Valorization of Taiwan's Hayata Peels As a Source of Nobiletin and Tangeretin Using Simple Ultrasonic-assisted Extraction

Overview

Journal Curr Res Food Sci

Date 2022 Feb 11

PMID 35146444

Authors

Wei-Jyun Chien

Dinar S Saputri

Hung-Yu Lin

Affiliations

Soon will be listed here.

Abstract

As the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddHO) were used in the present study to extract flavonoids from Hayata peels with extraction period and fruit maturity as other variables. DPPH scavenging activity was investigated. Qualitative flavonoid content analysis was done by UV/Vis and FTIR-ATR spectra. Quantification of flavonoid using LC-MS/MS found that solvent type, fruit maturity, and ultrasonication period significantly affect the extracted flavonoid yield (p < 0.05). Extraction using 50% ethanol showed a higher yield than ddHO. Flavonoid content was also higher in unripe than ripe samples. Nobiletin, tangeretin, and rutin were dominant among the identified compounds in all sample treatments. Flavonoid content in Hayata extract was found to negatively correlate to DPPH scavenging activity, which needs further research to identify other bioactivities of these flavonoids.

Citing Articles

The Anticancer Perspective of Tangeretin: A Small Review.

Xu Y, Yan X, Zhuang J, Hao H Molecules. 2025; 30(2).

PMID: 39860170 PMC: 11767889. DOI: 10.3390/molecules30020300.

Anticancer, antimicrobial and antioxidant activity of CuO-ZnO bimetallic nanoparticles: green synthesised from Eryngium foetidum leaf extract.

Daimari J, Deka A Sci Rep. 2024; 14(1):19506.

PMID: 39174638 PMC: 11341821. DOI: 10.1038/s41598-024-69847-w.

Citrus depressa peel extract acts as a prebiotic to reduce lipid accumulation and modulate gut microbiota in obese mice.

Su Z, Liao J, Lin C, Wei G, Tung Y J Food Drug Anal. 2024; 32(2):213-226.

PMID: 38934690 PMC: 11210465. DOI: 10.38212/2224-6614.3504.

Effects of Hayata juice on high-fat diet-induced obesity in HBV transgenic mice.

Chu P, Hsu C, Lin Y, Pan Y, Dai Y, Yu Y Heliyon. 2024; 10(2):e24438.

PMID: 38312542 PMC: 10835261. DOI: 10.1016/j.heliyon.2024.e24438.

Fruit waste: a current perspective for the sustainable production of pharmacological, nutraceutical, and bioactive resources.

Sha S, Modak D, Sarkar S, Roy S, Sah S, Ghatani K Front Microbiol. 2023; 14:1260071.

PMID: 37942074 PMC: 10628478. DOI: 10.3389/fmicb.2023.1260071.

References

DuPont M, Day A, Bennett R, Mellon F, Kroon P . Absorption of kaempferol from endive, a source of kaempferol-3-glucuronide, in humans. Eur J Clin Nutr. 2004; 58(6):947-54. DOI: 10.1038/sj.ejcn.1601916. View

Kim D, Lim S . Extraction of flavanones from immature Citrus unshiu pomace: process optimization and antioxidant evaluation. Sci Rep. 2020; 10(1):19950. PMC: 7673033. DOI: 10.1038/s41598-020-76965-8. View

Chaves J, de Souza M, da Silva L, Lachos-Perez D, Torres-Mayanga P, da Fonseca Machado A . Extraction of Flavonoids From Natural Sources Using Modern Techniques. Front Chem. 2020; 8:507887. PMC: 7546908. DOI: 10.3389/fchem.2020.507887. View

Patle T, Shrivas K, Kurrey R, Upadhyay S, Jangde R, Chauhan R . Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV-vis and FTIR spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc. 2020; 242:118717. DOI: 10.1016/j.saa.2020.118717. View

Rupasinghe H, Kathirvel P, Huber G . Ultra-sonication-assisted solvent extraction of quercetin glycosides from 'Idared' apple peels. Molecules. 2011; 16(12):9783-91. PMC: 6264348. DOI: 10.3390/molecules16129783. View

Herrera-Pool E, Ramos-Diaz A, Lizardi-Jimenez M, Pech-Cohuo S, Ayora-Talavera T, Cuevas-Bernardino J . Effect of solvent polarity on the Ultrasound Assisted extraction and antioxidant activity of phenolic compounds from habanero pepper leaves (Capsicum chinense) and its identification by UPLC-PDA-ESI-MS/MS. Ultrason Sonochem. 2021; 76:105658. PMC: 8273200. DOI: 10.1016/j.ultsonch.2021.105658. View

Abarca-Vargas R, Pena Malacara C, Petricevich V . Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) Extracts. Antioxidants (Basel). 2016; 5(4). PMC: 5187543. DOI: 10.3390/antiox5040045. View

Stalikas C . Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci. 2007; 30(18):3268-95. DOI: 10.1002/jssc.200700261. View

Bakoyiannis I, Daskalopoulou A, Pergialiotis V, Perrea D . Phytochemicals and cognitive health: Are flavonoids doing the trick?. Biomed Pharmacother. 2018; 109:1488-1497. DOI: 10.1016/j.biopha.2018.10.086. View

10.

Formagio A, Volobuff C, Santiago M, Cardoso C, do Carmo Vieira M, Pereira Z . Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants (Basel). 2016; 3(4):745-57. PMC: 4665508. DOI: 10.3390/antiox3040745. View

11.

Stuetz W, Prapamontol T, Hongsibsong S, Biesalski H . Polymethoxylated flavones, flavanone glycosides, carotenoids, and antioxidants in different cultivation types of tangerines ( Citrus reticulata Blanco cv. Sainampueng) from Northern Thailand. J Agric Food Chem. 2010; 58(10):6069-74. DOI: 10.1021/jf904608h. View

12.

Uckoo R, Jayaprakasha G, Vikram A, Patil B . Polymethoxyflavones Isolated from the Peel of Miaray Mandarin (Citrus miaray) Have Biofilm Inhibitory Activity in Vibrio harveyi. J Agric Food Chem. 2015; 63(32):7180-9. DOI: 10.1021/acs.jafc.5b02445. View

13.

Asikin Y, Taira I, Inafuku-Teramoto S, Sumi H, Ohta H, Takara K . The composition of volatile aroma components, flavanones, and polymethoxylated flavones in Shiikuwasha (Citrus depressa Hayata) peels of different cultivation lines. J Agric Food Chem. 2012; 60(32):7973-80. DOI: 10.1021/jf301848s. View

14.

Ganeshpurkar A, Saluja A . The Pharmacological Potential of Rutin. Saudi Pharm J. 2017; 25(2):149-164. PMC: 5355559. DOI: 10.1016/j.jsps.2016.04.025. View

15.

Bouderias S, Teszlak P, Jakab G, Korosi L . Age- and season-dependent pattern of flavonol glycosides in Cabernet Sauvignon grapevine leaves. Sci Rep. 2020; 10(1):14241. PMC: 7455724. DOI: 10.1038/s41598-020-70706-7. View

16.

Shiu Y, Lin H, Chi C, Yeh S, Liu C . Effects of hirami lemon, Citrus depressa Hayata, leaf meal in diets on the immune response and disease resistance of juvenile barramundi, Lates calcarifer (bloch), against Aeromonas hydrophila. Fish Shellfish Immunol. 2016; 55:332-8. DOI: 10.1016/j.fsi.2016.06.001. View

17.

Catauro M, Papale F, Bollino F, Piccolella S, Marciano S, Nocera P . Silica/quercetin sol-gel hybrids as antioxidant dental implant materials. Sci Technol Adv Mater. 2016; 16(3):035001. PMC: 5099839. DOI: 10.1088/1468-6996/16/3/035001. View

18.

Wang X, Zhou B, Wu D, Chen X, Li B, Wang L . Ultrasound-based one-step fabrication of nobiletin particle: A facile stabilization strategy. Food Chem. 2021; 369:130896. DOI: 10.1016/j.foodchem.2021.130896. View

19.

Baranowska I, Bajkacz S . A new UHPLC-MS/MS method for the determination of flavonoids in supplements and DPPH-UHPLC-UV method for the evaluation of the radical scavenging activity of flavonoids. Food Chem. 2018; 256:333-341. DOI: 10.1016/j.foodchem.2018.02.138. View

20.

Enogieru A, Haylett W, Hiss D, Bardien S, Ekpo O . Rutin as a Potent Antioxidant: Implications for Neurodegenerative Disorders. Oxid Med Cell Longev. 2018; 2018:6241017. PMC: 6040293. DOI: 10.1155/2018/6241017. View