Immunoaffinity Monoliths for Multiplexed Extraction of Preterm Birth Biomarkers from Human Blood Serum in 3D Printed Microfluidic Devices

Overview

Journal Analyst

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Feb 1

PMID 35103723

Authors

Haifa M Almughamsi

Makella K Howell

Samuel R Parry

Joule E Esene

Jacob B Nielsen

Gregory P Nordin

Adam T Woolley

Affiliations

Soon will be listed here.

Abstract

In an effort to develop biomarker-based diagnostics for preterm birth (PTB) risk, we created 3D printed microfluidic devices with multiplexed immunoaffinity monoliths to selectively extract multiple PTB biomarkers. The equilibrium dissociation constant for each monoclonal antibody toward its target PTB biomarker was determined. We confirmed the covalent attachment of three different individual antibodies to affinity monoliths using fluorescence imaging. Three different PTB biomarkers were successfully extracted from human blood serum using their respective single-antibody columns. Selective binding of each antibody toward its target biomarker was observed. Finally, we extracted and eluted three PTB biomarkers from depleted human blood serum in multiplexed immunoaffinity columns in 3D printed microfluidic devices. This is the first demonstration of multiplexed immunoaffinity extraction of PTB biomarkers in 3D printed microfluidic devices.

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References

Yang W, Sun X, Wang H, Woolley A . Integrated microfluidic device for serum biomarker quantitation using either standard addition or a calibration curve. Anal Chem. 2009; 81(19):8230-5. PMC: 2802465. DOI: 10.1021/ac901566s. View

Dincer C, Bruch R, Kling A, Dittrich P, Urban G . Multiplexed Point-of-Care Testing - xPOCT. Trends Biotechnol. 2017; 35(8):728-742. PMC: 5538621. DOI: 10.1016/j.tibtech.2017.03.013. View

Hill P . The measurement of albumin in serum and plasma. Ann Clin Biochem. 1985; 22 ( Pt 6):565-78. DOI: 10.1177/000456328502200604. View

Nielsen J, Hanson R, Almughamsi H, Pang C, Fish T, Woolley A . Microfluidics: Innovations in Materials and Their Fabrication and Functionalization. Anal Chem. 2019; 92(1):150-168. PMC: 7034066. DOI: 10.1021/acs.analchem.9b04986. View

Beauchamp M, Nielsen A, Gong H, Nordin G, Woolley A . 3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers. Anal Chem. 2019; 91(11):7418-7425. PMC: 6561337. DOI: 10.1021/acs.analchem.9b01395. View

Souza R, McKenzie E, Jones B, de Seymour J, Thomas M, Zarate E . Trace biomarkers associated with spontaneous preterm birth from the maternal serum metabolome of asymptomatic nulliparous women - parallel case-control studies from the SCOPE cohort. Sci Rep. 2019; 9(1):13701. PMC: 6757051. DOI: 10.1038/s41598-019-50252-7. View

Mori A, Saito T, Takahashi M, Shibata M, Tsuji G, Hatachi S . Presence of anti-nuclear antibodies is a risk factor for the appearance of anti-drug antibodies during infliximab or adalimumab therapy in patients with rheumatoid arthritis. PLoS One. 2020; 15(12):e0243729. PMC: 7735569. DOI: 10.1371/journal.pone.0243729. View

Sanchez Noriega J, Chartrand N, Valdoz J, Cribbs C, Jacobs D, Poulson D . Spatially and optically tailored 3D printing for highly miniaturized and integrated microfluidics. Nat Commun. 2021; 12(1):5509. PMC: 8448845. DOI: 10.1038/s41467-021-25788-w. View

Costa B, Coelho A, Beauchamp M, Nielsen J, Nordin G, Woolley A . 3D-printed microchip electrophoresis device containing spiral electrodes for integrated capacitively coupled contactless conductivity detection. Anal Bioanal Chem. 2021; 414(1):545-550. PMC: 8748415. DOI: 10.1007/s00216-021-03494-2. View

10.

Platchek M, Lu Q, Tran H, Xie W . Comparative Analysis of Multiple Immunoassays for Cytokine Profiling in Drug Discovery. SLAS Discov. 2020; 25(10):1197-1213. DOI: 10.1177/2472555220954389. View

11.

Nielsen A, Beauchamp M, Nordin G, Woolley A . 3D Printed Microfluidics. Annu Rev Anal Chem (Palo Alto Calif). 2019; 13(1):45-65. PMC: 7282950. DOI: 10.1146/annurev-anchem-091619-102649. View

12.

Yang W, Yu M, Sun X, Woolley A . Microdevices integrating affinity columns and capillary electrophoresis for multibiomarker analysis in human serum. Lab Chip. 2010; 10(19):2527-33. PMC: 2998056. DOI: 10.1039/c005288d. View

13.

Yang W, Sun X, Pan T, Woolley A . Affinity monolith preconcentrators for polymer microchip capillary electrophoresis. Electrophoresis. 2008; 29(16):3429-35. PMC: 2603467. DOI: 10.1002/elps.200700704. View

14.

Pandey C, Augustine S, Kumar S, Kumar S, Nara S, Srivastava S . Microfluidics Based Point-of-Care Diagnostics. Biotechnol J. 2017; 13(1). DOI: 10.1002/biot.201700047. View

15.

Svec F, Lv Y . Advances and recent trends in the field of monolithic columns for chromatography. Anal Chem. 2014; 87(1):250-73. DOI: 10.1021/ac504059c. View

16.

Andjelkovic U, Tufegdzic S, Popovic M . Use of monolithic supports for high-throughput protein and peptide separation in proteomics. Electrophoresis. 2017; 38(22-23):2851-2869. DOI: 10.1002/elps.201700260. View

17.

Estep P, Reid F, Nauman C, Liu Y, Sun T, Sun J . High throughput solution-based measurement of antibody-antigen affinity and epitope binning. MAbs. 2013; 5(2):270-8. PMC: 3893237. DOI: 10.4161/mabs.23049. View

18.

Furuya H, Tabula L, Lee R, Kralovec P, Ramsden M, Wong R . Analytical validation of ONCURIA™ a multiplex bead-based immunoassay for the non-invasive bladder cancer detection. Pract Lab Med. 2020; 22:e00189. PMC: 7691749. DOI: 10.1016/j.plabm.2020.e00189. View

19.

Abdiche Y, Malashock D, Pinkerton A, Pons J . Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet. Anal Biochem. 2008; 377(2):209-17. DOI: 10.1016/j.ab.2008.03.035. View

20.

Nielsen J, Nielsen A, Carson R, Lin H, Hanson R, Sonker M . Analysis of thrombin-antithrombin complex formation using microchip electrophoresis and mass spectrometry. Electrophoresis. 2019; 40(21):2853-2859. PMC: 6829041. DOI: 10.1002/elps.201900235. View