STIM1-dependent Peripheral Coupling Governs the Contractility of Vascular Smooth Muscle Cells

Overview

Journal Elife

Specialty Biology

Date 2022 Feb 11

PMID 35147077

Authors

Vivek Krishnan

Sher Ali

Albert L Gonzales

Pratish Thakore

Caoimhin S Griffin

Evan Yamasaki

Michael G Alvarado

Martin T Johnson

Mohamed Trebak

Scott Earley

Affiliations

Soon will be listed here.

Abstract

Peripheral coupling between the sarcoplasmic reticulum (SR) and plasma membrane (PM) forms signaling complexes that regulate the membrane potential and contractility of vascular smooth muscle cells (VSMCs). The mechanisms responsible for these membrane interactions are poorly understood. In many cells, STIM1 (stromal interaction molecule 1), a single-transmembrane-domain protein that resides in the endoplasmic reticulum (ER), transiently moves to ER-PM junctions in response to depletion of ER Ca stores and initiates store-operated Ca entry (SOCE). Fully differentiated VSMCs express STIM1 but exhibit only marginal SOCE activity. We hypothesized that STIM1 is constitutively active in contractile VSMCs and maintains peripheral coupling. In support of this concept, we found that the number and size of SR-PM interacting sites were decreased, and SR-dependent Ca-signaling processes were disrupted in freshly isolated cerebral artery SMCs from tamoxifen-inducible, SMC-specific STIM1-knockout (smKO) mice. VSMCs from smKO mice also exhibited a reduction in nanoscale colocalization between Ca-release sites on the SR and Ca-activated ion channels on the PM, accompanied by diminished channel activity. smKO mice were hypotensive, and resistance arteries isolated from them displayed blunted contractility. These data suggest that STIM1 - independent of SR Ca store depletion - is critically important for stable peripheral coupling in contractile VSMCs.

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