The Effect of Shear Stress on Cardiac Differentiation of Mesenchymal Stem Cells

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2022 Jan 25

PMID 35076851

Authors

Peyman Izadpanah

Ali Golchin

Tahereh Firuzyar

Masoud Najafi

Ali Jangjou

Sheida Hashemi

Affiliations

Soon will be listed here.

Abstract

Background: Stem cell therapy is developing as a valuable therapeutic trend for heart diseases. Most recent studies are aimed to find the most appropriate types of stem cells for the treatment of myocardial infarction (MI). The animal models have shown that bone marrow-derived mesenchymal stem cells (BMSCs) are a possible, safe, and efficient type of stem cell used in MI. The previous study demonstrated that 5-Azacytidine (5-Aza) could promote cardiac differentiation in stem cells.

Methods: This study used 5-Aza to induce cardiomyocyte differentiation in BMSCs both in static and microfluidic cell culture systems. For this purpose, we investigated the differentiation by using real-time PCR and Immunocytochemistry (ICC) Analysis.

Results: Our results showed that 5-Aza could cause to express cardiac markers in BMSCs as indicated by real-time PCR and immunocytochemistry (ICC). However, BMSCs are exposed to both 5-Aza and shear stress, and their synergistic effects could significantly induce cardiac gene expressions in BMSCs. This level of gene expression was observed neither in 5-Aza nor in shear stress groups only.

Conclusions: These results demonstrate that when BMSCs expose to 5-Aza as well as mechanical cues such as shear stress, the cardiac gene expression can be increased dramatically.

Citing Articles

Rebuilding the myocardial microenvironment to enhance mesenchymal stem cells-mediated regeneration in ischemic heart disease.

Chu Q, Jiang X, Xiao Y Front Bioeng Biotechnol. 2024; 12:1468833.

PMID: 39372432 PMC: 11452912. DOI: 10.3389/fbioe.2024.1468833.

Priming mesenchymal stem cells to develop "super stem cells".

Haider K World J Stem Cells. 2024; 16(6):623-640.

PMID: 38948094 PMC: 11212549. DOI: 10.4252/wjsc.v16.i6.623.

[Biological function of bladder smooth muscle cells regulated by multi-modal biomimetic stress].

Wei T, Chen L, Hu H, Yang J Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2024; 41(2):321-327.

PMID: 38686413 PMC: 11058499. DOI: 10.7507/1001-5515.202306036.

Exploring Electrospun Scaffold Innovations in Cardiovascular Therapy: A Review of Electrospinning in Cardiovascular Disease.

Broadwin M, Imarhia F, Oh A, Stone C, Sellke F, Bhowmick S Bioengineering (Basel). 2024; 11(3).

PMID: 38534492 PMC: 10968529. DOI: 10.3390/bioengineering11030218.

How the mechanical microenvironment of stem cell growth affects their differentiation: a review.

Zhang X, Zhang S, Wang T Stem Cell Res Ther. 2022; 13(1):415.

PMID: 35964140 PMC: 9375355. DOI: 10.1186/s13287-022-03070-0.

References

Xu Y, Guan J . Biomaterial property-controlled stem cell fates for cardiac regeneration. Bioact Mater. 2018; 1(1):18-28. PMC: 5883968. DOI: 10.1016/j.bioactmat.2016.03.002. View

Chen Y, Wang C, Huang Q, Wu D, Cao J, Xu X . Caveolin-1 Plays an Important Role in the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Cardiomyocytes. Cardiology. 2016; 136(1):40-48. DOI: 10.1159/000446869. View

Giacca M . Cardiac Regeneration After Myocardial Infarction: an Approachable Goal. Curr Cardiol Rep. 2020; 22(10):122. PMC: 7417409. DOI: 10.1007/s11886-020-01361-7. View

Furtado M, Nim H, Boyd S, Rosenthal N . View from the heart: cardiac fibroblasts in development, scarring and regeneration. Development. 2016; 143(3):387-97. DOI: 10.1242/dev.120576. View

Ardeshirylajimi A, Golchin A, Khojasteh A, Bandehpour M . Increased osteogenic differentiation potential of MSCs cultured on nanofibrous structure through activation of Wnt/β-catenin signalling by inorganic polyphosphate. Artif Cells Nanomed Biotechnol. 2018; 46(sup3):S943-S949. DOI: 10.1080/21691401.2018.1521816. View

Antonitsis P, Ioannidou-Papagiannaki E, Kaidoglou A, Papakonstantinou C . In vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. The role of 5-azacytidine. Interact Cardiovasc Thorac Surg. 2007; 6(5):593-7. DOI: 10.1510/icvts.2007.157875. View

Rose R, Jiang H, Wang X, Helke S, Tsoporis J, Gong N . Bone marrow-derived mesenchymal stromal cells express cardiac-specific markers, retain the stromal phenotype, and do not become functional cardiomyocytes in vitro. Stem Cells. 2008; 26(11):2884-92. DOI: 10.1634/stemcells.2008-0329. View

Yu Y, Gao Q, Zhao H, Li R, Gao J, Ding T . Erratum to: Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region. Stem Cell Res Ther. 2015; 6:180. PMC: 4574565. DOI: 10.1186/s13287-015-0161-7. View

Kim M, Kino-Oka M, Maruyama N, Saito A, Sawa Y, Taya M . Cardiomyogenic induction of human mesenchymal stem cells by altered Rho family GTPase expression on dendrimer-immobilized surface with D-glucose display. Biomaterials. 2010; 31(30):7666-77. DOI: 10.1016/j.biomaterials.2010.06.034. View

10.

Zhang B, Shen L, Shi H, Pan Z, Wu L, Yan Y . Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics. Stem Cells Int. 2017; 2016:1929536. PMC: 5220513. DOI: 10.1155/2016/1929536. View

11.

Gheisari F, Shafiee M, Abbasi M, Jangjou A, Izadpanah P, Vaez A . Janus nanoparticles: an efficient intelligent modern nanostructure for eradicating cancer. Drug Metab Rev. 2021; 53(4):592-603. DOI: 10.1080/03602532.2021.1878530. View

12.

Zhang Y, Aleman J, Arneri A, Bersini S, Piraino F, Shin S . From cardiac tissue engineering to heart-on-a-chip: beating challenges. Biomed Mater. 2015; 10(3):034006. PMC: 4489846. DOI: 10.1088/1748-6041/10/3/034006. View

13.

Verhulsel M, Vignes M, Descroix S, Malaquin L, Vignjevic D, Viovy J . A review of microfabrication and hydrogel engineering for micro-organs on chips. Biomaterials. 2013; 35(6):1816-32. DOI: 10.1016/j.biomaterials.2013.11.021. View

14.

Vaez S, Ebrahimi-Barough S, Soleimani M, Kolivand S, Farzamfar S, Ahmadi Tafti S . The cardiac niche role in cardiomyocyte differentiation of rat bone marrow-derived stromal cells: comparison between static and microfluidic cell culture methods. EXCLI J. 2018; 17:762-774. PMC: 6123612. DOI: 10.17179/excli2018-1539. View

15.

Huang Y, Jia X, Bai K, Gong X, Fan Y . Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells. Arch Med Res. 2010; 41(7):497-505. DOI: 10.1016/j.arcmed.2010.10.002. View

16.

Khajeniazi S, Solati M, Yazdani Y, Soleimani M, Kianmehr A . Synergistic induction of cardiomyocyte differentiation from human bone marrow mesenchymal stem cells by interleukin 1β and 5-azacytidine. Biol Chem. 2016; 397(12):1355-1364. DOI: 10.1515/hsz-2016-0151. View

17.

Yang M, Wang S, Chou N, Chi N, Huang Y, Chang Y . The cardiomyogenic differentiation of rat mesenchymal stem cells on silk fibroin-polysaccharide cardiac patches in vitro. Biomaterials. 2009; 30(22):3757-65. DOI: 10.1016/j.biomaterials.2009.03.057. View

18.

Petropoulou E, Soltani M, Dehghani Firoozabadi A, Namayandeh S, Crockford J, Maroofian R . Digenic inheritance of mutations in the cardiac troponin (TNNT2) and cardiac beta myosin heavy chain (MYH7) as the cause of severe dilated cardiomyopathy. Eur J Med Genet. 2017; 60(9):485-488. DOI: 10.1016/j.ejmg.2017.06.008. View

19.

Yang G, Song M, Hoang D, Tran Q, Choe W, Kang I . Melatonin prevents doxorubicin-induced cardiotoxicity through suppression of AMPKα2-dependent mitochondrial damage. Exp Mol Med. 2020; 52(12):2055-2068. PMC: 8080573. DOI: 10.1038/s12276-020-00541-3. View

20.

Kong C, Bursac N, Tung L . Mechanoelectrical excitation by fluid jets in monolayers of cultured cardiac myocytes. J Appl Physiol (1985). 2005; 98(6):2328-36. DOI: 10.1152/japplphysiol.01084.2004. View