, Pegoraro A, Orioli E, Di Virgilio F . The P2X7 receptor: A main player in inflammation. Biochem Pharmacol. 2017; 151:234-244. DOI: 10.1016/j.bcp.2017.12.021. View

Etzel T, Calafat A, Ye X, Chen A, Lanphear B, Savitz D . Urinary triclosan concentrations during pregnancy and birth outcomes. Environ Res. 2017; 156:505-511. PMC: 5495558. DOI: 10.1016/j.envres.2017.04.015. View

Ferrari D, Los M, Bauer M, Vandenabeele P, Wesselborg S, Schulze-Osthoff K . P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death. FEBS Lett. 1999; 447(1):71-5. DOI: 10.1016/s0014-5793(99)00270-7. View

Makrantonaki E, Zouboulis C . Androgens and ageing of the skin. Curr Opin Endocrinol Diabetes Obes. 2009; 16(3):240-5. DOI: 10.1097/MED.0b013e32832b71dc. View

Urabe S, Miyoshi H, Fujiwara H, Yamaoka K, Kudo Y . Enhanced expression of P2X4 and P2X7 purinergic receptors in the myometrium of pregnant rats in preterm delivery models. Reprod Sci. 2009; 16(12):1186-92. DOI: 10.1177/1933719109344630. View

Leung D, Smith S, To K, Sahota D, Baker P . Increased placental apoptosis in pregnancies complicated by preeclampsia. Am J Obstet Gynecol. 2001; 184(6):1249-50. DOI: 10.1067/mob.2001.112906. View

Padmini E, Lavanya S, Uthra V . Preeclamptic placental stress and over expression of mitochondrial HSP70. Clin Chem Lab Med. 2009; 47(9):1073-80. DOI: 10.1515/CCLM.2009.247. View

Zhang Q, Wu S, Liu L, Hou X, Jiang J, Wei X . Effects of bisphenol A on gap junctions in HaCaT cells as mediated by the estrogen receptor pathway. J Appl Toxicol. 2018; 39(2):271-281. DOI: 10.1002/jat.3717. View

Fodor P, White B, Khan R . Inflammation-The role of ATP in pre-eclampsia. Microcirculation. 2019; 27(1):e12585. DOI: 10.1111/micc.12585. View

10.

Rat P, Olivier E, Tanter C, Wakx A, Dutot M . A fast and reproducible cell- and 96-well plate-based method for the evaluation of P2X7 receptor activation using YO-PRO-1 fluorescent dye. J Biol Methods. 2019; 4(1):e64. PMC: 6708926. DOI: 10.14440/jbm.2017.136. View

11.

Dutot M, Olivier E, Fouyet S, Magny R, Hammad K, Roulland E . In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects. Mar Drugs. 2021; 19(1). PMC: 7828825. DOI: 10.3390/md19010034. View

12.

Ribeiro D, Oliveira-Giacomelli A, Glaser T, Arnaud-Sampaio V, Andrejew R, Dieckmann L . Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology. Mol Psychiatry. 2020; 26(4):1044-1059. PMC: 7738776. DOI: 10.1038/s41380-020-00965-3. View

13.

Duprez L, Wirawan E, Vanden Berghe T, Vandenabeele P . Major cell death pathways at a glance. Microbes Infect. 2009; 11(13):1050-62. DOI: 10.1016/j.micinf.2009.08.013. View

14.

Paciencia I, Cavaleiro Rufo J, Silva D, Martins C, Mendes F, Farraia M . Exposure to indoor endocrine-disrupting chemicals and childhood asthma and obesity. Allergy. 2019; 74(7):1277-1291. DOI: 10.1111/all.13740. View

15.

Kong Q, Wang M, Liao Z, Camden J, Yu S, Simonyi A . P2X(7) nucleotide receptors mediate caspase-8/9/3-dependent apoptosis in rat primary cortical neurons. Purinergic Signal. 2008; 1(4):337-47. PMC: 2096553. DOI: 10.1007/s11302-005-7145-5. View

16.

Muller T, Vieira R, Grimm M, Durk T, Cicko S, Zeiser R . A potential role for P2X7R in allergic airway inflammation in mice and humans. Am J Respir Cell Mol Biol. 2010; 44(4):456-64. DOI: 10.1165/rcmb.2010-0129OC. View

17.

Ihde E, Zamudio S, Loh J, Zhu Y, Woytanowski J, Rosen L . Application of a novel mass spectrometric (MS) method to examine exposure to Bisphenol-A and common substitutes in a maternal fetal cohort. Hum Ecol Risk Assess. 2019; 24(2):331-346. PMC: 6777866. DOI: 10.1080/10807039.2017.1381831. View

18.

Silva G, Sperotto N, Borges T, Bonorino C, Takyia C, Coutinho-Silva R . P2X7 receptor is required for neutrophil accumulation in a mouse model of irritant contact dermatitis. Exp Dermatol. 2013; 22(3):184-8. DOI: 10.1111/exd.12094. View

19.

Travaglino A, Raffone A, Saccone G, Migliorini S, Maria Maruotti G, Esposito G . Placental morphology, apoptosis, angiogenesis and epithelial mechanisms in early-onset preeclampsia. Eur J Obstet Gynecol Reprod Biol. 2019; 234:200-206. DOI: 10.1016/j.ejogrb.2018.12.039. View

20.

Dai Q, Provost M, Raburn D, Price T . Progesterone Increases Mitochondria Membrane Potential in Non-human Primate Oocytes and Embryos. Reprod Sci. 2020; 27(5):1206-1214. DOI: 10.1007/s43032-019-00132-2. View