Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2022 Jan 20

PMID 35049660

Authors

Evelina I Nikelshparg

Adil A Baizhumanov

Zhanna V Bochkova

Sergey M Novikov

Dmitry I Yakubovsky

Aleksey V Arsenin

Valentyn S Volkov

Eugene A Goodilin

Anna A Semenova

Olga Sosnovtseva

Georgy V Maksimov

Nadezda A Brazhe

Affiliations

Soon will be listed here.

Abstract

Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.

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