FeO Nanoparticles Coated with Carboxymethyl Chitosan Containing Curcumin in Combination with Hyperthermia Induced Apoptosis in Breast Cancer Cells

Overview

Journal Prog Biomater

Specialty Biomedical Engineering

Date 2022 Jan 13

PMID 35025086

Authors

Negin Pazouki

Shiva Irani

Nafiseh Olov

Seyed Mohammad Atyabi

Shadab Bagheri-Khoulenjani

Affiliations

Soon will be listed here.

Abstract

Many studies have demonstrated that curcumin has potential anticancer properties. This research aims to study the effect of iron (II, III) oxide (FeO) nanoparticles coated with carboxymethyl chitosan containing curcumin combination with hyperthermia on breast cancer cells. Magnetic nanoparticles coated with carboxymethyl chitosan containing curcumin (MNP-CMC-CUR) were prepared and specified. MCF-7, MDA-MB-231, and human fibroblast cells were treated with free curcumin and MNP-CMC-CUR at concentrations of 0-60 µM and at different time points. A combined therapy of MNP-CMC-CUR and hyperthermia was performed on MCF-7 cells. The cytotoxicity of curcumin and MNP-CMC-CUR combined with hyperthermia was assessed by MTT. The changes in TP53 and CASPASE3 gene expression were evaluated using real-time PCR. Both cell apoptosis and cell cycle were studied by Annexin/PI staining. The results of MTT showed that the IC amount of MNP-CMC-CUR has significantly decreased compared to free curcumin (p < 0.05) and MNP-CMC-CUR in combination with the hyperthermia, and significantly reducing the metabolic activity of the cells (p < 0.05). Real-time PCR results revealed the up-regulation of TP53 and CASPASE3 (p < 0.05). The combinational therapy-induced cell apoptosis (64.51%) and sub-G1 cell cycle were arrested in MCF-7 cells. Based on these observations, a combination of MNP-CMC-CUR with hyperthermia could inhibit the proliferation of MCF-7 cells.

Citing Articles

Targeting STAT3 signaling pathway by curcumin and its analogues for breast cancer: A narrative review.

Golmohammadi M, Zamanian M, Al-Ani A, Jabbar T, Kareem A, Aghaei Z Animal Model Exp Med. 2024; 7(6):853-867.

PMID: 39219410 PMC: 11680487. DOI: 10.1002/ame2.12491.

A Comprehensive Review of Nanoparticles: From Classification to Application and Toxicity.

Eker F, Duman H, Akdasci E, Bolat E, Saritas S, Karav S Molecules. 2024; 29(15).

PMID: 39124888 PMC: 11314082. DOI: 10.3390/molecules29153482.

The Bright Side of Curcumin: A Narrative Review of Its Therapeutic Potential in Cancer Management.

Amaroli A, Panfoli I, Bozzo M, Ferrando S, Candiani S, Ravera S Cancers (Basel). 2024; 16(14).

PMID: 39061221 PMC: 11275093. DOI: 10.3390/cancers16142580.

Breast Cancer Treatment Using the Magneto-Hyperthermia Technique Associated with Omega-3 Polyunsaturated Fatty Acids' Supplementation and Physical Training.

Theinel M, Penteado Nucci M, Cianciarullo G, Oliveira F, Alves A, Mamani J Pharmaceutics. 2024; 16(3).

PMID: 38543205 PMC: 10976084. DOI: 10.3390/pharmaceutics16030310.

Antioxidant curcumin induces oxidative stress to kill tumor cells (Review).

Hu Y, Cheng L, Du S, Wang K, Liu S Oncol Lett. 2024; 27(2):67.

PMID: 38192657 PMC: 10773205. DOI: 10.3892/ol.2023.14200.

References

Hossen S, Hossain M, Basher M, Mia M, Rahman M, Uddin M . Smart nanocarrier-based drug delivery systems for cancer therapy and toxicity studies: A review. J Adv Res. 2018; 15:1-18. PMC: 6300464. DOI: 10.1016/j.jare.2018.06.005. View

Aiya Subramani P, Panati K, Narala V . Curcumin Nanotechnologies and Its Anticancer Activity. Nutr Cancer. 2017; 69(3):381-393. DOI: 10.1080/01635581.2017.1285405. View

Olanipekun E, Ayodele O, Olatunde O, Olusegun S . Comparative studies of chitosan and carboxymethyl chitosan doped with nickel and copper: Characterization and antibacterial potential. Int J Biol Macromol. 2021; 183:1971-1977. DOI: 10.1016/j.ijbiomac.2021.05.162. View

Minaei S, Khoei S, Khoee S, Vafashoar F, Pirhajati Mahabadi V . In vitro anti-cancer efficacy of multi-functionalized magnetite nanoparticles combining alternating magnetic hyperthermia in glioblastoma cancer cells. Mater Sci Eng C Mater Biol Appl. 2019; 101:575-587. DOI: 10.1016/j.msec.2019.04.007. View

Chang D, Lim M, Goos J, Qiao R, Ng Y, Mansfeld F . Biologically Targeted Magnetic Hyperthermia: Potential and Limitations. Front Pharmacol. 2018; 9:831. PMC: 6083434. DOI: 10.3389/fphar.2018.00831. View

Hou C, Lin F, Hou S, Liu J . Hyperthermia induces apoptosis through endoplasmic reticulum and reactive oxygen species in human osteosarcoma cells. Int J Mol Sci. 2014; 15(10):17380-95. PMC: 4227168. DOI: 10.3390/ijms151017380. View

Vakili-Ghartavol R, Momtazi-Borojeni A, Vakili-Ghartavol Z, Aiyelabegan H, Jaafari M, Rezayat S . Toxicity assessment of superparamagnetic iron oxide nanoparticles in different tissues. Artif Cells Nanomed Biotechnol. 2020; 48(1):443-451. DOI: 10.1080/21691401.2019.1709855. View

Manso J, Sharifi-Rad J, Zam W, Tsouh Fokou P, Martorell M, Pezzani R . Plant Natural Compounds in the Treatment of Adrenocortical Tumors. Int J Endocrinol. 2021; 2021:5516285. PMC: 8463247. DOI: 10.1155/2021/5516285. View

Zou Y, Liu P, Liu C, Zhi X . Doxorubicin-loaded mesoporous magnetic nanoparticles to induce apoptosis in breast cancer cells. Biomed Pharmacother. 2015; 69:355-60. DOI: 10.1016/j.biopha.2014.12.012. View

10.

Valencia O, Samuel S, Viscusi R, Riall T, Neumayer L, Aziz H . The Role of Genetic Testing in Patients With Breast Cancer: A Review. JAMA Surg. 2017; 152(6):589-594. DOI: 10.1001/jamasurg.2017.0552. View

11.

Shariatinia Z . Carboxymethyl chitosan: Properties and biomedical applications. Int J Biol Macromol. 2018; 120(Pt B):1406-1419. DOI: 10.1016/j.ijbiomac.2018.09.131. View

12.

Mansouri K, Rasoulpoor S, Daneshkhah A, Abolfathi S, Salari N, Mohammadi M . Clinical effects of curcumin in enhancing cancer therapy: A systematic review. BMC Cancer. 2020; 20(1):791. PMC: 7446227. DOI: 10.1186/s12885-020-07256-8. View

13.

Barahuie F, Dorniani D, Saifullah B, Gothai S, Hussein M, Pandurangan A . Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug-delivery system. Int J Nanomedicine. 2017; 12:2361-2372. PMC: 5376211. DOI: 10.2147/IJN.S126245. View

14.

Laffon B, Fernandez-Bertolez N, Costa C, Brandao F, Teixeira J, Pasaro E . Cellular and Molecular Toxicity of Iron Oxide Nanoparticles. Adv Exp Med Biol. 2018; 1048:199-213. DOI: 10.1007/978-3-319-72041-8_12. View

15.

Jose J, Kumar R, Harilal S, Mathew G, Parambi D, Prabhu A . Magnetic nanoparticles for hyperthermia in cancer treatment: an emerging tool. Environ Sci Pollut Res Int. 2019; 27(16):19214-19225. DOI: 10.1007/s11356-019-07231-2. View

16.

Espinosa A, Di Corato R, Kolosnjaj-Tabi J, Flaud P, Pellegrino T, Wilhelm C . Duality of Iron Oxide Nanoparticles in Cancer Therapy: Amplification of Heating Efficiency by Magnetic Hyperthermia and Photothermal Bimodal Treatment. ACS Nano. 2016; 10(2):2436-46. DOI: 10.1021/acsnano.5b07249. View

17.

Boice A, Bouchier-Hayes L . Targeting apoptotic caspases in cancer. Biochim Biophys Acta Mol Cell Res. 2020; 1867(6):118688. PMC: 7155770. DOI: 10.1016/j.bbamcr.2020.118688. View

18.

Salatin S, Dizaj S, Khosroushahi A . Effect of the surface modification, size, and shape on cellular uptake of nanoparticles. Cell Biol Int. 2015; 39(8):881-90. DOI: 10.1002/cbin.10459. View

19.

Rao W, Zhang W, Poventud-Fuentes I, Wang Y, Lei Y, Agarwal P . Thermally responsive nanoparticle-encapsulated curcumin and its combination with mild hyperthermia for enhanced cancer cell destruction. Acta Biomater. 2014; 10(2):831-42. PMC: 4136765. DOI: 10.1016/j.actbio.2013.10.020. View

20.

Zhou X, Hao Q, Lu H . Mutant p53 in cancer therapy-the barrier or the path. J Mol Cell Biol. 2018; 11(4):293-305. PMC: 6487791. DOI: 10.1093/jmcb/mjy072. View