A Sensitive Capacitive Biosensor for Protein a Detection Using Human IgG Immobilized on an Electrode Using Layer-by-Layer Applied Gold Nanoparticles

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2022 Jan 11

PMID 35009642

Authors

Kosin Teeparuksapun

Martin Hedstrom

Bo Mattiasson

Affiliations

Soon will be listed here.

Abstract

A capacitive biosensor for the detection of protein A was developed. Gold electrodes were fabricated by thermal evaporation and patterned by photoresist photolithography. A layer-by-layer (LbL) assembly of thiourea (TU) and HAuCl and chemical reduction was utilized to prepare a probe with a different number of layers of TU and gold nanoparticles (AuNPs). The LbL-modified electrodes were used for the immobilization of human IgG. The binding interaction between human IgG and protein A was detected as a decrease in capacitance signal, and that change was used to investigate the correlation between the height of the LbL probe and the sensitivity of the capacitive measurement. The results showed that the initial increase in length of the LbL probe can enhance the amount of immobilized human IgG, leading to a more sensitive assay. However, with thicker LbL layers, a reduction of the sensitivity of the measurement was registered. The performance of the developed system under optimum set-up showed a linearity in response from 1 × 10 to 1 × 10 M, with the limit detection of 9.1 × 10 M, which could be interesting for the detection of trace amounts of protein A from affinity isolation of therapeutic monoclonal antibodies.

Citing Articles

Parkinson biomarker determination with an Au microflower-enhanced electrochemical immunosensor using non-Faradaic capacitance measurements.

Orzari L, Brazaca L, Janegitz B Mikrochim Acta. 2024; 191(11):663.

PMID: 39392501 DOI: 10.1007/s00604-024-06747-w.

Capacitive immunosensor for COVID-19 diagnosis.

Sampaio I, Takeuti N, Gusson B, Machado T, Zucolotto V Microelectron Eng. 2022; 267:111912.

PMID: 36406866 PMC: 9643278. DOI: 10.1016/j.mee.2022.111912.

References

Tang D, Yuan R, Chai Y . Quartz crystal microbalance immunoassay for carcinoma antigen 125 based on gold nanowire-functionalized biomimetic interface. Analyst. 2008; 133(7):933-8. DOI: 10.1039/b719389k. View

Labib M, Hedstrom M, Amin M, Mattiasson B . A capacitive immunosensor for detection of cholera toxin. Anal Chim Acta. 2009; 634(2):255-61. DOI: 10.1016/j.aca.2008.12.035. View

Berggren C, Johansson G . Capacitance measurements of antibody-antigen interactions in a flow system. Anal Chem. 1997; 69(18):3651-7. DOI: 10.1021/ac970203e. View

Schenauer M, Flynn G, Goetze A . Identification and quantification of host cell protein impurities in biotherapeutics using mass spectrometry. Anal Biochem. 2012; 428(2):150-7. DOI: 10.1016/j.ab.2012.05.018. View

Rey G, Wendeler M . Full automation and validation of a flexible ELISA platform for host cell protein and protein A impurity detection in biopharmaceuticals. J Pharm Biomed Anal. 2012; 70:580-6. DOI: 10.1016/j.jpba.2012.05.027. View

Gomez M, Lee A, Reddy B, Muir A, Soong G, Pitt A . Staphylococcus aureus protein A induces airway epithelial inflammatory responses by activating TNFR1. Nat Med. 2004; 10(8):842-8. DOI: 10.1038/nm1079. View

Gilgunn S, El-Sabbahy H, Albrecht S, Gaikwad M, Corrigan K, Deakin L . Identification and tracking of problematic host cell proteins removed by a synthetic, highly functionalized nonwoven media in downstream bioprocessing of monoclonal antibodies. J Chromatogr A. 2019; 1595:28-38. DOI: 10.1016/j.chroma.2019.02.056. View

Machado G, Feil A, Migowski P, Rossi L, Giovanela M, Crespo J . Structural control of gold nanoparticles self-assemblies by layer-by-layer process. Nanoscale. 2011; 3(4):1717-23. DOI: 10.1039/c0nr01023e. View

Kang J, Choi H, Hwang S, Han S, Jeon J, Lee E . Improving immunobinding using oriented immobilization of an oxidized antibody. J Chromatogr A. 2007; 1161(1-2):9-14. DOI: 10.1016/j.chroma.2007.05.023. View

10.

Dawan S, Kanatharana P, Wongkittisuksa B, Limbut W, Numnuam A, Limsakul C . Label-free capacitive immunosensors for ultra-trace detection based on the increase of immobilized antibodies on silver nanoparticles. Anal Chim Acta. 2011; 699(2):232-41. DOI: 10.1016/j.aca.2011.05.038. View

11.

Gupta S, Jain U, Murti B, Putri A, Tiwari A, Chauhan N . Nanohybrid-based immunosensor prepared for Helicobacter pylori BabA antigen detection through immobilized antibody assembly with @ Pd/rGO/PEDOT sensing platform. Sci Rep. 2020; 10(1):21217. PMC: 7719176. DOI: 10.1038/s41598-020-78068-w. View

12.

Berggren C, Bjarnason B, Johansson G . An immunological interleukine-6 capacitive biosensor using perturbation with a potentiostatic step. Biosens Bioelectron. 1998; 13(10):1061-8. DOI: 10.1016/s0956-5663(98)00058-x. View

13.

Zhu-Shimoni J, Gunawan F, Thomas A, Vanderlaan M, Stults J . Trace level analysis of leached Protein A in bioprocess samples without interference from the large excess of rhMAb IgG. J Immunol Methods. 2008; 341(1-2):59-67. DOI: 10.1016/j.jim.2008.10.015. View

14.

Niyomdecha S, Limbut W, Numnuam A, Kanatharana P, Charlermroj R, Karoonuthaisiri N . Phage-based capacitive biosensor for Salmonella detection. Talanta. 2018; 188:658-664. DOI: 10.1016/j.talanta.2018.06.033. View

15.

Bertram J, Grunberg S, Shulman I, Apuzzo M, Boquiren D, Kunkel L . Staphylococcal Protein A column: correlation of mitogenicity of perfused plasma with clinical response. Cancer Res. 1985; 45(9):4486-94. View

16.

Wu H, Liu Z, Wang X, Zhao B, Zhang J, Li C . Preparation of hollow capsule-stabilized gold nanoparticles through the encapsulation of the dendrimer. J Colloid Interface Sci. 2006; 302(1):142-8. DOI: 10.1016/j.jcis.2006.06.019. View

17.

Teeparuksapun K, Hedstrom M, Wong E, Tang S, Hewlett I, Mattiasson B . Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor. Anal Chem. 2010; 82(20):8406-11. DOI: 10.1021/ac102144a. View

18.

Teeparuksapun K, Hedstrom M, Kanatharana P, Thavarungkul P, Mattiasson B . Capacitive immunosensor for the detection of host cell proteins. J Biotechnol. 2011; 157(1):207-13. DOI: 10.1016/j.jbiotec.2011.11.004. View

19.

Mattiasson B, Teeparuksapun K, Hedstrom M . Immunochemical binding assays for detection and quantification of trace impurities in biotechnological production. Trends Biotechnol. 2009; 28(1):20-7. DOI: 10.1016/j.tibtech.2009.10.002. View

20.

Chen S, Chuang Y, Lu Y, Lin H, Yang Y, Lin C . A method of layer-by-layer gold nanoparticle hybridization in a quartz crystal microbalance DNA sensing system used to detect dengue virus. Nanotechnology. 2009; 20(21):215501. DOI: 10.1088/0957-4484/20/21/215501. View