A Compensatory Link Between Cleavage/polyadenylation and MRNA Turnover Regulates Steady-state MRNA Levels in Yeast

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2022 Jan 21

PMID 35058367

Authors

Zarmik Moqtaderi

Joseph V Geisberg

Kevin Struhl

Affiliations

Soon will be listed here.

Abstract

Cells have compensatory mechanisms to coordinate the rates of major biological processes, thereby permitting growth in a wide variety of conditions. Here, we uncover a compensatory link between cleavage/polyadenylation in the nucleus and messenger RNA (mRNA) turnover in the cytoplasm. On a global basis, same-gene 3' mRNA isoforms with twofold or greater differences in half-lives have steady-state mRNA levels that differ by significantly less than a factor of 2. In addition, increased efficiency of cleavage/polyadenylation at a specific site is associated with reduced stability of the corresponding 3' mRNA isoform. This inverse relationship between cleavage/polyadenylation and mRNA isoform half-life reduces the variability in the steady-state levels of mRNA isoforms, and it occurs in all four growth conditions tested. These observations suggest that during cleavage/polyadenylation in the nucleus, mRNA isoforms are marked in a manner that persists upon translocation to the cytoplasm and affects the activity of mRNA degradation machinery, thus influencing mRNA stability.

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