An Investigation on Fermentative Profile, Microbial Numbers, Bacterial Community Diversity and Their Predicted Metabolic Characteristics of Sudangrass (Sorghum Sudanense Stapf.) Silages

Overview

Journal Anim Biosci

Specialty Veterinary Medicine

Date 2022 Jan 6

PMID 34991212

Citations 2

Authors

Siran Wang

Junfeng Li

Jie Zhao

Zhihao Dong

Tao Shao

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the fermentation profiles, bacterial community and predicted metabolic characteristics of Sudangrass (Sorghum sudanense Stapf.) during ensiling.

Methods: First-cutting Sudangrass was harvested at the vegetative stage and ensiled in laboratory-scale silos (1 L capacity). Triplicate silos were sampled after 1, 3, 7, 15, 30, and 60 days of ensiling, respectively. The bacterial communities on day 3 and 60 were assessed through high-throughput sequencing technology, and 16S rRNA-gene predicted functional profiles were analyzed according to the Kyoto encyclopedia of genes and genomes using Tax4Fun.

Results: The Sudangrass silages showed good fermentation quality, indicated by higher lactic acid contents, and lower pH, butyric acid and ammonia nitrogen contents. The dominant genus Lactococcus on day 3 was replaced by Lactobacillus on day 60. The metabolism of amino acid, energy, cofactors and vitamins was restricted, and metabolism of nucleotide and carbohydrate was promoted after ensiling. The 1-phosphofructokinase and pyruvate kinase of bacterial community seemed to play important roles in stimulating the lactic acid fermentation, and the promotion of arginine deiminase could help lactic acid bacteria to tolerate the acidic environment.

Conclusion: High-throughput sequencing technology combined with 16S rRNA genepredicted functional analyses revealed the differences during the early and late stages of Sudangrass ensiling not only for distinct bacterial community but also for specific functional metabolites. The results could provide a comprehensive insight into bacterial community and metabolic characteristics to further improve the silage quality.

Citing Articles

Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage.

Zhao M, Zhang H, Pan G, Yin H, Sun J, Yu Z Front Microbiol. 2022; 13:1052837.

PMID: 36386706 PMC: 9664940. DOI: 10.3389/fmicb.2022.1052837.

Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage.

Fu Z, Sun L, Hou M, Hao J, Lu Q, Liu T Front Microbiol. 2022; 13:971449.

PMID: 36110305 PMC: 9468666. DOI: 10.3389/fmicb.2022.971449.

References

Asshauer K, Wemheuer B, Daniel R, Meinicke P . Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data. Bioinformatics. 2015; 31(17):2882-4. PMC: 4547618. DOI: 10.1093/bioinformatics/btv287. View

Bai J, Ding Z, Ke W, Xu D, Wang M, Huang W . Different lactic acid bacteria and their combinations regulated the fermentation process of ensiled alfalfa: ensiling characteristics, dynamics of bacterial community and their functional shifts. Microb Biotechnol. 2021; 14(3):1171-1182. PMC: 8085944. DOI: 10.1111/1751-7915.13785. View

Ali N, Wang S, Zhao J, Dong Z, Li J, Nazar M . Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota. Bioresour Technol. 2020; 314:123606. DOI: 10.1016/j.biortech.2020.123606. View

Gharechahi J, Kharazian Z, Sarikhan S, Salehi Jouzani G, Aghdasi M, Hosseini Salekdeh G . The dynamics of the bacterial communities developed in maize silage. Microb Biotechnol. 2017; 10(6):1663-1676. PMC: 5658587. DOI: 10.1111/1751-7915.12751. View

Wang Y, He L, Xing Y, Zhou W, Pian R, Yang F . Bacterial diversity and fermentation quality of Moringa oleifera leaves silage prepared with lactic acid bacteria inoculants and stored at different temperatures. Bioresour Technol. 2019; 284:349-358. DOI: 10.1016/j.biortech.2019.03.139. View

Ogunade I, Jiang Y, Pech Cervantes A, Kim D, Oliveira A, Vyas D . Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives. J Dairy Sci. 2017; 101(3):2048-2059. DOI: 10.3168/jds.2017-12876. View

Silva V, Pereira O, Leandro E, Silva T, Ribeiro K, Mantovani H . Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions. J Dairy Sci. 2016; 99(3):1895-1902. DOI: 10.3168/jds.2015-9792. View

Kilstrup M, Hammer K, Jensen P, Martinussen J . Nucleotide metabolism and its control in lactic acid bacteria. FEMS Microbiol Rev. 2005; 29(3):555-90. DOI: 10.1016/j.femsre.2005.04.006. View

Flythe M, Russell J . The effect of pH and a bacteriocin (bovicin HC5) on Clostridium sporogenes MD1, a bacterium that has the ability to degrade amino acids in ensiled plant materials. FEMS Microbiol Ecol. 2009; 47(2):215-22. DOI: 10.1016/S0168-6496(03)00259-9. View

10.

Yan Y, Li X, Guan H, Huang L, Ma X, Peng Y . Microbial community and fermentation characteristic of Italian ryegrass silage prepared with corn stover and lactic acid bacteria. Bioresour Technol. 2019; 279:166-173. DOI: 10.1016/j.biortech.2019.01.107. View

11.

Zheng M, Niu D, Jiang D, Zuo S, Xu C . Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar. J Appl Microbiol. 2017; 122(6):1456-1470. DOI: 10.1111/jam.13456. View

12.

Xu D, Wang N, Rinne M, Ke W, Weinberg Z, Da M . The bacterial community and metabolome dynamics and their interactions modulate fermentation process of whole crop corn silage prepared with or without inoculants. Microb Biotechnol. 2020; 14(2):561-576. PMC: 7936295. DOI: 10.1111/1751-7915.13623. View

13.

Van Soest P, Robertson J, Lewis B . Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991; 74(10):3583-97. DOI: 10.3168/jds.S0022-0302(91)78551-2. View

14.

Pang H, Tan Z, Qin G, Wang Y, Li Z, Jin Q . Phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the Tibetan Plateau. J Microbiol. 2012; 50(1):63-71. DOI: 10.1007/s12275-012-1284-5. View

15.

Wang Y, He L, Xing Y, Zheng Y, Zhou W, Pian R . Dynamics of Bacterial Community and Fermentation Quality during Ensiling of Wilted and Unwilted Leaf Silage with or without Lactic Acid Bacterial Inoculants. mSphere. 2019; 4(4). PMC: 6686226. DOI: 10.1128/mSphere.00341-19. View

16.

Ni K, Minh T, Tu T, Tsuruta T, Pang H, Nishino N . Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing. Appl Microbiol Biotechnol. 2016; 101(4):1385-1394. DOI: 10.1007/s00253-016-7900-2. View

17.

Ventura M, Canchaya C, Tauch A, Chandra G, Fitzgerald G, Chater K . Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. Microbiol Mol Biol Rev. 2007; 71(3):495-548. PMC: 2168647. DOI: 10.1128/MMBR.00005-07. View

18.

Broderick G, Kang J . Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci. 1980; 63(1):64-75. DOI: 10.3168/jds.S0022-0302(80)82888-8. View

19.

Hu Z, Niu H, Tong Q, Chang J, Yu J, Li S . The Microbiota Dynamics of Alfalfa Silage During Ensiling and After Air Exposure, and the Metabolomics After Air Exposure Are Affected by and Cellulase Addition. Front Microbiol. 2020; 11:519121. PMC: 7732661. DOI: 10.3389/fmicb.2020.519121. View

20.

Guan H, Yan Y, Li X, Li X, Shuai Y, Feng G . Microbial communities and natural fermentation of corn silages prepared with farm bunker-silo in Southwest China. Bioresour Technol. 2018; 265:282-290. DOI: 10.1016/j.biortech.2018.06.018. View