Physiological and Metabolic Effects of Healthy Female Aging on Exhaled Breath Biomarkers

Overview

Journal iScience

Publisher Cell Press

Date 2022 Feb 10

PMID 35141500

Authors

Pritam Sukul

Simon Grzegorzewski

Celine Broderius

Phillip Trefz

Thomas Mittlmeier

Dagmar-Christiane Fischer

Wolfram Miekisch

Jochen K Schubert

Affiliations

Soon will be listed here.

Abstract

Healthy aging driven physio-metabolic events in females hold the key to complex mechanistic links and systemic cross talks. Effects from basic changes at genome, proteome, metabolome, and lipidome levels are often reflected at the upstream phenome (e.g., breath volatome) cascades. Here, we have analyzed exhaled volatile metabolites (measured via real time mass spectrometry based breathomics) data from 204 healthy females, aged between 07 and 80 years. Age related substance-specific differences were observed in breath biomarkers. Exhalation of blood-borne endogenous organosulfur, short-chain fatty acids, alcohols, aldehydes, alkene, ketones and exogenous nitriles, terpenes, and aromatics have denominated interplay between endocrine differences, energy homeostasis, systemic microbial diversity, oxidative stress, and lifestyle. Overall marker expressions were suppressed under daily oral contraception. Young homosexual/lesbian adults turned out as breathomic outliers. Previously proposed disease-specific breath biomarkers should be reevaluated upon aging effects. Breathomics offers a noninvasive window toward system-wide understanding and personalized monitoring of aging i.e., translatable to gerontology.

Citing Articles

Exhaled breath metabolites reveal postmenopausal gut-bone cross-talk and non-invasive markers for osteoporosis.

Sukul P, Fischer D, Broderius C, Grzegorzewski S, Rahn A, Mittlmeier T Commun Med (Lond). 2024; 4(1):279.

PMID: 39732987 PMC: 11682404. DOI: 10.1038/s43856-024-00723-4.

Effects of Contagious Respiratory Pathogens on Breath Biomarkers.

Kemnitz N, Fuchs P, Remy R, Ruehrmund L, Bartels J, Klemenz A Antioxidants (Basel). 2024; 13(2).

PMID: 38397770 PMC: 10886173. DOI: 10.3390/antiox13020172.

Origin of breath isoprene in humans is revealed via multi-omic investigations.

Sukul P, Richter A, Junghanss C, Schubert J, Miekisch W Commun Biol. 2023; 6(1):999.

PMID: 37777700 PMC: 10542801. DOI: 10.1038/s42003-023-05384-y.

Measuring healthy ageing: current and future tools.

Silva N, Rajado A, Esteves F, Brito D, Apolonio J, Roberto V Biogerontology. 2023; 24(6):845-866.

PMID: 37439885 PMC: 10615962. DOI: 10.1007/s10522-023-10041-2.

Physio-metabolic and clinical consequences of wearing face masks-Systematic review with meta-analysis and comprehensive evaluation.

Kisielinski K, Hirsch O, Wagner S, Wojtasik B, Funken S, Klosterhalfen B Front Public Health. 2023; 11:1125150.

PMID: 37089476 PMC: 10116418. DOI: 10.3389/fpubh.2023.1125150.

References

Winpenny E, van Sluijs E, White M, Klepp K, Wold B, Lien N . Changes in diet through adolescence and early adulthood: longitudinal trajectories and association with key life transitions. Int J Behav Nutr Phys Act. 2018; 15(1):86. PMC: 6131755. DOI: 10.1186/s12966-018-0719-8. View

Chaudry A, Travers P, Yuenger J, Colletta L, Evans P, Zenilman J . Analysis of vaginal acetic acid in patients undergoing treatment for bacterial vaginosis. J Clin Microbiol. 2004; 42(11):5170-5. PMC: 525245. DOI: 10.1128/JCM.42.11.5170-5175.2004. View

Zhu Y, Liu X, Ding X, Wang F, Geng X . Telomere and its role in the aging pathways: telomere shortening, cell senescence and mitochondria dysfunction. Biogerontology. 2018; 20(1):1-16. DOI: 10.1007/s10522-018-9769-1. View

Poli D, Goldoni M, Corradi M, Acampa O, Carbognani P, Internullo E . Determination of aldehydes in exhaled breath of patients with lung cancer by means of on-fiber-derivatisation SPME-GC/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2010; 878(27):2643-51. DOI: 10.1016/j.jchromb.2010.01.022. View

Somani Y, Pawelczyk J, De Souza M, Kris-Etherton P, Proctor D . Aging women and their endothelium: probing the relative role of estrogen on vasodilator function. Am J Physiol Heart Circ Physiol. 2019; 317(2):H395-H404. PMC: 6732482. DOI: 10.1152/ajpheart.00430.2018. View

Boskey E, Telsch K, Whaley K, Moench T, Cone R . Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification. Infect Immun. 1999; 67(10):5170-5. PMC: 96867. DOI: 10.1128/IAI.67.10.5170-5175.1999. View

Zhang X, Zhong H, Li Y, Shi Z, Ren H, Zhang Z . Sex- and age-related trajectories of the adult human gut microbiota shared across populations of different ethnicities. Nat Aging. 2023; 1(1):87-100. DOI: 10.1038/s43587-020-00014-2. View

Whittemore K, Vera E, Martinez-Nevado E, Sanpera C, Blasco M . Telomere shortening rate predicts species life span. Proc Natl Acad Sci U S A. 2019; 116(30):15122-15127. PMC: 6660761. DOI: 10.1073/pnas.1902452116. View

Zeiner A, Kegg P . Effects of sex steroids on ethanol pharmacokinetics and autonomic reactivity. Prog Biochem Pharmacol. 1981; 18:130-42. View

10.

Corradi M, Folesani G, Andreoli R, Manini P, Bodini A, Piacentini G . Aldehydes and glutathione in exhaled breath condensate of children with asthma exacerbation. Am J Respir Crit Care Med. 2002; 167(3):395-9. DOI: 10.1164/rccm.200206-507OC. View

11.

Clarke G, Stilling R, Kennedy P, Stanton C, Cryan J, Dinan T . Minireview: Gut microbiota: the neglected endocrine organ. Mol Endocrinol. 2014; 28(8):1221-38. PMC: 5414803. DOI: 10.1210/me.2014-1108. View

12.

Zhu X, Gu H, Ni X . Hydrogen sulfide in the endocrine and reproductive systems. Expert Rev Clin Pharmacol. 2011; 4(1):75-82. DOI: 10.1586/ecp.10.125. View

13.

Tangerman A . Measurement and biological significance of the volatile sulfur compounds hydrogen sulfide, methanethiol and dimethyl sulfide in various biological matrices. J Chromatogr B Analyt Technol Biomed Life Sci. 2009; 877(28):3366-77. DOI: 10.1016/j.jchromb.2009.05.026. View

14.

Wilmanski T, Diener C, Rappaport N, Patwardhan S, Wiedrick J, Lapidus J . Gut microbiome pattern reflects healthy ageing and predicts survival in humans. Nat Metab. 2021; 3(2):274-286. PMC: 8169080. DOI: 10.1038/s42255-021-00348-0. View

15.

Sukul P, Oertel P, Kamysek S, Trefz P . Oral or nasal breathing? Real-time effects of switching sampling route onto exhaled VOC concentrations. J Breath Res. 2017; 11(2):027101. DOI: 10.1088/1752-7163/aa6368. View

16.

DeJong E, Surette M, Bowdish D . The Gut Microbiota and Unhealthy Aging: Disentangling Cause from Consequence. Cell Host Microbe. 2020; 28(2):180-189. DOI: 10.1016/j.chom.2020.07.013. View

17.

Bartoli M, Novelli F, Costa F, Malagrino L, Melosini L, Bacci E . Malondialdehyde in exhaled breath condensate as a marker of oxidative stress in different pulmonary diseases. Mediators Inflamm. 2011; 2011:891752. PMC: 3136125. DOI: 10.1155/2011/891752. View

18.

Elahi D, Muller D . Carbohydrate metabolism in the elderly. Eur J Clin Nutr. 2000; 54 Suppl 3:S112-20. DOI: 10.1038/sj.ejcn.1601032. View

19.

Kwa M, Plottel C, Blaser M, Adams S . The Intestinal Microbiome and Estrogen Receptor-Positive Female Breast Cancer. J Natl Cancer Inst. 2016; 108(8). PMC: 5017946. DOI: 10.1093/jnci/djw029. View

20.

Refat M, Moore T, Kazui M, Risby T, Perman J, Schwarz K . Utility of breath ethane as a noninvasive biomarker of vitamin E status in children. Pediatr Res. 1991; 30(5):396-403. DOI: 10.1203/00006450-199111000-00002. View