Dandelion (Taraxacum Officinale) Improves the Therapeutic Efficiency of Praziquantel in Experimental Schistosomiasis

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2022 Jan 24

PMID 35067863

Authors

Marwa Th M Atwa

Areeg M Abd-Elrazek

Noha I S Salem

Affiliations

Soon will be listed here.

Abstract

Purpose: Although praziquantel (PZQ) has a wide use as an anti-schistosome agent, many of its imperfections and side effects have been reported in many studies. The current study aims to evaluate the curative effect of a natural dandelion extract (Taraxacum officinale) on schistosomiasis either alone or in combination with PZQ based on parasitological, immunological, histopathological and molecular investigations.

Methods: Mice were experimentally infected with Schistosoma mansoni cercariae and then divided into four groups, Schistosoma spp.-infected untreated group (IC group), Schistosoma spp.-infected group of mice treated with dandelion (I-Dn group), Schistosoma spp.-infected group of mice treated with PZQ (I-PZQ group), and Schistosoma spp.-infected group of mice treated with both PZQ and dandelion (I-PZQ + Dn group). Treatment started 45 days' post-infection. Besides, non-infected, non-treated mice served as the negative healthy control group (HC group).

Results: The present results indicated that dandelion administration significantly reduced the worm burden, ova number, and the number and diameter of hepatic granulomas as compared to the untreated infected group. The results also showed that the levels of IL-6 and TNF-α were significantly decreased in the combined treatment group (I-PZQ + Dn) as compared to the I-PZQ group. Administration of dandelion-only remarkably reduced AST and ALT activities associated with schistosomiasis. Moreover, hepatic DNA damage assessed by comet assay was significantly inhibited in the combined treated group compared to the infected untreated and PZQ treated groups.

Conclusion: The results concluded that combined treatment of PZQ and dandelion extract improved immune response, decreased the number and diameter of granulomas, and inhibited DNA damage, indicating a reduction in liver fibrosis associated with schistosomiasis. The present study focused on the potential effect of dandelion as an adjunct medication for therapeutic properties of PZQ.

Citing Articles

Mechanistic Role of Methanolic Extract of Taraxacum officinale Roots as Cardioprotective Against Ischemia-Reperfusion Injury-Induced Myocardial Infarction in Rats.

Sharma M, Pal P, Pottoo F, Kumar S Appl Biochem Biotechnol. 2023; 195(5):3384-3405.

PMID: 36595191 DOI: 10.1007/s12010-022-04282-z.

References

Warren K, Mahmoud A, Cummings P, Murphy D, HOUSER H . Schistosomiasis mansoni in Yemeni in California: duration of infection, presence of disease, therapeutic management. Am J Trop Med Hyg. 1974; 23(5):902-9. DOI: 10.4269/ajtmh.1974.23.902. View

McManus D, Dunne D, Sacko M, Utzinger J, Vennervald B, Zhou X . Schistosomiasis. Nat Rev Dis Primers. 2018; 4(1):13. DOI: 10.1038/s41572-018-0013-8. View

Fallon P, Tao L, Ismail M, Bennett J . Schistosome resistance to praziquantel: Fact or artifact?. Parasitol Today. 1996; 12(8):316-20. DOI: 10.1016/0169-4758(96)10029-6. View

De Oliveira R, Rehder V, Santos Oliveira A, Lourdes Sierpe Jeraldo V, Linhares A, Allegretti S . Anthelmintic activity in vitro and in vivo of Baccharis trimera (Less) DC against immature and adult worms of Schistosoma mansoni. Exp Parasitol. 2014; 139:63-72. DOI: 10.1016/j.exppara.2014.02.010. View

Mahmoud T, Rizk S, Maghraby A, Shaheen A . Propolis enhances the effectiveness of praziquantel in experimental schistosomiasis: biochemical and histopathological study. Parasitol Res. 2014; 113(12):4513-23. DOI: 10.1007/s00436-014-4141-0. View

Bae M, Park Y, Lee J . Food components with antifibrotic activity and implications in prevention of liver disease. J Nutr Biochem. 2017; 55:1-11. PMC: 5936673. DOI: 10.1016/j.jnutbio.2017.11.003. View

Kalny P, Fijalek Z, Daszczuk A, Ostapczuk P . Determination of selected microelements in polish herbs and their infusions. Sci Total Environ. 2007; 381(1-3):99-104. DOI: 10.1016/j.scitotenv.2007.03.026. View

Wink M . Medicinal plants: a source of anti-parasitic secondary metabolites. Molecules. 2012; 17(11):12771-91. PMC: 6268567. DOI: 10.3390/molecules171112771. View

Schutz K, Carle R, Schieber A . Taraxacum--a review on its phytochemical and pharmacological profile. J Ethnopharmacol. 2006; 107(3):313-23. DOI: 10.1016/j.jep.2006.07.021. View

10.

Wirngo F, Lambert M, Jeppesen P . The Physiological Effects of Dandelion (Taraxacum Officinale) in Type 2 Diabetes. Rev Diabet Stud. 2016; 13(2-3):113-131. PMC: 5553762. DOI: 10.1900/RDS.2016.13.113. View

11.

Rehman G, Hamayun M, Iqbal A, Khan S, Khan H, Shehzad A . Effect of Methanolic Extract of Dandelion Roots on Cancer Cell Lines and AMP-Activated Protein Kinase Pathway. Front Pharmacol. 2017; 8:875. PMC: 5712354. DOI: 10.3389/fphar.2017.00875. View

12.

Taper H, Roberfroid M . Influence of inulin and oligofructose on breast cancer and tumor growth. J Nutr. 1999; 129(7 Suppl):1488S-91S. DOI: 10.1093/jn/129.7.1488S. View

13.

Arpadjan S, Celik G, Taskesen S, Gucer S . Arsenic, cadmium and lead in medicinal herbs and their fractionation. Food Chem Toxicol. 2008; 46(8):2871-5. DOI: 10.1016/j.fct.2008.05.027. View

14.

Piper K, Mott R, McLaren D . Schistosoma mansoni: histological analysis of the synergistic interaction between vaccine immunity and praziquantel therapy in the lungs of mice. Parasite Immunol. 1990; 12(4):367-87. DOI: 10.1111/j.1365-3024.1990.tb00975.x. View

15.

Smithers S, Terry R . The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965; 55(4):695-700. DOI: 10.1017/s0031182000086248. View

16.

CHEEVER A . Conditions affecting the accuracy of potassium hydroxide digestion techniques for counting Schistosoma mansoni eggs in tissues. Bull World Health Organ. 1968; 39(2):328-31. PMC: 2554554. View

17.

Pellegrino J, Faria J . THE OOGRAM METHOD FOR THE SCREENING OF DRUGS IN SCHISTOSOMIASIS MANSONI. Am J Trop Med Hyg. 1965; 14:363-9. DOI: 10.4269/ajtmh.1965.14.363. View

18.

Jacobs W, Bogers J, Deelder A, Wery M, Marck E . Adult Schistosoma mansoni worms positively modulate soluble egg antigen-induced inflammatory hepatic granuloma formation in vivo. Stereological analysis and immunophenotyping of extracellular matrix proteins, adhesion molecules, and chemokines. Am J Pathol. 1997; 150(6):2033-45. PMC: 1858301. View

19.

Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3):206-21. DOI: 10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j. View

20.

Dongoran R, Lin T, Byekyet A, Tang S, Yang J, Liu C . Determination of Major Endogenous FAHFAs in Healthy Human Circulation: The Correlations with Several Circulating Cardiovascular-Related Biomarkers and Anti-Inflammatory Effects on RAW 264.7 Cells. Biomolecules. 2020; 10(12). PMC: 7766943. DOI: 10.3390/biom10121689. View