Long Noncoding RNA ZFAS1 Protects HK-2 Cells Against Sepsis-Induced Injury Through Targeting the MiR3723p/PPAR Axis

Overview

Journal J Healthc Eng

Specialty Biomedical Engineering

Date 2022 Jan 17

PMID 35035856

Authors

Bingchang Hei

Caifang Yue

Yao Sun

Affiliations

Soon will be listed here.

Abstract

In septic acute kidney injury, one of the main purposes of long noncoding RNA (lncRNA) ZFAS1 is still unclear. This study is intended to analyze the effects of lncRNA ZFAS1 on the septic AKI in the HK-2 cell line. . In order to construct an in vitro model of septic AKI, HK-2 cells have been treated with lipopolysaccharides. CCK-8 assay has been utilized to check the viability of HK-2 cells. The contents of inflammatory cytokines (that includes IL-1, TNF-, and IL-6) have been marked with enzyme-linked immune sorbent assay (ELISA). Cell apoptosis was assessed by TUNEL staining. To detect the expression of lncRNA ZFAS1 and microRNA-372-3p, quantitative reverse-transcription PCR has been used. And to confirm the connection among genes, luciferase reporter assay has been applied. . Overexpression of ZFAS1 alleviated LPS-induced HK-2 cell injury. ZFAS1 positively regulated expression of receptor activated by peroxisome proliferation (PPAR) through competitive linkage with miR-372-3p. In addition, over expression of miR-372-3p counteracted the protective effect of upward regulation of ZFAS1 on LPS-induced HK-2 cell damage, which could be reversed by over expression of PPAR. . It is concluded that, in LPS-induced HK-2 cell injury, ZFAS1 has a protective role via modulating the miR-372-3p/PPAR axis, suggesting the potential of ZFAS1 as a protective target for septic AKI.

Citing Articles

Retracted: Long Noncoding RNA ZFAS1 Protects HK-2 Cells against Sepsis-Induced Injury through Targeting the miR3723p/PPAR Axis.

Healthcare Engineering J J Healthc Eng. 2023; 2023:9859657.

PMID: 37772014 PMC: 10533278. DOI: 10.1155/2023/9859657.

Influence of early enteral nutrition plus probiotics on intestinal function of senile patients with sepsis.

Xie H, Cai M, Zhang Y Am J Transl Res. 2023; 15(1):445-451.

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References

Tan J, Fan J, He J, Zhao L, Tang H . Knockdown of LncRNA DLX6-AS1 inhibits HK-2 cell pyroptosis via regulating miR-223-3p/NLRP3 pathway in lipopolysaccharide-induced acute kidney injury. J Bioenerg Biomembr. 2020; 52(5):367-376. DOI: 10.1007/s10863-020-09845-5. View

Fassan M, Baffa R, Palazzo J, Lloyd J, Crosariol M, Liu C . MicroRNA expression profiling of male breast cancer. Breast Cancer Res. 2009; 11(4):R58. PMC: 2750120. DOI: 10.1186/bcr2348. View

Zheng Y, Shi X, Hou J, Gao S, Chao Y, Ding J . Integrating metabolomics and network pharmacology to explore Rhizoma Coptidis extracts against sepsis-associated acute kidney injury. J Chromatogr B Analyt Technol Biomed Life Sci. 2021; 1164:122525. DOI: 10.1016/j.jchromb.2021.122525. View

Zhang X, Huang Z, Wang Y, Wang T, Li J, Xi P . Long Non-Coding RNA RMRP Contributes to Sepsis-Induced Acute Kidney Injury. Yonsei Med J. 2021; 62(3):262-273. PMC: 7934096. DOI: 10.3349/ymj.2021.62.3.262. View

Deng L, Wang Q, Yu C, Gao M . lncRNA PVT1 modulates NLRP3‑mediated pyroptosis in septic acute kidney injury by targeting miR‑20a‑5p. Mol Med Rep. 2021; 23(4). DOI: 10.3892/mmr.2021.11910. View

Dong D, Mu Z, Zhao C, Sun M . : a novel tumor-related long non-coding RNA. Cancer Cell Int. 2018; 18:125. PMC: 6122210. DOI: 10.1186/s12935-018-0623-y. View

Patschan D, Schwarze K, Henze E, Patschan S, Scheidemann R, Muller G . Fibrate treatment of eEOCs in murine AKI. J Nephrol. 2014; 27(1):37-44. DOI: 10.1007/s40620-013-0027-y. View

Fani F, Regolisti G, Delsante M, Cantaluppi V, Castellano G, Gesualdo L . Recent advances in the pathogenetic mechanisms of sepsis-associated acute kidney injury. J Nephrol. 2017; 31(3):351-359. DOI: 10.1007/s40620-017-0452-4. View

Chen Z . Progress and prospects of long noncoding RNAs in lipid homeostasis. Mol Metab. 2016; 5(3):164-170. PMC: 4770261. DOI: 10.1016/j.molmet.2015.12.003. View

10.

Zhao H, Chen B, Li Z, Wang B, Li L . Long Noncoding RNA DANCR Suppressed Lipopolysaccharide-Induced Septic Acute Kidney Injury by Regulating miR-214 in HK-2 Cells. Med Sci Monit. 2020; 26:e921822. PMC: 7139187. DOI: 10.12659/MSM.921822. View

11.

Lorenzen J, Kaucsar T, Schauerte C, Schmitt R, Rong S, Hubner A . MicroRNA-24 antagonism prevents renal ischemia reperfusion injury. J Am Soc Nephrol. 2014; 25(12):2717-29. PMC: 4243358. DOI: 10.1681/ASN.2013121329. View

12.

Fan J, Zhang J, Huang S, Li P . lncRNA OSER1-AS1 acts as a ceRNA to promote tumorigenesis in hepatocellular carcinoma by regulating miR-372-3p/Rab23 axis. Biochem Biophys Res Commun. 2019; 521(1):196-203. DOI: 10.1016/j.bbrc.2019.10.105. View

13.

Zhao M, Yang Y, Li J, Lu M, Wu Y . Silencing of OIP5-AS1 Protects Endothelial Cells From ox-LDL-Triggered Injury by Regulating KLF5 Expression via Sponging miR-135a-5p. Front Cardiovasc Med. 2021; 8:596506. PMC: 7994260. DOI: 10.3389/fcvm.2021.596506. View

14.

Wang Q, Liu S, Zhao X, Wang Y, Tian D, Jiang W . MiR-372-3p promotes cell growth and metastasis by targeting FGF9 in lung squamous cell carcinoma. Cancer Med. 2017; 6(6):1323-1330. PMC: 5463061. DOI: 10.1002/cam4.1026. View

15.

Szafranski P, Stankiewicz P . Long Non-Coding RNA : Gene Structure, Expression, and Biological Relevance. Genes (Basel). 2021; 12(2). PMC: 7911649. DOI: 10.3390/genes12020177. View

16.

Soliman M, Ragheb M, Elzayat E, Mohamed M, El-Ekiaby N, Abdelaziz A . MicroRNA-372-3p Predicts Response of TACE Patients Treated with Doxorubicin and Enhances Chemosensitivity in Hepatocellular Carcinoma. Anticancer Agents Med Chem. 2020; 21(2):246-253. DOI: 10.2174/1871520620666200516145830. View

17.

Fenner B, Darden D, Kelly L, Rincon J, Brakenridge S, Larson S . Immunological Endotyping of Chronic Critical Illness After Severe Sepsis. Front Med (Lausanne). 2021; 7:616694. PMC: 7917137. DOI: 10.3389/fmed.2020.616694. View

18.

Zhang Y, Zhang Y . lncRNA ZFAS1 Improves Neuronal Injury and Inhibits Inflammation, Oxidative Stress, and Apoptosis by Sponging miR-582 and Upregulating NOS3 Expression in Cerebral Ischemia/Reperfusion Injury. Inflammation. 2020; 43(4):1337-1350. DOI: 10.1007/s10753-020-01212-1. View

19.

Wang Y, Yang C, Zhang D, Chen Y, Yu X, Zhao Y . Long non-coding RNAs: A double-edged sword in aging kidney and renal disease. Chem Biol Interact. 2021; 337:109396. DOI: 10.1016/j.cbi.2021.109396. View

20.

Gomez H, Kellum J . Sepsis-induced acute kidney injury. Curr Opin Crit Care. 2016; 22(6):546-553. PMC: 5654474. DOI: 10.1097/MCC.0000000000000356. View