Enhancement of Proliferation of Human Umbilical Cord Blood-derived CD34 Hematopoietic Stem Cells by a Combination of Hyper-interleukin-6 and Small Molecules

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2022 Feb 11

PMID 35146134

Authors

Yo Seph Park

Yukyeong Lee

Na Young Choi

Han Sung Hwang

Stefan Rose-John

Martin Zenke

Kinarm Ko

Affiliations

Soon will be listed here.

Abstract

Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation to the use of UCB-derived HSCs (UCB-HSCs) in transplantation, however, is the low numbers of HSCs in a unit of cord blood. To overcome this limitation, various cytokines or small molecules have been used to expand UCB-HSCs ex vivo. In this study, we investigated a synergistic effect of the combination of HIL-6, SR1, and UM171 on UCB-HSC culture and found that this combination resulted in the highest number of CD34 cells. These results suggest that the combination of SR1, UM171 and HIL-6 exerts a synergistic effect in the proliferation of HSCs from UCB and thus, SR1, UM171 and HIL-6 is the most suitable combination for obtaining HSCs from UCB for clinical transplantation.

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