Analysis of β-agonists in Different Biological Matrices By Liquid Chromatography-tandem Mass Spectrometry

Overview

Journal J Vet Res

Publisher Sciendo

Specialty Veterinary Medicine

Date 2022 Feb 3

PMID 35112001

Authors

Tomasz Sniegocki

Bartosz Sell

Andrzej Posyniak

Affiliations

Soon will be listed here.

Abstract

Introduction: Wide use is made of β-agonists in therapy due to their smooth muscle-relaxant properties. They also have a side effect of increasing muscle mass. Besides improving oxygen utilisation as bronchodilators, β-agonists increase protein synthesis and promote fat burning. The growth- and performance-enhancing effects are often exploited in illegal use. The guiding objective of this study was to develop a procedure for the determination of β-agonists by a single method in different types of matrices.

Material And Methods: Five grams of homogenised samples were subjected to enzymatic hydrolysis with β-glucuronidase in ammonium acetate, pH 5.2. Purification was performed by solid phase extraction. Analytes were eluted with 10% acetic acid in methanol. The eluted β-agonists were analysed by high-performance liquid chromatography-tandem mass spectrometry.

Results: Validation results met the requirement of the confirmation criteria according to European Commission Decision 2002/657/EC in terms of apparent recoveries (93.2-112.0%), repeatability (3.1-7.1%) and intra-laboratory reproducibility (4.1-8.2%).

Conclusion: The method can be successfully applied in the detection and determination of clenbuterol, salbutamol, mabuterol, mapenterol, terbutaline, brombuterol, zilpaterol, isoxsuprine and ractopamine in feed, drinking water, urine, muscle, lung and liver matrices.

Citing Articles

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PMID: 38525223 PMC: 10960330. DOI: 10.2478/jvetres-2024-0001.

Chromium Methionine and Ractopamine Supplementation in Summer Diets for Grower-Finisher Pigs Reared under Heat Stress.

Sanchez-Villalba E, Corral-March E, Valenzuela-Melendres M, Zamorano-Garcia L, Celaya-Michel H, Ochoa-Meza A Animals (Basel). 2023; 13(16).

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References

Leyssens L, Driessen C, Jacobs A, Czech J, Raus J . Determination of beta 2-receptor agonists in bovine urine and liver by gas chromatography-tandem mass spectrometry. J Chromatogr. 1991; 564(2):515-27. DOI: 10.1016/0378-4347(91)80520-m. View

Beucher L, Dervilly-Pinel G, Prevost S, Monteau F, Le Bizec B . Determination of a large set of β-adrenergic agonists in animal matrices based on ion mobility and mass separations. Anal Chem. 2015; 87(18):9234-42. DOI: 10.1021/acs.analchem.5b01831. View

dos Ramos F . Beta2-agonist extraction procedures for chromatographic analysis. J Chromatogr A. 2000; 880(1-2):69-83. DOI: 10.1016/s0021-9673(00)00240-5. View

Sniegocki T, Sell B, Giergiel M, Posyniak A . QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices. Molecules. 2019; 24(3). PMC: 6384814. DOI: 10.3390/molecules24030384. View

Yikilmaz Y, Kuzukiran O, Erdogan E, Sen F, Kirmizibayrak O, Filazi A . The determination of β-agonist residues in bovine tissues using liquid chromatography-tandem mass spectrometry. Biomed Chromatogr. 2020; 34(10):e4926. DOI: 10.1002/bmc.4926. View

Guo P, Wan J, Zhan C, Zhu C, Jiang W, Ke Y . A simplified sample pretreatment for the rapid determination of 22 β-agonist residues in swine muscle and liver tissues by ultra-high-performance liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2018; 1096:122-134. DOI: 10.1016/j.jchromb.2018.07.038. View

Lee H, Sarafin K, Peart T . Determination of beta-blockers and beta2-agonists in sewage by solid-phase extraction and liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2007; 1148(2):158-67. DOI: 10.1016/j.chroma.2007.03.024. View

Decheng S, Peilong W, Yang L, Ruiguo W, Xia F, Xiaoou S . Evaluation of β-agonists in blood meal: Validation of determination method and potential pathway for reentry into the environment. J Chromatogr A. 2019; 1612:460624. DOI: 10.1016/j.chroma.2019.460624. View

Boyd D, OKeeffe M, Smyth M . Matrix solid-phase dispersion as a multiresidue extraction technique for beta-agonists in bovine liver tissue. Analyst. 1994; 119(7):1467-70. DOI: 10.1039/an9941901467. View

10.

Zhu Y, Xie S, Chen D, Pan Y, Qu W, Wang X . Targeted analysis and determination of β-agonists, hormones, glucocorticoid and psychiatric drugs in feed by liquid chromatography with electrospray ionization tandem mass spectrometry. J Sep Sci. 2016; 39(13):2584-94. DOI: 10.1002/jssc.201600011. View

11.

Dumasia M, Houghton E . Screening and confirmatory analysis of beta-agonists, beta-antagonists and their metabolites in horse urine by capillary gas chromatography-mass spectrometry. J Chromatogr. 1991; 564(2):503-13. DOI: 10.1016/0378-4347(91)80519-i. View

12.

Gressler V, Feddern V, Peixoto J, Ledur M, Dalla Costa O, de Lima G . Application of Enzyme Digestion and Deconjugation Followed by Quick, Easy, Cheap, Effective, Rugged, Safe Extraction and Liquid Chromatography-Tandem Mass Spectrometry Methodology To Determine Ractopamine Residue in Pork. J Food Prot. 2018; 81(8):1258-1263. DOI: 10.4315/0362-028X.JFP-17-526. View

13.

Liu H, Gan N, Chen Y, Ding Q, Huang J, Lin S . Novel method for the rapid and specific extraction of multiple β2 -agonist residues in food by tailor-made Monolith-MIPs extraction disks and detection by gas chromatography with mass spectrometry. J Sep Sci. 2016; 39(18):3578-85. DOI: 10.1002/jssc.201600479. View

14.

Cai J, Henion J . Quantitative multi-residue determination of beta-agonists in bovine urine using on-line immunoaffinity extraction-coupled column packed capillary liquid chromatography-tandem mass spectrometry. J Chromatogr B Biomed Sci Appl. 1997; 691(2):357-70. DOI: 10.1016/s0378-4347(96)00433-1. View

15.

Tang J, Wang J, Shi S, Hu S, Yuan L . Determination of -Agonist Residues in Animal-Derived Food by a Liquid Chromatography-Tandem Mass Spectrometric Method Combined with Molecularly Imprinted Stir Bar Sorptive Extraction. J Anal Methods Chem. 2018; 2018:9053561. PMC: 6036788. DOI: 10.1155/2018/9053561. View

16.

Wang P, Liu X, Su X, Zhu R . Sensitive detection of β-agonists in pork tissue with novel molecularly imprinted polymer extraction followed liquid chromatography coupled tandem mass spectrometry detection. Food Chem. 2015; 184:72-9. DOI: 10.1016/j.foodchem.2015.03.073. View

17.

Liu H, Lin X, Lin T, Zhang Y, Luo Y, Li Q . Magnetic molecularly imprinted polymers for the determination of β-agonist residues in milk by ultra high performance liquid chromatography with tandem mass spectrometry. J Sep Sci. 2016; 39(18):3594-601. DOI: 10.1002/jssc.201600613. View

18.

Hooijerink H, Schilt R, van Bennekom E, Huf F . Determination of beta-sympathomimetics in liver and urine by immunoaffinity chromatography and gas chromatography-mass-selective detection. J Chromatogr B Biomed Appl. 1994; 660(2):303-13. DOI: 10.1016/s0378-4347(94)80016-2. View

19.

Sniegocki T, Giergiel M, Sell B, Posyniak A . New Method of Analysis of Nitrofurans and Nitrofuran Metabolites in Different Biological Matrices Using UHPLC-MS/MS. J Vet Res. 2018; 62(2):161-166. PMC: 6200284. DOI: 10.2478/jvetres-2018-0025. View