Relationship Between MiR-204 and ANGPTL2 Expression and Diagnosis, Pathological Stage, and Prognosis in Patients with Colon Cancer

Overview

Journal Transl Cancer Res

Specialty Oncology

Date 2022 Feb 4

PMID 35116678

Authors

Chenliang Wang

Rongfei Tan

Lizi Peng

Jing Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Angiopoietin-like protein 2 () is linked to various tumors. MicroRNA- (miR-204) is associated with colorectal cancer (CRC). Bioinformatic analysis has demonstrated a targeting relationship between miR-204 and . The present study aimed to investigate the role of miR-204 in the proliferation and apoptosis of colorectal tumor cells.

Methods: Colorectal tumor tissues were collected. Normal colon mucosa was used as a control. The relationship between miR-204 and expression and tumor stage and prognosis was analyzed. The dual-luciferase reporter assay confirmed targeted regulation between miR-204 and . SW480 cells were allocated to the miR-NC group and the miR-204 mimic group, followed by apoptotic analysis using flow cytometry and cellular proliferation analysis using EdU staining.

Results: Compared with normal colonic mucosa, miR-204 expression was decreased in colorectal tumor tissues and expression was increased, which correlated with TNM staging. The prognosis of patients with low miR-204 expression and high expression was worse than for patients with high miR-204 expression and low expression. The dual-luciferase reporter assay confirmed a targeting regulation relationship between miR-204 and . Transfection of miR-204 mimic significantly inhibited the expression of and cell proliferation in SW480 cells and promoted apoptosis.

Conclusions: Downregulating miR-204 expression plays a vital role in upregulating expression and promoting the pathogenesis of CRC. MiR-204 is able to hinder the proliferation of colorectal tumor cells and encourage apoptosis by targeting the inhibition of expression.

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