Suppression of RNA Editing by MiR-17 Inhibits the Stemness of Melanoma Stem Cells

Overview

Journal Mol Ther Nucleic Acids

Publisher Cell Press

Date 2022 Jan 17

PMID 35036056

Authors

Yu Zhang

Xiaoyuan Yang

Yalei Cui

Xiaobo Zhang

Affiliations

Soon will be listed here.

Abstract

More and more evidence suggests that microRNA (miRNA) and RNA editing play key roles in the development and progression of tumor. However, the influence of miRNA-mediated RNA editing on tumor stem cells remains unclear. In this study, the results demonstrated that miR-17, which was downregulated in melanoma stem cells, acted as a tumor inhibitor by suppressing the stemness of melanoma stem cells and promoting cell differentiation. MiR-17 targeted (adenosine deaminase acting on RNA 2), a gene encoding an editing enzyme required for the maintenance of melanoma stem cell stemness. In melanoma stem cells, ADAR2 was responsible for mRNA editing, which was able to increase the stability of mRNA. The and data demonstrated that mRNA editing upregulated the expressions of stemness and anti-apoptotic genes by activating Rac1 and then phosphorylating Akt and NF-κB, thus leading to oncogenesis of melanoma stem cells. Our findings contribute new perspectives to miRNA-regulated RNA editing in tumor progression.

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