Analogs of the DnaB Gene of Escherichia Coli K-12 Associated with Conjugative R Plasmids

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Jun 1

PMID 350846

Citations 8

Authors

P Y Wang

V N Iyer

Affiliations

Soon will be listed here.

Abstract

The dnaB266(Am) mutation in Escherichia coli K-12 is an amber mutation such that strains carrying this mutation are not viable in a sup+ strain. With five different R plasmids, it has been possible to construct viable R+ derivatives of this amber mutant and show that the plasmids themselves do not carry amber suppressors. This is interpreted as evidence for the presence of dnaB analog genes associated with these plasmids. Plasmid-positive strains carrying these genes often showed some degree of cryosensitivity of DNA synthesis and colony-forming ability. These observations indicate that the presence of dnaB analog genes in association with R plasmids must be relevant to the plasmid state or to some aspect of conjugative ability.

Citing Articles

Suppression of induction of SOS functions in an Escherichia coli tif-1 mutant by plasmid R100.1.

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dnaB analog function associated with plasmid R100drd-1.

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Processing of plasmid DNA during bacterial conjugation.

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Sequence of the dnaB gene of Salmonella typhimurium.

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DNA-protein interactions during replication of genetic elements of bacteria.

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References

Schuster H, Schlicht M, LANKA E, Mikolajczyk M, Edelbluth C . DNA synthesis in an Escherichia coli dna B dnaC mutant. Mol Gen Genet. 1977; 151(1):11-6. DOI: 10.1007/BF00446907. View

Wang P, Iyer V . Suppression and enhancement of temperature sensitivity of dnaB mutations of Escherichia coli K12 by conjugative plasmids. Plasmid. 1977; 1(1):19-33. DOI: 10.1016/0147-619x(77)90005-1. View

Novick R . Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969; 33(2):210-63. PMC: 378321. DOI: 10.1128/br.33.2.210-263.1969. View

Wickner S, Wright M, Hurwitz J . Association of DNA-dependent and -independent ribonucleoside triphosphatase activities with dnaB gene product of Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(3):783-7. PMC: 388098. DOI: 10.1073/pnas.71.3.783. View

Louarn J, Funderburgh M, Bird R . More precise mapping of the replication origin in Escherichia coli K-12. J Bacteriol. 1974; 120(1):1-5. PMC: 245722. DOI: 10.1128/jb.120.1.1-5.1974. View

Schuster H, Mikolajczyk M, Rohrschneider J, Geschke B . phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1975; 72(10):3907-11. PMC: 433105. DOI: 10.1073/pnas.72.10.3907. View

Gross J . DNA replication in bacteria. Curr Top Microbiol Immunol. 1972; 57:39-74. DOI: 10.1007/978-3-642-65297-4_2. View

Rosner J . Formation, induction, and curing of bacteriophage P1 lysogens. Virology. 1972; 48(3):679-89. DOI: 10.1016/0042-6822(72)90152-3. View

Kourilsky P, Leidner J, TREMBLAY G . DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea. Biochimie. 1971; 53(10):1111-4. DOI: 10.1016/s0300-9084(71)80201-8. View

10.

Ikeda H, Tomizawa J . Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968; 33:791-8. DOI: 10.1101/sqb.1968.033.01.091. View

11.

Brunk C, Leick V . Rapid equilibrium isopycnic CsC1 gradients. Biochim Biophys Acta. 1969; 179(1):136-44. DOI: 10.1016/0005-2787(69)90129-4. View

12.

Palchoudhury S, Iyer V . Compatibility between two F' factors in an Escherichia coli strain bearing a chromosomal mutation affecting DNA synthesis. J Mol Biol. 1971; 57(2):319-33. DOI: 10.1016/0022-2836(71)90349-4. View

13.

Kogoma T . Two types of temperature sensitivity in DNA replication of an Escherichia coli dnaB mutant. J Mol Biol. 1976; 103(1):191-7. DOI: 10.1016/0022-2836(76)90059-0. View

14.

Lark K, Wechsler J . DNA replication in dnaB mutants of Escherichia coli: gene product interaction and synthesis of 4 S pieces. J Mol Biol. 1975; 92(1):145-63. DOI: 10.1016/0022-2836(75)90095-9. View

15.

Bezanson G, Iyer V . dnaB gene of Escherichia coli K-12 affects superinfection inhibition between F' plasmids. J Bacteriol. 1975; 123(1):137-46. PMC: 235700. DOI: 10.1128/jb.123.1.137-146.1975. View

16.

Wickner S, Hurwitz J . Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro. Proc Natl Acad Sci U S A. 1975; 72(3):921-5. PMC: 432433. DOI: 10.1073/pnas.72.3.921. View

17.

Dari R, Yarmolinsky M . A dnaB analog specified by bacteriophage P1. J Mol Biol. 1975; 94(3):341-66. DOI: 10.1016/0022-2836(75)90207-7. View

18.

Ogawa T . Analysis of dnaB function of Escherichia coli K12 and the dnaB-like function of P1 prophage. J Mol Biol. 1975; 94(3):327-40. DOI: 10.1016/0022-2836(75)90206-5. View

19.

Hedges R, Jacob A, Barth P, Grinter N . Compatibility properties of P1 and PhiAMP prophages. Mol Gen Genet. 1975; 141(3):263-7. DOI: 10.1007/BF00341804. View

20.

Davis B, MINGIOLI E . Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950; 60(1):17-28. PMC: 385836. DOI: 10.1128/jb.60.1.17-28.1950. View