Altered Expression of MiR-29a-3p and MiR-34a-5p by Specific Inhibition of GSK3β in the MPP Treated SH-SY5Y Parkinson's Model

Overview

Journal Noncoding RNA Res

Date 2022 Jan 28

PMID 35087989

Authors

Morteza Ahmadzadeh-Darinsoo

Mojtaba Ahmadzadeh-Darinsoo

Shahsanam Abbasi

Ehsan Arefian

Claude Bernard

Azita Parvaneh Tafreshi

Affiliations

Soon will be listed here.

Abstract

In the current study, the effects of 7-BIO as a specific GSK3β inhibitor was examined on cell survival and expression of miR-29a-3p and miR-34a-5p in neurotoxin MPP treated SH-SY5Y cells. Our findings revealed that while co-treatment of the cells with 7-BIO and MPP did not alter the toxicity induced by MPP, pretreatment with 3.5 μM 7-BIO for 6 h increased the survival of the 2 mM MPP treated cells. Also, qRT-PCR analysis of gene expression showed that while miR-29a-3p was unchanged in cells treated with either 2 mM MPP or 3.5 μM 7-BIO alone, miR-34a-5p was increased by MPP but decreased by 7-BIO. Pretreatment with 3.5 μM 7-BIO prior to MPP however, increased miR-29a-3p but decreased miR-34a-5p induced by MPP. We therefore suggest that 7-BIO inhibition of GSK3β alleviates the MPP induced neurotoxicity by regulating miR-29a-3p and miR-34a-5p expressions in Parkinson's disease model SH-SY5Y cells.

Citing Articles

Circulating plasma miR-23b-3p as a biomarker target for idiopathic Parkinson's disease: comparison with small extracellular vesicle miRNA.

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PMID: 38033547 PMC: 10684698. DOI: 10.3389/fnins.2023.1174951.

References

Shanesazzade Z, Peymani M, Ghaedi K, Nasr Esfahani M . miR-34a/BCL-2 signaling axis contributes to apoptosis in MPP -induced SH-SY5Y cells. Mol Genet Genomic Med. 2018; 6(6):975-981. PMC: 6305653. DOI: 10.1002/mgg3.469. View

Chen L, Huang C, Shentu J, Wang M, Yan S, Zhou F . Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio) Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice. Front Mol Neurosci. 2017; 10:393. PMC: 5712304. DOI: 10.3389/fnmol.2017.00393. View

Margis R, Margis R, R M Rieder C . Identification of blood microRNAs associated to Parkinsonĭs disease. J Biotechnol. 2011; 152(3):96-101. DOI: 10.1016/j.jbiotec.2011.01.023. View

Mapa M, Le V, Wimalasena K . Characteristics of the mitochondrial and cellular uptake of MPP+, as probed by the fluorescent mimic, 4'I-MPP. PLoS One. 2018; 13(8):e0197946. PMC: 6107127. DOI: 10.1371/journal.pone.0197946. View

Javitch J, DAmato R, Strittmatter S, Snyder S . Parkinsonism-inducing neurotoxin, N-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine: uptake of the metabolite N-methyl-4-phenylpyridine by dopamine neurons explains selective toxicity. Proc Natl Acad Sci U S A. 1985; 82(7):2173-7. PMC: 397515. DOI: 10.1073/pnas.82.7.2173. View

Bove J, Perier C . Neurotoxin-based models of Parkinson's disease. Neuroscience. 2011; 211:51-76. DOI: 10.1016/j.neuroscience.2011.10.057. View

Schinelli S, Zuddas A, Kopin I, Barker J, di Porzio U . 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine metabolism and 1-methyl-4-phenylpyridinium uptake in dissociated cell cultures from the embryonic mesencephalon. J Neurochem. 1988; 50(6):1900-7. DOI: 10.1111/j.1471-4159.1988.tb02495.x. View

Dorsey E, Constantinescu R, Thompson J, Biglan K, Holloway R, Kieburtz K . Projected number of people with Parkinson disease in the most populous nations, 2005 through 2030. Neurology. 2006; 68(5):384-6. DOI: 10.1212/01.wnl.0000247740.47667.03. View

Arenas E, Salto C, Villaescusa C . WNT signaling in midbrain dopaminergic neuron development and cell replacement therapies for Parkinson's disease. Springerplus. 2016; 4(Suppl 1):L49. PMC: 4797852. DOI: 10.1186/2193-1801-4-S1-L49. View

10.

Meijer L, Skaltsounis A, Magiatis P, Polychronopoulos P, Knockaert M, Leost M . GSK-3-selective inhibitors derived from Tyrian purple indirubins. Chem Biol. 2004; 10(12):1255-66. DOI: 10.1016/j.chembiol.2003.11.010. View

11.

Fernandez-Santiago R, Iranzo A, Gaig C, Serradell M, Fernandez M, Tolosa E . MicroRNA association with synucleinopathy conversion in rapid eye movement behavior disorder. Ann Neurol. 2015; 77(5):895-901. DOI: 10.1002/ana.24384. View

12.

Akhlaghpour A, Parvaneh Tafreshi A, Roussa E, Bernard C, Zeynali B . TGFβ and Wnt Signaling Pathways Cooperatively Enhance Early Dopaminergic Differentiation of the Unrestricted Somatic Stem Cells. J Mol Neurosci. 2020; 70(5):769-777. DOI: 10.1007/s12031-020-01487-x. View

13.

Feng Z, Zhang C, Wu R, Hu W . Tumor suppressor p53 meets microRNAs. J Mol Cell Biol. 2011; 3(1):44-50. PMC: 3030969. DOI: 10.1093/jmcb/mjq040. View

14.

Langston J, Ballard P, Tetrud J, Irwin I . Chronic Parkinsonism in humans due to a product of meperidine-analog synthesis. Science. 1983; 219(4587):979-80. DOI: 10.1126/science.6823561. View

15.

Alural B, Ozerdem A, Allmer J, Genc K, Genc S . Lithium protects against paraquat neurotoxicity by NRF2 activation and miR-34a inhibition in SH-SY5Y cells. Front Cell Neurosci. 2015; 9:209. PMC: 4446540. DOI: 10.3389/fncel.2015.00209. View

16.

Nicklas W, Vyas I, Heikkila R . Inhibition of NADH-linked oxidation in brain mitochondria by 1-methyl-4-phenyl-pyridine, a metabolite of the neurotoxin, 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine. Life Sci. 1985; 36(26):2503-8. DOI: 10.1016/0024-3205(85)90146-8. View

17.

Kim T, Piao J, Koo S, Kriks S, Chung S, Betel D . Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use. Cell Stem Cell. 2021; 28(2):343-355.e5. PMC: 8006469. DOI: 10.1016/j.stem.2021.01.005. View

18.

Jain A, Jaiswal A . GSK-3beta acts upstream of Fyn kinase in regulation of nuclear export and degradation of NF-E2 related factor 2. J Biol Chem. 2007; 282(22):16502-10. DOI: 10.1074/jbc.M611336200. View

19.

Botta-Orfila T, Morato X, Compta Y, Lozano J, Falgas N, Valldeoriola F . Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson's disease. J Neurosci Res. 2014; 92(8):1071-7. DOI: 10.1002/jnr.23377. View

20.

Li J, Wang K, Chen X, Meng H, Song M, Wang Y . Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. BMC Mol Biol. 2012; 13:4. PMC: 3311059. DOI: 10.1186/1471-2199-13-4. View