Epimerization of Deoxynivalenol by the Strain A6-243 Assisted by Pyrroloquinoline Quinone

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2022 Jan 20

PMID 35050993

Authors

Hui Gao

Jiafeng Niu

Hua Yang

Zhaoxin Lu

Libang Zhou

Fanqiang Meng

Fengxia Lu

Meirong Chen

Affiliations

Soon will be listed here.

Abstract

Deoxynivalenol (DON) is a secondary metabolite produced by several species that is hazardous to humans and animals after entering food chains. In this study, by adding cofactors, the strain A6-243 is identified as the DON-transforming bacteria from a bacterial consortium with the ability to biotransform DON of sp. B6-24 and strain A6-243, and its effect on the biotransformation process of DON is studied. The strain A6-243 completely biotransformed 100 μg/mL of DON with the assistance of the exogenous addition of PQQ (pyrroloquinoline quinone) within 48 h and produced non-toxic 3-epi-DON (3-epi-deoxynivalenol), while sp. B6-24 was not able to biotransform DON, but it had the ability to generate PQQ. Moreover, the strain A6-243 not only degraded DON, but also exhibited the ability to degrade 3-keto-DON (3-keto-deoxynivalenol) with the same product 3-epi-DON, indicating that DON epimerization by the strain A6-243 is a two-step enzymatic reaction. The most suitable conditions for the biodegradation process of the strain A6-243 were a temperature of 16-37 °C and pH 7.0-10, with 15-30 μM PQQ. In addition, the strain A6-243 was found to completely remove DON (6.7 μg/g) from DON-contaminated wheat. The results presented a reference for screening microorganisms with the ability of biotransform DON and laid a foundation for the development of enzymes for the detoxification of mycotoxins in grain and its products.

Citing Articles

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