Whole-Genome Sequencing Characterization of Virulence Profiles of Food and Human Isolates and In Vitro Adhesion/Invasion Assessment

Overview

Journal Microorganisms

Specialty Microbiology

Date 2022 Jan 21

PMID 35056510

Authors

Giuditta Fiorella Schiavano

Collins Njie Ateba

Annalisa Petruzzelli

Veronica Mele

Giulia Amagliani

Fabrizia Guidi

Mauro De Santi

Francesco Pomilio

Giuliana Blasi

Antonietta Gattuso

Stefania Di Lullo

Elena Rocchegiani

Giorgio Brandi

Affiliations

Soon will be listed here.

Abstract

() is the causative agent of human listeriosis. strains have different virulence potential. For this reason, we preliminarily characterised via Whole-Genome Sequencing (WGS) some strains for their key genomic features and virulence-associated determinants, assigning the clonal complex (CC). Moreover, the ability of the same strains to adhere to and invade human colon carcinoma cell line Caco-2, evaluating the possible correspondence with their genetic virulence profile, was also assessed. The clinical strains typed belonged to clonal complex (CC)1, CC31, and CC101 and showed a very low invasiveness. The strains isolated from food were assigned to CC1, CC7, CC9, and CC121. All CC1 carried the hypervirulence pathogenicity island LIPI-3 in addition to LIPI-1. Premature stop codons in the gene were found only in of food origin belonging to CC9 and CC121. The presence of was observed in all the CCs except CC1. The CC7 strain, belonging to an epidemic cluster, also carried the internalin genes and and showed the highest level of invasion. In contrast, the human CC31 strain lacked the and genes and presented the lowest level of invasiveness. In , the genetic determinants of hypo- or hypervirulence are not necessarily predictive of a cell adhesion and/or invasion ability in vitro. Moreover, since listeriosis results from the interplay between host and virulence features of the pathogen, even hypovirulent clones are able to cause infection in immunocompromised people.

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