ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Jan 11

PMID 35011634

Authors

Denise Eckert

Felicitas Rapp

Ayele T Tsedeke

Jessica Molendowska

Robert Lehn

Markus Langhans

Claudia Fournier

Franz Rodel

Stephanie Hehlgans

Affiliations

Soon will be listed here.

Abstract

Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose-effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0-2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.

Citing Articles

NADPH Oxidases in Cancer Therapy-Induced Cardiotoxicity: Mechanisms and Therapeutic Approaches.

Adhab A, Altalbawy F, Mahdi M, Baldaniya L, Omar T, Ganesan S Cardiovasc Toxicol. 2025; 25(4):631-649.

PMID: 39966326 DOI: 10.1007/s12012-025-09976-4.

Characterization of the phagocytic ability of white blood cells separated using a single curvature spiral microfluidic device.

Mane S, Jacob P, Hemadri V, Dey P, Bhand S, Tripathi S Biomed Eng Lett. 2024; 14(6):1409-1419.

PMID: 39465117 PMC: 11502713. DOI: 10.1007/s13534-024-00414-y.

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.

Mohammadzadeh Boukani L, Ezzati M, Ferdowsi Khosroshahi A, Kheirjou R Cell Tissue Bank. 2024; 25(3):785-804.

PMID: 38869670 DOI: 10.1007/s10561-024-10143-2.

ROS regulation in gliomas: implications for treatment strategies.

Yang Y, Zhu Y, Sun S, Zhao C, Bai Y, Wang J Front Immunol. 2023; 14:1259797.

PMID: 38130720 PMC: 10733468. DOI: 10.3389/fimmu.2023.1259797.

Bacterial lipopolysaccharide-induced endothelial activation and dysfunction: a new predictive and therapeutic paradigm for sepsis.

Wang M, Feng J, Zhou D, Wang J Eur J Med Res. 2023; 28(1):339.

PMID: 37700349 PMC: 10498524. DOI: 10.1186/s40001-023-01301-5.

References

El Assar M, Angulo J, Rodriguez-Manas L . Oxidative stress and vascular inflammation in aging. Free Radic Biol Med. 2013; 65:380-401. DOI: 10.1016/j.freeradbiomed.2013.07.003. View

Lapytsko A, Kollarovic G, Ivanova L, Studencka M, Schaber J . FoCo: a simple and robust quantification algorithm of nuclear foci. BMC Bioinformatics. 2015; 16:392. PMC: 4654864. DOI: 10.1186/s12859-015-0816-5. View

Chen B, Lu Y, Chen Y, Cheng J . The role of Nrf2 in oxidative stress-induced endothelial injuries. J Endocrinol. 2015; 225(3):R83-99. DOI: 10.1530/JOE-14-0662. View

Cucu A, Shreder K, Kraft D, Ruhle P, Klein G, Thiel G . Decrease of Markers Related to Bone Erosion in Serum of Patients with Musculoskeletal Disorders after Serial Low-Dose Radon Spa Therapy. Front Immunol. 2017; 8:882. PMC: 5524779. DOI: 10.3389/fimmu.2017.00882. View

Minten M, Mahler E, den Broeder A, Leer J, van den Ende C . The efficacy and safety of low-dose radiotherapy on pain and functioning in patients with osteoarthritis: a systematic review. Rheumatol Int. 2016; 36(1):133-42. DOI: 10.1007/s00296-015-3337-7. View

Prescott S, McIntyre T, Zimmerman G . Events at the vascular wall: the molecular basis of inflammation. J Investig Med. 2001; 49(1):104-11. DOI: 10.2310/6650.2001.34106. View

Matlung H, Bakker E, VanBavel E . Shear stress, reactive oxygen species, and arterial structure and function. Antioxid Redox Signal. 2009; 11(7):1699-709. DOI: 10.1089/ars.2008.2408. View

Hsieh H, Liu C, Huang B, Tseng A, Wang D . Shear-induced endothelial mechanotransduction: the interplay between reactive oxygen species (ROS) and nitric oxide (NO) and the pathophysiological implications. J Biomed Sci. 2014; 21:3. PMC: 3898375. DOI: 10.1186/1423-0127-21-3. View

Erbeldinger N, Rapp F, Ktitareva S, Wendel P, Bothe A, Dettmering T . Measuring Leukocyte Adhesion to (Primary) Endothelial Cells after Photon and Charged Particle Exposure with a Dedicated Laminar Flow Chamber. Front Immunol. 2017; 8:627. PMC: 5451490. DOI: 10.3389/fimmu.2017.00627. View

10.

Lumniczky K, Impens N, Armengol G, Candeias S, Georgakilas A, Hornhardt S . Low dose ionizing radiation effects on the immune system. Environ Int. 2020; 149:106212. PMC: 8784945. DOI: 10.1016/j.envint.2020.106212. View

11.

Scioli M, Bielli A, Agostinelli S, Tarquini C, Arcuri G, Ferlosio A . Antioxidant treatment prevents serum deprivation- and TNF-α-induced endothelial dysfunction through the inhibition of NADPH oxidase 4 and the restoration of β-oxidation. J Vasc Res. 2014; 51(5):327-37. DOI: 10.1159/000365926. View

12.

Craige S, Kant S, Keaney Jr J . Reactive oxygen species in endothelial function - from disease to adaptation - . Circ J. 2015; 79(6):1145-55. DOI: 10.1253/circj.CJ-15-0464. View

13.

Pi X, Xie L, Patterson C . Emerging Roles of Vascular Endothelium in Metabolic Homeostasis. Circ Res. 2018; 123(4):477-494. PMC: 6205216. DOI: 10.1161/CIRCRESAHA.118.313237. View

14.

Chistiakov D, Orekhov A, Bobryshev Y . Effects of shear stress on endothelial cells: go with the flow. Acta Physiol (Oxf). 2016; 219(2):382-408. DOI: 10.1111/apha.12725. View

15.

Ray P, Huang B, Tsuji Y . Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 2012; 24(5):981-90. PMC: 3454471. DOI: 10.1016/j.cellsig.2012.01.008. View

16.

Deem T, Cook-Mills J . Vascular cell adhesion molecule 1 (VCAM-1) activation of endothelial cell matrix metalloproteinases: role of reactive oxygen species. Blood. 2004; 104(8):2385-93. PMC: 2710031. DOI: 10.1182/blood-2004-02-0665. View

17.

Burmester G, Bijlsma J, Cutolo M, McInnes I . Managing rheumatic and musculoskeletal diseases - past, present and future. Nat Rev Rheumatol. 2017; 13(7):443-448. DOI: 10.1038/nrrheum.2017.95. View

18.

Abbasi M, Mousavi M, Jamalzehi S, Alimohammadi R, Bezvan M, Mohammadi H . Strategies toward rheumatoid arthritis therapy; the old and the new. J Cell Physiol. 2018; 234(7):10018-10031. DOI: 10.1002/jcp.27860. View

19.

Tang C . Research of Pathogenesis and Novel Therapeutics in Arthritis. Int J Mol Sci. 2019; 20(7). PMC: 6479975. DOI: 10.3390/ijms20071646. View

20.

Arenas M, Gil F, Gironella M, Hernandez V, Jorcano S, Biete A . Anti-inflammatory effects of low-dose radiotherapy in an experimental model of systemic inflammation in mice. Int J Radiat Oncol Biol Phys. 2006; 66(2):560-7. DOI: 10.1016/j.ijrobp.2006.06.004. View