Long Non-coding RNA Terminal Differentiation-induced Non-coding RNA Regulates Cisplatin Resistance of Choroidal Melanoma by Positively Modulating Extracellular Signal-regulated Kinase 2 Via Sponging MicroRNA-19b-3p

Overview

Journal Bioengineered

Date 2022 Jan 24

PMID 35067169

Authors

Wei Li

Aiqin Nie

Longyu Jin

Yubo Cui

Ning Xie

Gaohua Liang

Affiliations

Soon will be listed here.

Abstract

In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide (CCK-8) assay was utilized to detect chemosensitivity and cell viability. Flow cytometry analysis was performed to detect apoptotic cells. The protein levels of Bax, Bcl-2, cleaved-caspase-3, ERK-2, and nuclear factor-kappa B p65 were measured by Western blot. RNA immunoprecipitation (RIP) and dual-luciferase reporter assays were performed to determine the relationship among TINCR, miR-19b-3p, and ERK-2. The results showed that the levels of TINCR and ERK-2 were markedly increased in DDP-resistant CM tissues and cells, while miR-19b-3p level was significantly reduced. TINCR knockdown reduced DDP resistance and cell viability and promoted cell apoptosis, while TINCR overexpression exhibited opposite effects. TINCR and ERK-2 were direct targets of miR-19b-3p. Further experiments revealed that TINCR enhanced DDP resistance in CM cells by regulating the miR-19b-3p/ERK-2/NF-kb axis. Taken together, our study revealed a critical role of TINCR in regulating DDP resistance in CM and suggested that TINCR is a potential cisplatin-resistant CM therapeutic target.

Citing Articles

Non-coding RNA in cancer drug resistance: Underlying mechanisms and clinical applications.

Zhou X, Ao X, Jia Z, Li Y, Kuang S, Du C Front Oncol. 2022; 12:951864.

PMID: 36059609 PMC: 9428469. DOI: 10.3389/fonc.2022.951864.

References

Gilbert C, Svejstrup J . RNA immunoprecipitation for determining RNA-protein associations in vivo. Curr Protoc Mol Biol. 2008; Chapter 27:Unit 27.4. DOI: 10.1002/0471142727.mb2704s75. View

Jiang Q, Fu Q, Chang Y, Liu Z, Zhang J, Xu L . CD19 tumor-infiltrating B-cells prime CD4 T-cell immunity and predict platinum-based chemotherapy efficacy in muscle-invasive bladder cancer. Cancer Immunol Immunother. 2018; 68(1):45-56. PMC: 11028136. DOI: 10.1007/s00262-018-2250-9. View

Gamazon E, Trendowski M, Wen Y, Wing C, Delaney S, Huh W . Gene and MicroRNA Perturbations of Cellular Response to Pemetrexed Implicate Biological Networks and Enable Imputation of Response in Lung Adenocarcinoma. Sci Rep. 2018; 8(1):733. PMC: 5768793. DOI: 10.1038/s41598-017-19004-3. View

Li F, Yoshizawa J, Kim K, Kanjanapangka J, Grogan T, Wang X . Discovery and Validation of Salivary Extracellular RNA Biomarkers for Noninvasive Detection of Gastric Cancer. Clin Chem. 2018; 64(10):1513-1521. PMC: 7720197. DOI: 10.1373/clinchem.2018.290569. View

Zhang X, Zhang W, Jiang Y, Liu K, Ran L, Song F . Identification of functional lncRNAs in gastric cancer by integrative analysis of GEO and TCGA data. J Cell Biochem. 2019; 120(10):17898-17911. DOI: 10.1002/jcb.29058. View

Wan Y, Yang Z . LncRNA NEAT1 affects inflammatory response by targeting miR-129-5p and regulating Notch signaling pathway in epilepsy. Cell Cycle. 2020; 19(4):419-431. PMC: 7100884. DOI: 10.1080/15384101.2020.1711578. View

Eissa S, Seada L . Quantitation of bcl-2 protein in bladder cancer tissue by enzyme immunoassay: comparison with Western blot and immunohistochemistry. Clin Chem. 1998; 44(7):1423-9. View

Maeda T, Yoshino K, Nagai K, Oaku S, Kato M, Hiura A . The efficacy of platinum-based chemotherapy for immune checkpoint inhibitor-resistant advanced melanoma. Acta Oncol. 2019; 58(3):379-381. DOI: 10.1080/0284186X.2018.1541252. View

Sarfi M, Abbastabar M, Khalili E . Long noncoding RNAs biomarker-based cancer assessment. J Cell Physiol. 2019; 234(10):16971-16986. DOI: 10.1002/jcp.28417. View

10.

Huang C, Chen Y, Wu-Hsieh B, Fang J, Chang Z . The Ca-loop in thymidylate kinase is critical for growth and contributes to pyrimidine drug sensitivity of . J Biol Chem. 2019; 294(27):10686-10697. PMC: 6615673. DOI: 10.1074/jbc.RA118.006798. View

11.

Li N, Meng D, Gao L, Xu Y, Liu P, Tian Y . Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression. Radiat Oncol. 2018; 13(1):210. PMC: 6201557. DOI: 10.1186/s13014-018-1153-4. View

12.

Zhu Y, Dong S, Zhu Y, Zhao Y, Xu Y . Identification of cancer prognosis-associated lncRNAs based on the miRNA-TF co-regulatory motifs and dosage sensitivity. Mol Omics. 2019; 15(5):361-373. DOI: 10.1039/c9mo00089e. View

13.

Jiang T, Ye L, Han Z, Liu Y, Yang Y, Peng Z . miR-19b-3p promotes colon cancer proliferation and oxaliplatin-based chemoresistance by targeting SMAD4: validation by bioinformatics and experimental analyses. J Exp Clin Cancer Res. 2017; 36(1):131. PMC: 5610468. DOI: 10.1186/s13046-017-0602-5. View

14.

Duan L, Duan D, Wei W, Sun Z, Xu H, Guo L . MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6. Mol Cell Biochem. 2019; 459(1-2):205-214. DOI: 10.1007/s11010-019-03563-2. View

15.

Zhao L, Li R, Han D, Zhang X, Nian G, Wu M . Independent prognostic Factor of low-expressed LncRNA ZNF667-AS1 for cervical cancer and inhibitory function on the proliferation of cervical cancer. Eur Rev Med Pharmacol Sci. 2017; 21(23):5353-5360. DOI: 10.26355/eurrev_201712_13920. View

16.

Shields C, Dalvin L, Yu M, Ancona-Lezama D, Di Nicola M, Williams B . CHOROIDAL NEVUS TRANSFORMATION INTO MELANOMA PER MILLIMETER INCREMENT IN THICKNESS USING MULTIMODAL IMAGING IN 2355 CASES: The 2019 Wendell L. Hughes Lecture. Retina. 2019; 39(10):1852-1860. DOI: 10.1097/IAE.0000000000002508. View

17.

Hamza H, Elhusseiny A . Choroidal Melanoma Resection. Middle East Afr J Ophthalmol. 2018; 25(2):65-70. PMC: 6071344. DOI: 10.4103/meajo.MEAJO_73_18. View

18.

Wang P, Chen D, Ma H, Li Y . LncRNA MEG3 enhances cisplatin sensitivity in non-small cell lung cancer by regulating miR-21-5p/SOX7 axis. Onco Targets Ther. 2017; 10:5137-5149. PMC: 5661845. DOI: 10.2147/OTT.S146423. View

19.

Li Y, Zhang J, Liu Y, Zhang B, Zhong F, Wang S . MiR-30a-5p confers cisplatin resistance by regulating IGF1R expression in melanoma cells. BMC Cancer. 2018; 18(1):404. PMC: 5896053. DOI: 10.1186/s12885-018-4233-9. View

20.

Schmittgen T, Livak K . Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3(6):1101-8. DOI: 10.1038/nprot.2008.73. View