Lipoic Acid Does Not Affect The Growth of Cells

Overview

Journal Pol J Microbiol

Specialty Microbiology

Date 2022 Jan 10

PMID 35003281

Authors

Malgorzata Biernat-Sudolska

Danuta Rojek-Zakrzewska

Paulina Gajda

Anna Bilska-Wilkosz

Affiliations

Soon will be listed here.

Abstract

is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some Archea. Research of recent years has increasingly pointed to the therapeutic properties of exogenously supplemented LA. The present study was conducted on 40 strains of cultured with the following LA concentrations: 1,200 μg/ml, 120 μg/ml, and 12 μg/ml. The bacterial colonies of each strain were counted and expressed as the number of colony-forming units/ml (CFU). The number of CFU in strains obtained in the presence of LA was compared with the number of CFU in the strains grown in the media without LA. The obtained results indicated that the presence of LA in the medium did not affect the growth of . The investigation of the influence of LA on the growth and survival of microbial cells not only allows for obtaining an answer to the question of whether LA has antimicrobial activity and, therefore, can be used as a drug supporting the treatment of patients infected with a given pathogenic microorganism. Such studies are also crucial for a better understanding of LA metabolism in the microbial cells, which is also important for the search for new antimicrobial drugs. This research is, therefore, an introduction to such further studies.

References

Bergin S, Mendis S, Young B, Binti Izharuddin E . Postoperative Mycoplasma hominis brain abscess: keep it in mind!. BMJ Case Rep. 2017; 2017. PMC: 5255544. DOI: 10.1136/bcr-2016-218022. View

Iciek M, Gorny M, Bilska-Wilkosz A, Kowalczyk-Pachel D . Is aldehyde dehydrogenase inhibited by sulfur compounds? In vitro and in vivo studies. Acta Biochim Pol. 2018; 65(1):125-132. DOI: 10.18388/abp.2017_2324. View

Meyer R, Clough W . Extragenital Mycoplasma hominis infections in adults: emphasis on immunosuppression. Clin Infect Dis. 1993; 17 Suppl 1:S243-9. DOI: 10.1093/clinids/17.supplement_1.s243. View

Ran M, Wang T, Shao M, Chen Z, Liu H, Xia Y . Sensitive Method for Reliable Quantification of Sulfane Sulfur in Biological Samples. Anal Chem. 2019; 91(18):11981-11986. DOI: 10.1021/acs.analchem.9b02875. View

Biernat-Sudolska M, Rojek-Zakrzewska D, Lauterbach R . Assessment of various diagnostic methods of ureaplasma respiratory tract infections in newborns. Acta Biochim Pol. 2006; 53(3):609-11. View

RODWELL A . A defined medium for Mycoplasma strain Y. J Gen Microbiol. 1969; 58(1):39-47. DOI: 10.1099/00221287-58-1-39. View

Qiu H, Lu W, Li M, Wang Z, Du Q, Wang A . The infection of Mycoplasma hominis after total knee replacement: Case report and literature review. Chin J Traumatol. 2017; 20(4):243-245. PMC: 5555244. DOI: 10.1016/j.cjtee.2017.04.005. View

Zhou M, Wang P, Chen S, Du B, Du J, Wang F . Meningitis in a Chinese adult patient caused by Mycoplasma hominis: a rare infection and literature review. BMC Infect Dis. 2016; 16(1):557. PMC: 5059901. DOI: 10.1186/s12879-016-1885-4. View

Krijnen M, Hekker T, Algra J, Wuisman P, van Royen B . Mycoplasma hominis deep wound infection after neuromuscular scoliosis surgery: the use of real-time polymerase chain reaction (PCR). Eur Spine J. 2006; 15 Suppl 5:599-603. PMC: 1602191. DOI: 10.1007/s00586-005-0055-y. View

10.

Koch A, Bilina A, Teodorowicz L, Stary A . Mycoplasma hominis and Ureaplasma urealyticum in patients with sexually transmitted diseases. Wien Klin Wochenschr. 1997; 109(14-15):584-9. View

11.

Whitson W, Ball P, Lollis S, Balkman J, Bauer D . Postoperative Mycoplasma hominis infections after neurosurgical intervention. J Neurosurg Pediatr. 2014; 14(2):212-8. DOI: 10.3171/2014.4.PEDS13547. View

12.

Zhao G, Hu C, Xue Y . In vitro evaluation of chitosan-coated liposome containing both coenzyme Q10 and alpha-lipoic acid: Cytotoxicity, antioxidant activity, and antimicrobial activity. J Cosmet Dermatol. 2017; 17(2):258-262. DOI: 10.1111/jocd.12369. View

13.

Waites K, Schelonka R, Xiao L, Grigsby P, Novy M . Congenital and opportunistic infections: Ureaplasma species and Mycoplasma hominis. Semin Fetal Neonatal Med. 2008; 14(4):190-9. DOI: 10.1016/j.siny.2008.11.009. View

14.

Hou N, Yan Z, Fan K, Li H, Zhao R, Xia Y . OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli. Redox Biol. 2019; 26:101293. PMC: 6831875. DOI: 10.1016/j.redox.2019.101293. View

15.

Bilska-Wilkosz A, Iciek M, Kowalczyk-Pachel D, Gorny M, Sokolowska-Jezewicz M, Wlodek L . Lipoic Acid as a Possible Pharmacological Source of Hydrogen Sulfide/Sulfane Sulfur. Molecules. 2017; 22(3). PMC: 6155427. DOI: 10.3390/molecules22030388. View

16.

Miranda C, Camacho E, Reina G, Turino J, Rodriguez-Granger J, Yeste R . Isolation of Mycoplasma hominis from extragenital cultures. Eur J Clin Microbiol Infect Dis. 2005; 24(5):334-7. DOI: 10.1007/s10096-005-1326-6. View

17.

Zorzoli A, Grayczyk J, Alonzo 3rd F . Staphylococcus aureus Tissue Infection During Sepsis Is Supported by Differential Use of Bacterial or Host-Derived Lipoic Acid. PLoS Pathog. 2016; 12(10):e1005933. PMC: 5049849. DOI: 10.1371/journal.ppat.1005933. View

18.

Reed L, Debusk B, GUNSALUS I, HORNBERGER Jr C . Crystalline alpha-lipoic acid; a catalytic agent associated with pyruvate dehydrogenase. Science. 1951; 114(2952):93-4. DOI: 10.1126/science.114.2952.93. View

19.

Li W, Ma Y, Shi Q, Xie X, Sun T, Peng H . Diallyl disulfide from garlic oil inhibits Pseudomonas aeruginosa virulence factors by inactivating key quorum sensing genes. Appl Microbiol Biotechnol. 2018; 102(17):7555-7564. DOI: 10.1007/s00253-018-9175-2. View

20.

Luki N, Lebel P, Boucher M, Doray B, Turgeon J, Brousseau R . Comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. Eur J Clin Microbiol Infect Dis. 1998; 17(4):255-63. DOI: 10.1007/BF01699982. View