CmPMRl and CmPMrs Are Responsible for Resistance to Powdery Mildew Caused by Podosphaera Xanthii Race 1 in Melon

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2022 Jan 6

PMID 34989827

Citations 11

Authors

Haonan Cui

Chao Fan

Zhuo Ding

Xuezheng Wang

Lili Tang

Yingdong Bi

Feishi Luan

Peng Gao

Affiliations

Soon will be listed here.

Abstract

Two genes for resistance to Podosphaera xanthii race 1 in melon were identified on chromosomes 10 and 12 of the Cucumis melo cultivar MR-1. Cucumis melo L. is an economically important crop, the production of which is threatened by the prevalence of melon powdery mildew (PM) infections. We herein utilized the MR-1 (P; resistant to PM) and M4-7 (P; susceptible to PM) accessions to assess the heritability of PM (race 1) resistance in these melon plants. PM resistance in MR-1 leaves was linked to a dominant gene (CmPMRl), whereas stem resistance was under the control of a recessive gene (CmPMrs), with the dominant gene having an epistatic effect on the recessive gene. The CmPMRl gene was mapped to a 50 Kb interval on chromosome 12, while CmPMrs was mapped to an 89 Kb interval on chromosome 10. The CmPMRl candidate gene MELO3C002441 and the CmPMrs candidate gene MELO3C012438 were identified through sequence alignment, functional annotation, and expression pattern analyzes of all genes within these respective intervals. MELO3C002441 and MELO3C012438 were both localized to the cellular membrane and were contained conserved NPR gene-like and MLO domains, respectively, which were linked to PM resistance. In summary, we identified patterns of PM resistance in the disease-resistant MR-1 melon cultivar and identified two putative genes linked to resistance. Our results offer new genetic resources and markers to guide future marker-assisted breeding for PM resistance in melon.

Citing Articles

Identification of powdery mildew resistance quantitative trait loci in melon and development of resistant near-isogenic lines through marker-assisted backcrossing.

Wang C, Lin S, Huang J, Chang H, Lew D, Wang Y Bot Stud. 2024; 65(1):31.

PMID: 39495375 PMC: 11534953. DOI: 10.1186/s40529-024-00435-x.

Fine mapping and identification of candidate genes associated with powdery mildew resistance in melon ( L.).

Duan X, Yuan Y, Real N, Tang M, Ren J, Wei J Hortic Res. 2024; 11(10):uhae222.

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Possibility of genome editing for melon breeding.

Nonaka S, Ezura H Breed Sci. 2024; 74(1):47-58.

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Molecular Markers for Marker-Assisted Breeding for Biotic and Abiotic Stress in Melon ( L.): A Review.

Shahwar D, Khan Z, Park Y Int J Mol Sci. 2024; 25(12).

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References

Allen G, Flores-Vergara M, Krasynanski S, Kumar S, Thompson W . A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc. 2007; 1(5):2320-5. DOI: 10.1038/nprot.2006.384. View

Berg J, Appiano M, Santillan Martinez M, Hermans F, Vriezen W, Visser R . A transposable element insertion in the susceptibility gene CsaMLO8 results in hypocotyl resistance to powdery mildew in cucumber. BMC Plant Biol. 2015; 15:243. PMC: 4600303. DOI: 10.1186/s12870-015-0635-x. View

Breeden L, Nasmyth K . Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila. Nature. 1987; 329(6140):651-4. DOI: 10.1038/329651a0. View

Cheng H, Kun W, Liu D, Su Y, He Q . Molecular cloning and expression analysis of CmMlo1 in melon. Mol Biol Rep. 2011; 39(2):1903-7. DOI: 10.1007/s11033-011-0936-6. View

Horvath D, Stall R, Jones J, Pauly M, Vallad G, Dahlbeck D . Transgenic resistance confers effective field level control of bacterial spot disease in tomato. PLoS One. 2012; 7(8):e42036. PMC: 3411616. DOI: 10.1371/journal.pone.0042036. View

Humphry M, Consonni C, Panstruga R . mlo-based powdery mildew immunity: silver bullet or simply non-host resistance?. Mol Plant Pathol. 2010; 7(6):605-10. DOI: 10.1111/j.1364-3703.2006.00362.x. View

Iovieno P, Andolfo G, Schiavulli A, Catalano D, Ricciardi L, Frusciante L . Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp. BMC Genomics. 2015; 16:1112. PMC: 4696115. DOI: 10.1186/s12864-015-2325-3. View

Johal G, Briggs S . Reductase activity encoded by the HM1 disease resistance gene in maize. Science. 1992; 258(5084):985-7. DOI: 10.1126/science.1359642. View

Kim J, Kim W, Kwak K, Oh S, Han Y, Kang H . Glycine-rich RNA-binding proteins are functionally conserved in Arabidopsis thaliana and Oryza sativa during cold adaptation process. J Exp Bot. 2010; 61(9):2317-25. PMC: 2877889. DOI: 10.1093/jxb/erq058. View

10.

Kim H, OConnell R, Maekawa-Yoshikawa M, Uemura T, Neumann U, Schulze-Lefert P . The powdery mildew resistance protein RPW8.2 is carried on VAMP721/722 vesicles to the extrahaustorial membrane of haustorial complexes. Plant J. 2014; 79(5):835-47. DOI: 10.1111/tpj.12591. View

11.

Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N . The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009; 25(16):2078-9. PMC: 2723002. DOI: 10.1093/bioinformatics/btp352. View

12.

Orgil U, Araki H, Tangchaiburana S, Berkey R, Xiao S . Intraspecific genetic variations, fitness cost and benefit of RPW8, a disease resistance locus in Arabidopsis thaliana. Genetics. 2007; 176(4):2317-33. PMC: 1950634. DOI: 10.1534/genetics.107.070565. View

13.

Perchepied L, Bardin M, Dogimont C, Pitrat M . Relationship between Loci conferring downy mildew and powdery mildew resistance in melon assessed by quantitative trait Loci mapping. Phytopathology. 2008; 95(5):556-65. DOI: 10.1094/PHYTO-95-0556. View

14.

Perin C, Hagen S, De Conto V, Katzir N, Danin-Poleg Y, Portnoy V . A reference map of Cucumis melo based on two recombinant inbred line populations. Theor Appl Genet. 2003; 104(6-7):1017-1034. DOI: 10.1007/s00122-002-0864-x. View

15.

Pessina S, Pavan S, Catalano D, Gallotta A, Visser R, Bai Y . Characterization of the MLO gene family in Rosaceae and gene expression analysis in Malus domestica. BMC Genomics. 2014; 15:618. PMC: 4124139. DOI: 10.1186/1471-2164-15-618. View

16.

Schmidt F, Marnef A, Cheung M, Wilson I, Hancock J, Staiger D . A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8. Mol Biol Rep. 2009; 37(2):839-45. DOI: 10.1007/s11033-009-9636-x. View

17.

Takagi H, Abe A, Yoshida K, Kosugi S, Natsume S, Mitsuoka C . QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant J. 2013; 74(1):174-83. DOI: 10.1111/tpj.12105. View

18.

Zheng Y, Xu F, Li Q, Wang G, Liu N, Gong Y . QTL Mapping Combined With Bulked Segregant Analysis Identify SNP Markers Linked to Leaf Shape Traits in Using SLAF Sequencing. Front Genet. 2018; 9:615. PMC: 6290080. DOI: 10.3389/fgene.2018.00615. View