Close Genetic Linkage Between Human and Companion Animal Extraintestinal Pathogenic ST127

Overview

Journal Curr Res Microb Sci

Specialty Microbiology

Date 2022 Feb 7

PMID 35128493

Authors

Paarthiphan Elankumaran

Glenn F Browning

Marc S Marenda

Cameron J Reid

Steven P Djordjevic

Affiliations

Soon will be listed here.

Abstract

ST127, a recently emerged global pathogen noted for high virulence gene carriage, is a leading cause of urinary tract and blood stream infections. ST127 is frequently isolated from humans and companion animals; however, it is unclear if they are distinct or related populations of ST127. We performed a phylogenomic analysis of 299 ST127 of diverse epidemiological origin to characterize their population structure, genetic determinants of virulence, antimicrobial resistance, and repertoire of mobile genetic elements with a focus on plasmids. The core gene phylogeny was divided into 13 clusters, the largest of which (BAP4) contained the majority of human and companion animal origin isolates. This dominant cluster displayed genetic differences to the remainder of the phylogeny, most notably alternative gene alleles encoding important virulence factors including lipid A, flagella, and K capsule. Furthermore, numerous close genetic linkages (<30 SNPs) between human and companion animal isolates were observed within the cluster. Carriage of antimicrobial resistance genes in the collection was limited, but virulence gene carriage was extensive. We found evidence of pUTI89-like virulence plasmid carriage in over a third of isolates, localised to four of the major phylogenetic clusters. Our study supports global scale repetitive transfer of ST127 lineages between humans and companion animals, particularly within the dominant BAP4 cluster.

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