Evaluation of the Complete Nuclear RDNA Unit Sequence of the Jellyfish Kishinouye (Scyphozoa: Semaeostomeae) for Molecular Discrimination

Overview

Journal Anim Cells Syst (Seoul)

Specialty Biology

Date 2022 Jan 21

PMID 35059142

Authors

Buhari Lawan Muhammad

Yoseph Seo

Jang-Seu Ki

Affiliations

Soon will be listed here.

Abstract

The harmful jellyfish Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean and characterized the molecular features of the rDNA. The complete rDNA contained 8,003 bp (48.4% GC) with the same gene arrangement (18S, ITS1, 5.8S, ITS2, 28S, and IGS) to the typical eukaryotes. Dot plot analysis showed that the coding regions (18S, 5.8S, and 28S) were highly conserved, while the non-coding regions (ITS1, ITS2, and IGS) were more variable and parsimony-informative. The IGS contained a putative transcription termination signal (poly(T) tract) and four repeats of block minisatellites. Phylogenetic analyses using 18S and 28S rDNA revealed well-resolved relationships of within the order Semaeostomeae, separating it from other species. The complete rDNA sequence provides various options for the selection of jellyfish taxonomic markers and may be useful for discriminating between species of and phylogeny reconstruction with close relatives.

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