Genetic Diversity of Colistin Resistance Nosocomial Strains from Iran

Overview

Journal J Res Med Sci

Specialty General Medicine

Date 2022 Feb 7

PMID 35126588

Authors

Maryam Seyyedi

Reza Shapouri

Habib Zeighami

Leili Shokoohizadeh

Affiliations

Soon will be listed here.

Abstract

Background: Drug-resistant is a global health problem since its ability to acquire new resistance mechanisms. Here, we aimed to determine the association of common types of and assess their drug resistance of and contribution of integrons () and oxacillinase genes in Zanjan, Iran.

Materials And Methods: Among 68 isolated Acinetobacters from patients, 48 isolates were strains. Antibiotic susceptibility pattern and colistin resistance were determined by disk diffusion and broth microdilution, respectively. The presence of , , , and oxacillinase genes examined using polymerase chain reaction. The clonal relationship of clinical isolates of determined by Pulsed Field Gel Electrophoresis method.

Results: The results showed the highest antibiotic susceptibility (58%) for colistin. 96% of isolates were considered as multidrug resistant, and 46% as extensively drug resistant, and 16% as pandrug resistant. Frequencies of , , resistance genes were 60%, 28%, and 0%, respectively, and 12% of strains had no isoform of Ints. Frequencies of Carbapenem resistance genes were 74%, 24%, 100%, and 4% for blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58, respectively. The above samples were group into 26 pulsotypes.

Conclusion: The studied strains had several resistance genes, and the colistin resistance showed an extraordinary ascending tendency that could be a severe issue in nosocomial infections, and the presence of high genetic diversity indicated a variation in strains and possibly a variety of sources of contamination or infection.

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