Antioxidant and Lipid-reducing Effects of Root Extract in 3T3-L1 Cell

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2022 Jan 21

PMID 35059236

Authors

Da-Hye Choi

Joon-Hee Han

Min Hong

Sun-Yeop Lee

Soo-Ung Lee

Tae-Hyung Kwon

Affiliations

Soon will be listed here.

Abstract

root is traditionally known to be effective in the treatment of diabetes in Korea. root-specific compounds also show antioxidant effects, and could reduce lipid and fat accumulation, however, the underlying mechanism has not been clarified. In present study, the antioxidant and lipid-reducing effects of a 50% ethanol extract of (REE) were investigated differentiated mouse preadipocytes (3T3-L1 cells). REE showed strong radical scavenging activities and inhibitory effect of total lipid accumulation and triglyceride levels in differentiated 3T3-L1 cells. In addition, REE treatment reduced the mRNA and protein levels of adipogenesis and lipogenesis markers. This REE-promoted lipid reduction was caused by downregulation of peroxisome proliferator activated receptor gamma (), CCAAT/enhancer binding protein alpha (), and sterol regulatory element binding protein1 () and down regulation of ERK expression. Overall, these results demonstrate the potential of REE for development of a drug in the medical treatment of lipid-associated disorders.

Citing Articles

Inhibition of Differentiation of 3T3-L1 Cells by Increasing Glioma-Associated Oncogene Expression in L. Using KCTC 3115.

Cho Y, Lee J, Lee Y, Lee M, Choi J Prev Nutr Food Sci. 2025; 29(4):533-545.

PMID: 39759806 PMC: 11699586. DOI: 10.3746/pnf.2024.29.4.533.

The Root Extract of Ameliorates Nonalcoholic Steatohepatitis Development via Blockade of De Novo Lipogenesis and Inflammation.

Kim N, Lee S, Lee K, Song A, Park H, Kang J Curr Issues Mol Biol. 2024; 46(6):5881-5893.

PMID: 38921022 PMC: 11202599. DOI: 10.3390/cimb46060351.

Performance and mechanism of co-culture of Monascus purpureus, Lacticaseibacillus casei, and Saccharomyces cerevisiae to enhance lovastatin production and lipid-lowering effects.

Wu M, Wang Q, Zhang H, Pan Z, Zeng Q, Fang W Bioprocess Biosyst Eng. 2023; 46(10):1411-1426.

PMID: 37688635 DOI: 10.1007/s00449-023-02903-3.

Translational Research on Bee Pollen as a Source of Nutrients: A Scoping Review from Bench to Real World.

Kacemi R, Campos M Nutrients. 2023; 15(10).

PMID: 37242296 PMC: 10221365. DOI: 10.3390/nu15102413.

Nutraceuticals and the Network of Obesity Modulators.

Ammendola S, Scotto dAbusco A Nutrients. 2022; 14(23).

PMID: 36501129 PMC: 9739360. DOI: 10.3390/nu14235099.

References

Rodgers R, Tschop M, Wilding J . Anti-obesity drugs: past, present and future. Dis Model Mech. 2012; 5(5):621-6. PMC: 3424459. DOI: 10.1242/dmm.009621. View

Yun J . Possible anti-obesity therapeutics from nature--a review. Phytochemistry. 2010; 71(14-15):1625-41. DOI: 10.1016/j.phytochem.2010.07.011. View

Nakamura K, Miyoshi T, Yunoki K, Ito H . Postprandial hyperlipidemia as a potential residual risk factor. J Cardiol. 2016; 67(4):335-9. DOI: 10.1016/j.jjcc.2015.12.001. View

Besagil P, Calapkorur S, Sahin H . Determination of the relationship between total antioxidant capacity and dietary antioxidant intake in obese patients. Niger J Clin Pract. 2020; 23(4):481-488. DOI: 10.4103/njcp.njcp_212_19. View

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M . Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. DOI: 10.1016/s0891-5849(98)00315-3. View

Esposito K, Ciotola M, Schisano B, Misso L, Giannetti G, Ceriello A . Oxidative stress in the metabolic syndrome. J Endocrinol Invest. 2006; 29(9):791-5. DOI: 10.1007/BF03347372. View

Jose C, Melser S, Benard G, Rossignol R . Mitoplasticity: adaptation biology of the mitochondrion to the cellular redox state in physiology and carcinogenesis. Antioxid Redox Signal. 2012; 18(7):808-49. DOI: 10.1089/ars.2011.4357. View

Wu Z, Rosen E, Brun R, Hauser S, Adelmant G, Troy A . Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell. 1999; 3(2):151-8. DOI: 10.1016/s1097-2765(00)80306-8. View

Goran M, Ball G, Cruz M . Obesity and risk of type 2 diabetes and cardiovascular disease in children and adolescents. J Clin Endocrinol Metab. 2003; 88(4):1417-27. DOI: 10.1210/jc.2002-021442. View

10.

Choi D, Han J, Yu K, Hong M, Lee S, Park K . Antioxidant and Anti-Obesity Activities of Extract through Alleviation of Lipid Accumulation on 3T3-L1 Adipocytes. J Microbiol Biotechnol. 2019; 30(1):21-30. PMC: 9728287. DOI: 10.4014/jmb.1910.10040. View

11.

Aouadi M, Laurent K, Prot M, Le Marchand-Brustel Y, Binetruy B, Bost F . Inhibition of p38MAPK increases adipogenesis from embryonic to adult stages. Diabetes. 2006; 55(2):281-9. DOI: 10.2337/diabetes.55.02.06.db05-0963. View

12.

White U, Stephens J . Transcriptional factors that promote formation of white adipose tissue. Mol Cell Endocrinol. 2009; 318(1-2):10-4. PMC: 3079373. DOI: 10.1016/j.mce.2009.08.023. View

13.

Hamilton M, Hamilton D, Zderic T . Role of low energy expenditure and sitting in obesity, metabolic syndrome, type 2 diabetes, and cardiovascular disease. Diabetes. 2007; 56(11):2655-67. DOI: 10.2337/db07-0882. View

14.

Saklayen M . The Global Epidemic of the Metabolic Syndrome. Curr Hypertens Rep. 2018; 20(2):12. PMC: 5866840. DOI: 10.1007/s11906-018-0812-z. View

15.

Ahmad B, Serpell C, Fong I, Wong E . Molecular Mechanisms of Adipogenesis: The Anti-adipogenic Role of AMP-Activated Protein Kinase. Front Mol Biosci. 2020; 7:76. PMC: 7226927. DOI: 10.3389/fmolb.2020.00076. View

16.

Thao N, Luyen B, Jo S, Manh Hung T, Cuong N, Hoai Nam N . Triterpenoid saponins from the roots of Rosa rugosa Thunb. as rat intestinal sucrase inhibitors. Arch Pharm Res. 2014; 37(10):1280-5. DOI: 10.1007/s12272-014-0384-7. View

17.

Farmer S . Transcriptional control of adipocyte formation. Cell Metab. 2006; 4(4):263-73. PMC: 1958996. DOI: 10.1016/j.cmet.2006.07.001. View

18.

Huang C, McAllister M, Slusher A, Webb H, Mock J, Acevedo E . Obesity-Related Oxidative Stress: the Impact of Physical Activity and Diet Manipulation. Sports Med Open. 2015; 1(1):32. PMC: 4580715. DOI: 10.1186/s40798-015-0031-y. View

19.

Prusty D, Park B, Davis K, Farmer S . Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes. J Biol Chem. 2002; 277(48):46226-32. DOI: 10.1074/jbc.M207776200. View

20.

Choi K, Ghaddar B, Moya C, Shi H, Sridharan G, Lee K . Analysis of transcription factor network underlying 3T3-L1 adipocyte differentiation. PLoS One. 2014; 9(7):e100177. PMC: 4116336. DOI: 10.1371/journal.pone.0100177. View