Effects of Repeated Ovarian Stimulation on Ovarian Function and Aging in Mice

Overview

Journal Dev Reprod

Specialty Biology

Date 2022 Feb 10

PMID 35141447

Authors

Jihye Whang

Cheyoung Ahn

Soohyun Kim

Eunji Seok

Yunjeong Yang

Goeun Han

Haeun Jo

Hyunwon Yang

Affiliations

Soon will be listed here.

Abstract

Controlled ovarian hyperstimulation (COH) is routinely used in the fertilization and embryo transfer (IVF-ET) cycles to increase the number of retrieved mature oocytes. However, the relationship between repeated COH and ovarian function is still controversial. Therefore, we investigated whether repeated ovarian stimulation affects ovarian aging and function, including follicular development, autophagy, and apoptosis in follicles. Ovarian hyperstimulation in mice was induced by intraperitoneal injection with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Mice subjected to ovarian stimulation once were used as a control group and 10 times as an experimental group. Repeated injections with PMSG and hCG significantly reduced the number of primary follicles compared to a single injection. The number of secondary and antral follicles increased slightly, while the number of corpus luteum increased significantly with repeated injections. On the other hand, repeated injections did not affect apoptosis in follicles associated with follicular atresia. The expression of autophagy-related genes , , , and , cell proliferation-related genes , apoptosis-related genes , and was not significantly different between the two groups. In addition, the expression of the aging-related genes , , and were also not significantly different. In this study, we demonstrated that repeated ovarian stimulation in mice affects follicular development, but not autophagy, apoptosis, aging in ovary. These results suggest that repetition of COH in the IVF-ET cycle may not result in ovarian aging, such as a decrease in ovarian reserve in adult women.

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