Butyrate in Combination with Forskolin Alleviates Necrotic Enteritis, Increases Feed Efficiency, and Improves Carcass Composition of Broilers

Overview

Journal J Anim Sci Biotechnol

Publisher Biomed Central

Date 2022 Feb 10

PMID 35139922

Authors

Qing Yang

Binlong Chen

Kelsy Robinson

Thiago Belem

Wentao Lyu

Zhuo Deng

Ranjith Ramanathan

Guolong Zhang

Affiliations

Soon will be listed here.

Abstract

Background: The emergence of antimicrobial resistance has necessitated the development of effective alternatives to antibiotics for livestock and poultry production. This study investigated a possible synergy between butyrate and forskolin (a natural labdane diterpene) in enhancing innate host defense, barrier function, disease resistance, growth performance, and meat quality of broilers.

Methods: The expressions of representative genes involved in host defense (AvBD9 and AvBD10), barrier function (MUC2, CLDN1, and TJP1), and inflammation (IL-1β) were measured in chicken HD11 macrophages in response to butyrate and forskolin in the presence or absence of bacterial lipopolysaccharides (LPS). Intestinal lesions and the Clostridium perfringens titers were also assessed in C. perfringens-challenged chickens fed butyrate and forskolin-containing Coleus forskohlii (CF) extract individually or in combination. Furthermore, growth performance and carcass characteristics were evaluated in broilers supplemented with butyrate and the CF extract for 42 d.

Results: Butyrate and forskolin synergistically induced the expressions of AvBD9, AvBD10, and MUC2 in chicken HD11 cells (P < 0.05) and the synergy was maintained in the presence of LPS. Butyrate and forskolin also suppressed LPS-induced IL-1β gene expression in HD11 cells in a synergistic manner (P < 0.05). The two compounds significantly reduced the intestinal lesions of C. perfringens-challenged chickens when combined (P < 0.05), but not individually. Furthermore, butyrate in combination with forskolin-containing CF extract had no influence on weight gain, but significantly reduced feed intake (P < 0.05) with a strong tendency to improve feed efficiency (P = 0.07) in a 42-d feeding trial. Desirably, the butyrate/forskolin combination significantly decreased abdominal fat deposition (P = 0.01) with no impact on the carcass yield, breast meat color, drip loss, or pH of d-42 broilers.

Conclusions: Butyrate and forskolin has potential to be developed as novel antibiotic alternatives to improve disease resistance, feed efficiency, and carcass composition of broilers.

Citing Articles

Butyric acid and prospects for creation of new medicines based on its derivatives: a literature review.

Gerunova L, Gerunov T, Pyanova L, Lavrenov A, Sedanova A, Delyagina M J Vet Sci. 2024; 25(2):e23.

PMID: 38568825 PMC: 10990906. DOI: 10.4142/jvs.23230.

Two intestinal microbiota-derived metabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis.

Kim D, Liu J, Whitmore M, Tobin I, Zhao Z, Zhang G J Anim Sci Biotechnol. 2024; 15(1):29.

PMID: 38429856 PMC: 10908072. DOI: 10.1186/s40104-024-00995-9.

Effects of infection doses on growth performance and gut health in dual-infection model of necrotic enteritis in broiler chickens.

Goo D, Choi J, Ko H, Choppa V, Liu G, Lillehoj H Front Physiol. 2023; 14:1269398.

PMID: 37799512 PMC: 10547889. DOI: 10.3389/fphys.2023.1269398.

Impact of buffered sodium butyrate as a partial or total dietary alternative to lincomycin on performance, IGF-1 and TLR4 genes expression, serum indices, intestinal histomorphometry, Clostridia, and litter hygiene of broiler chickens.

Bawish B, Zahran M, Ismael E, Kamel S, Ahmed Y, Hamza D Acta Vet Scand. 2023; 65(1):44.

PMID: 37770986 PMC: 10540366. DOI: 10.1186/s13028-023-00704-y.

Combined effect of microbially derived cecal SCFA and host genetics on feed efficiency in broiler chickens.

He Z, Liu R, Wang M, Wang Q, Zheng J, Ding J Microbiome. 2023; 11(1):198.

PMID: 37653442 PMC: 10472625. DOI: 10.1186/s40168-023-01627-6.

References

Peterson L, Artis D . Intestinal epithelial cells: regulators of barrier function and immune homeostasis. Nat Rev Immunol. 2014; 14(3):141-53. DOI: 10.1038/nri3608. View

Wu S, Rodgers N, Choct M . Real-time PCR assay for Clostridium perfringens in broiler chickens in a challenge model of necrotic enteritis. Appl Environ Microbiol. 2010; 77(3):1135-9. PMC: 3028694. DOI: 10.1128/AEM.01803-10. View

Chen Y, Wen J, Feng J, Wang Y, Li T, Nurmi K . Forskolin attenuates the NLRP3 inflammasome activation and IL-1β secretion in human macrophages. Pediatr Res. 2019; 86(6):692-698. DOI: 10.1038/s41390-019-0418-4. View

Bedford A, Yu H, Hernandez M, Squires E, Leeson S, Gong J . Effects of fatty acid glyceride product SILOhealth 104 on the growth performance and carcass composition of broiler chickens. Poult Sci. 2018; 97(4):1315-1323. DOI: 10.3382/ps/pex440. View

Mahlapuu M, Bjorn C, Ekblom J . Antimicrobial peptides as therapeutic agents: opportunities and challenges. Crit Rev Biotechnol. 2020; 40(7):978-992. DOI: 10.1080/07388551.2020.1796576. View

Shojadoost B, Vince A, Prescott J . The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens: a critical review. Vet Res. 2012; 43:74. PMC: 3546943. DOI: 10.1186/1297-9716-43-74. View

Biagi G, Piva A, Moschini M, Vezzali E, Roth F . Performance, intestinal microflora, and wall morphology of weanling pigs fed sodium butyrate. J Anim Sci. 2007; 85(5):1184-91. DOI: 10.2527/jas.2006-378. View

Chen J, Peng S, Cheng Y, Lee I, Yu Y . Effect of Forskolin on Body Weight, Glucose Metabolism and Adipocyte Size of Diet-Induced Obesity in Mice. Animals (Basel). 2021; 11(3). PMC: 8000574. DOI: 10.3390/ani11030645. View

Alasbahi R, Melzig M . Forskolin and derivatives as tools for studying the role of cAMP. Pharmazie. 2012; 67(1):5-13. View

10.

Jeukendrup A, Randell R . Fat burners: nutrition supplements that increase fat metabolism. Obes Rev. 2011; 12(10):841-51. DOI: 10.1111/j.1467-789X.2011.00908.x. View

11.

Chelakkot C, Ghim J, Ryu S . Mechanisms regulating intestinal barrier integrity and its pathological implications. Exp Mol Med. 2018; 50(8):1-9. PMC: 6095905. DOI: 10.1038/s12276-018-0126-x. View

12.

Bedford A, Yu H, Squires E, Leeson S, Gong J . Effects of supplementation level and feeding schedule of butyrate glycerides on the growth performance and carcass composition of broiler chickens. Poult Sci. 2017; 96(9):3221-3228. DOI: 10.3382/ps/pex098. View

13.

Zasloff M . Antimicrobial peptides of multicellular organisms. Nature. 2002; 415(6870):389-95. DOI: 10.1038/415389a. View

14.

Henderson S, Magu B, Rasmussen C, Lancaster S, Kerksick C, Smith P . Effects of coleus forskohlii supplementation on body composition and hematological profiles in mildly overweight women. J Int Soc Sports Nutr. 2008; 2:54-62. PMC: 2129145. DOI: 10.1186/1550-2783-2-2-54. View

15.

Godard M, Johnson B, Richmond S . Body composition and hormonal adaptations associated with forskolin consumption in overweight and obese men. Obes Res. 2005; 13(8):1335-43. DOI: 10.1038/oby.2005.162. View

16.

Zhang G, Sunkara L . Avian antimicrobial host defense peptides: from biology to therapeutic applications. Pharmaceuticals (Basel). 2014; 7(3):220-47. PMC: 3978490. DOI: 10.3390/ph7030220. View

17.

Zhang W, Gao F, Zhu Q, Li C, Jiang Y, Dai S . Dietary sodium butyrate alleviates the oxidative stress induced by corticosterone exposure and improves meat quality in broiler chickens. Poult Sci. 2011; 90(11):2592-9. DOI: 10.3382/ps.2011-01446. View

18.

Du X, Shi R, Wang Y, Wu W, Sun S, Dai Z . Isoforskolin and forskolin attenuate lipopolysaccharide-induced inflammation through TLR4/MyD88/NF-κB cascades in human mononuclear leukocytes. Phytother Res. 2019; 33(3):602-609. PMC: 6590664. DOI: 10.1002/ptr.6248. View

19.

Liu H, Wang J, He T, Becker S, Zhang G, Li D . Butyrate: A Double-Edged Sword for Health?. Adv Nutr. 2018; 9(1):21-29. PMC: 6333934. DOI: 10.1093/advances/nmx009. View

20.

Sunkara L, Zeng X, Curtis A, Zhang G . Cyclic AMP synergizes with butyrate in promoting β-defensin 9 expression in chickens. Mol Immunol. 2013; 57(2):171-80. DOI: 10.1016/j.molimm.2013.09.003. View