Melatonin Attenuates RANKL-Induced Osteoclastogenesis Via Inhibition of Atp6v0d2 and DC-STAMP Through MAPK and NFATc1 Signaling Pathways

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Jan 21

PMID 35056817

Authors

Seong-Sik Kim

Soon-Pill Jeong

Bong-Soo Park

In-Ryoung Kim

Affiliations

Soon will be listed here.

Abstract

Methods: the cytotoxic effect of melatonin was investigated by MTT assay. Osteoclast differentiation and gene expression of osteoclast-related factors were confirmed via TRAP staining, pit formation assay, immunofluorescence imaging, western blot, and real-time PCR.

Results: melatonin was found to inactivate the p38 and JNK of MAP kinase in RAW264.7 cells treated with RANKL and treated with a combination RANKL and melatonin for 1, 3, and 5 days. The melatonin treatment group showed a reduction in osteoclastogenesis transcription factors and ATP6v0d2 gene expression.

Conclusions: melatonin inhibits osteoclast differentiation and cell fusion by inhibiting the expression of Atp6v0d2 through the inactivation of MAPK and NFATc1 signaling in RANKL-stimulated RAW264.7 macrophages. The findings of the present study suggest that melatonin could be a suitable therapy for bone loss and imply a potential role of melatonin in bone health.

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