Three-dimensional Virtual Histology of the Cerebral Cortex Based on Phase-contrast X-ray Tomography

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 Jan 10

PMID 35003854

Authors

Marina Eckermann

Franziska van der Meer

Peter Cloetens

Torben Ruhwedel

Wiebke Mobius

Christine Stadelmann

Tim Salditt

Affiliations

Soon will be listed here.

Abstract

In this work, we optimize the setups and experimental parameters of X-ray phase-contrast computed-tomography for the three-dimensional imaging of the cyto- and myeloarchitecture of cerebral cortex, including both human and murine tissue. We present examples for different optical configurations using state-of-the art synchrotron instruments for holographic tomography, as well as compact laboratory setups for phase-contrast tomography in the direct contrast (edge-enhancement) regime. Apart from unstained and paraffin-embedded tissue, we tested hydrated tissue, as well as heavy metal stained and resin-embedded tissue using two different protocols. Further, we show that the image quality achieved allows to assess the neuropathology of multiple sclerosis in a biopsy sample collected during surgery.

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