Real-time Vascular and IgA Dynamics During Henoch-Schönlein Purpura by Fluorescent Microscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 Jan 10

PMID 35003869

Authors

Yujie Zhu

Shaoyang Wang

Hui Xu

Hao He

Meng Pan

Affiliations

Soon will be listed here.

Abstract

Henoch-Schönlein purpura (HSP) is a typical cutaneous immune skin disease, usually diagnosed by invasive biopsy. In this study, we develop a noninvasive optical method by combining optical clearing, confocal microscopy and immune-staining together to present the real-time in vivo dynamics of blood vessels, IgA molecules, and T cells in a HSP rat model. The small vessels in the skin are found with acute damage and then hyperplasia, which enhances deposition of IgA complexes in blood vessels. The migrating T cells in blood vessels in HSP regions can be detected by setting fast line scanning in this method. Our method provides vascular, cellular, and molecular dynamics during HSP development and is thus of great potential in research and diagnosis of HSP and other skin diseases.

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