Control of Thousand-Grain Weight by in Rice

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jan 11

PMID 35008551

Authors

Zi-Wei Zuo

Zhen-Hua Zhang

De-Run Huang

Ye-Yang Fan

Si-Bin Yu

Jie-Yun Zhuang

Yu-Jun Zhu

Affiliations

Soon will be listed here.

Abstract

Grain weight and size are important traits determining grain yield and influencing grain quality in rice. In a previous study, a quantitative trait locus controlling thousand-grain weight (TGW) in rice, , was mapped in a 70.7 kb region on chromosome 10. Validation of the candidate gene for , encoding a MADS-box transcription factor, was performed in this study. In a near-isogenic line (NIL) population segregated only at the locus, NILs carrying the allele of IRBB52 were 1.9% and 2.9% lower in TGW than NILs carrying the allele of Teqing in 2018 and 2020, respectively. Using knock-out mutants and overexpression transgenic plants, was validated as the causal gene for . Compared with the recipients, the TGW of the knock-out mutants was reduced by 6.0-15.0%. In these populations, decreased grain weight and size were associated with a reduction in the expression of . In transgenic populations of driven by a strong constitutive promoter, grain weight and size of the positive plants were significantly higher than those of the negative plants. Haplotype analysis showed that the Teqing-type allele of is the major type presented in cultivated rice and used in variety improvement. Cloning of provides a new gene resource to improve grain weight and size through molecular design breeding.

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