Multi-omics Analyses Revealed Key Factors Involved in Fluorescent Carbon-dots-regulated Secondary Metabolism in Tetrastigma Hemsleyanum

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2022 Feb 3

PMID 35109871

Authors

Xin Peng

Zhuomi Xie

Xiuhua Wang

Yuxiang Zhao

Chuyun Yang

Zhongyi Zhang

Mingjie Li

Jianping Zheng

Yuhui Wang

Affiliations

Soon will be listed here.

Abstract

Background: Luminescent nanomaterials (LNMs), especially newly-exploited fluorescent carbon-dots (CDs), have demonstrated promising candidates for sunlight harvesting and enhanced photosynthesis efficiency of crops. However, most of the studies focus on the design and synthesis of LNMs and primary metabolism in biomass acceleration, secondary metabolism that closely associated with the quality ingredients of plants is rarely mentioned.

Results: UV-absorptive and water-soluble NIR-CDs were harvested via a facile microwave-assisted carbonization method. The effect and regulatory mechanism of NIR-CDs on the secondary metabolism and bioactive ingredients accumulation in Tetrastigma hemsleyanum were explored. A total of 191 differential secondary metabolites and 6874 differentially expressed genes were identified when the NIR-CDs were adopted for enhancing growth of T. hemsleyanum. The phenolic acids were generally improved, but the flavonoids were more likely to decrease. The pivotal differentially expressed genes were involved in biosynthesis of secondary metabolites, flavonoid biosynthesis, porphyrin and chlorophyll metabolism, etc. The gene-metabolite association was constructed and 44 hub genes highly related to quality ingredients accumulation and growth were identified, among which and the top 5 genes of the PPI network might be the key regulators.

Conclusion: This research provided key regulatory genes and differentially accumulating quality ingredients under NIR-CDs-treatment, which could provide a theoretical basis for expanding the applications of nanomaterial in industrial crop agriculture.

Citing Articles

Widely Targeted Metabolomic Analysis Revealed the Diversity in Milk from Goats, Sheep, Cows, and Buffaloes and Its Association with Flavor Profiles.

Zhang F, Wang Y, Liu B, Gong P, Shi C, Zhu L Foods. 2024; 13(9).

PMID: 38731736 PMC: 11083174. DOI: 10.3390/foods13091365.

References

Lowry G, Avellan A, Gilbertson L . Opportunities and challenges for nanotechnology in the agri-tech revolution. Nat Nanotechnol. 2019; 14(6):517-522. DOI: 10.1038/s41565-019-0461-7. View

Bojdys M, Muller J, Antonietti M, Thomas A . Ionothermal synthesis of crystalline, condensed, graphitic carbon nitride. Chemistry. 2008; 14(27):8177-82. DOI: 10.1002/chem.200800190. View

Giraldo J, Landry M, Faltermeier S, McNicholas T, Iverson N, Boghossian A . Plant nanobionics approach to augment photosynthesis and biochemical sensing. Nat Mater. 2014; 13(4):400-8. DOI: 10.1038/nmat3890. View

Raliya R, Nair R, Chavalmane S, Wang W, Biswas P . Mechanistic evaluation of translocation and physiological impact of titanium dioxide and zinc oxide nanoparticles on the tomato (Solanum lycopersicum L.) plant. Metallomics. 2015; 7(12):1584-94. DOI: 10.1039/c5mt00168d. View

Ji T, Ji W, Wang J, Chen H, Peng X, Cheng K . A comprehensive review on traditional uses, chemical compositions, pharmacology properties and toxicology of Tetrastigma hemsleyanum. J Ethnopharmacol. 2020; 264:113247. PMC: 7422820. DOI: 10.1016/j.jep.2020.113247. View

Pazdernik M, Mares J, Pilny J, Sobotka R . The antenna-like domain of the cyanobacterial ferrochelatase can bind chlorophyll and carotenoids in an energy-dissipative configuration. J Biol Chem. 2019; 294(29):11131-11143. PMC: 6643027. DOI: 10.1074/jbc.RA119.008434. View

Jaganathan D, Ramasamy K, Sellamuthu G, Jayabalan S, Venkataraman G . CRISPR for Crop Improvement: An Update Review. Front Plant Sci. 2018; 9:985. PMC: 6056666. DOI: 10.3389/fpls.2018.00985. View

Buchfink B, Xie C, Huson D . Fast and sensitive protein alignment using DIAMOND. Nat Methods. 2014; 12(1):59-60. DOI: 10.1038/nmeth.3176. View

Langfelder P, Horvath S . WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics. 2008; 9:559. PMC: 2631488. DOI: 10.1186/1471-2105-9-559. View

10.

Zhang M, Hu L, Wang H, Song Y, Liu Y, Li H . One-step hydrothermal synthesis of chiral carbon dots and their effects on mung bean plant growth. Nanoscale. 2018; 10(26):12734-12742. DOI: 10.1039/c8nr01644e. View

11.

Qian S, Qiao L, Xu W, Jiang K, Wang Y, Lin H . An inner filter effect-based near-infrared probe for the ultrasensitive detection of tetracyclines and quinolones. Talanta. 2019; 194:598-603. DOI: 10.1016/j.talanta.2018.10.097. View

12.

Peng X, Wu H, Chen H, Zhang Y, Qiu D, Zhang Z . Transcriptome profiling reveals candidate flavonol-related genes of Tetrastigma hemsleyanum under cold stress. BMC Genomics. 2019; 20(1):687. PMC: 6717372. DOI: 10.1186/s12864-019-6045-y. View

13.

Ding H, Wei J, Zhang P, Zhou Z, Gao Q, Xiong H . Solvent-Controlled Synthesis of Highly Luminescent Carbon Dots with a Wide Color Gamut and Narrowed Emission Peak Widths. Small. 2018; 14(22):e1800612. DOI: 10.1002/smll.201800612. View

14.

Jiang D, Li P, Yin Y, Ren G, Liu C . Molecular cloning and functional characterization of UGTs from Glycyrrhiza uralensis flavonoid pathway. Int J Biol Macromol. 2021; 192:1108-1116. DOI: 10.1016/j.ijbiomac.2021.09.136. View

15.

Travlou N, Algarra M, Alcoholado C, Cifuentes-Rueda M, Labella A, Lazaro-Martinez J . Carbon Quantum Dot Surface-Chemistry-Dependent Ag Release Governs the High Antibacterial Activity of Ag-Metal-Organic Framework Composites. ACS Appl Bio Mater. 2022; 1(3):693-707. DOI: 10.1021/acsabm.8b00166. View

16.

Pan L, Sun S, Zhang L, Jiang K, Lin H . Near-infrared emissive carbon dots for two-photon fluorescence bioimaging. Nanoscale. 2016; 8(39):17350-17356. DOI: 10.1039/c6nr05878g. View

17.

Lou T, Ji T, Peng X, Ji W, Yuan L, Wang J . Extract From Leaf Alleviates Lung Infection: Network Pharmacology Analysis and Experimental Evidence. Front Pharmacol. 2021; 12:587850. PMC: 8326761. DOI: 10.3389/fphar.2021.587850. View

18.

Li D, Li W, Zhang H, Zhang X, Zhuang J, Liu Y . Far-Red Carbon Dots as Efficient Light-Harvesting Agents for Enhanced Photosynthesis. ACS Appl Mater Interfaces. 2020; 12(18):21009-21019. DOI: 10.1021/acsami.9b21576. View

19.

Colasante C, Zheng F, Kemp C, Voncken F . A plant-like mitochondrial carrier family protein facilitates mitochondrial transport of di- and tricarboxylates in Trypanosoma brucei. Mol Biochem Parasitol. 2018; 221:36-51. DOI: 10.1016/j.molbiopara.2018.03.003. View

20.

Nimi N, Saraswathy A, Nazeer S, Francis N, Shenoy S, Jayasree R . Multifunctional hybrid nanoconstruct of zerovalent iron and carbon dots for magnetic resonance angiography and optical imaging: An In vivo study. Biomaterials. 2018; 171:46-56. DOI: 10.1016/j.biomaterials.2018.04.012. View