Immunohistochemical Localization of Neurofilaments and Neuron-specific Enolase in 29 Cases of Neuroblastoma

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1986 Mar 1

PMID 3513600

Citations 12

Authors

M Osborn

T Dirk

H Kaser

K Weber

M Altmannsberger

Affiliations

Soon will be listed here.

Abstract

Twenty-nine neuroblastomas have been examined with the use of rabbit antibodies specific for each of the three neurofilament polypeptides, with a monoclonal antibody specific for the NF-L polypeptide, and with a rabbit antibody specific for neuron-specific enolase. When frozen material was used, all neuroblastomas were positive with the neurofilaments antibodies. When alcohol-fixed paraffin-embedded material was used, neurofilament staining was weaker and the fixation procedure appeared to destroy the epitopes recognized by the NF-L antibodies preferentially. Although all neuroblastomas were positive for neurone-specific enolase, so were two rhabdomyosarcomas, suggesting that NSE is not an appropriate marker to distinguish the different small blue cell tumors of children.

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