Single-chain Tandem Macrocyclic Peptides As a Scaffold for Growth Factor and Cytokine Mimetics

Overview

Journal Commun Biol

Specialty Biology

Date 2022 Jan 15

PMID 35031676

Authors

Kenichiro Ito

Yoshihiko Matsuda

Ayako Mine

Natsuki Shikida

Kazutoshi Takahashi

Kyohei Miyairi

Kazutaka Shimbo

Yoshimi Kikuchi

Atsushi Konishi

Affiliations

Soon will be listed here.

Abstract

Mimetics of growth factors and cytokines are promising tools for culturing large numbers of cells and manufacturing regenerative medicine products. In this study, we report single-chain tandem macrocyclic peptides (STaMPtides) as mimetics in a new multivalent peptide format. STaMPtides, which contain two or more macrocyclic peptides with a disulfide-closed backbone and peptide linkers, are successfully secreted into the supernatant by Corynebacterium glutamicum-based secretion technology. Without post-secretion modification steps, such as macrocyclization or enzymatic treatment, bacterially secreted STaMPtides form disulfide bonds, as designed; are biologically active; and show agonistic activities against respective target receptors. We also demonstrate, by cell-based assays, the potential of STaMPtides, which mimic growth factors and cytokines, in cell culture. The STaMPtide technology can be applied to the design, screening, and production of growth factor and cytokine mimetics.

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