Oxidative Damage in Nile Tilapia, Oreochromis Niloticus, is Mainly Induced by Water Temperature Variation Rather Than Aurantiochytrium Sp. Meal Dietary Supplementation

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2022 Jan 4

PMID 34981327

Citations 2

Authors

Renata Oselame Nobrega

Alcir Luiz Dafre

Camila Fernandes Correa

Bruna Mattioni

Rosana Oliveira Batista

James E Pettigrew

Debora Machado Fracalossi

Affiliations

Soon will be listed here.

Abstract

We investigated whether dietary supplementation with Aurantiochytrium sp. meal, a DHA-rich source (docosahexaenoic acid, 22: 6 n-3), fed during long-term exposure to cold-suboptimal temperature (22 °C, P1), followed by short-term exposure to higher temperatures (28 °C, P2, and 33 °C, P3), would promote oxidative damage in Nile tilapia (Oreochromis niloticus). Two supplementation levels were tested: 1.0 g 100 g (D1) and 4.0 g 100 g (D4). A control diet, without the additive (D0, 0 g 100 g), and a positive control diet supplemented with cod liver oil (CLO) were also tested. The concentrations of DHA and total n-3 PUFAs in the CLO diet were similar to those found in diets D1 and D4, respectively. The parameters analyzed included hemoglobin (Hb), the antioxidant enzymes catalase, glutathione peroxidase, total glutathione, non-protein thiols, and the oxidative markers protein carbonyl and erythrocyte DNA damage. Nile tilapia did not present differences in Hb content, regardless of diet composition, but the temperature increase (P1 to P2) led to a higher Hb content. Likewise, the temperature increases promoted alterations in all antioxidant enzymes. The dietary supplementation with 1.0 g 100 g Aurantiochytrium sp. meal after P1 caused minor DNA damage in Nile tilapia, demonstrating that the additive can safely be included in winter diets, despite its high DHA concentration.

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References

AEBI H . Catalase in vitro. Methods Enzymol. 1984; 105:121-6. DOI: 10.1016/s0076-6879(84)05016-3. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe S . Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-54. PMC: 4044300. DOI: 10.1152/physrev.00040.2012. View

Chandra J, Samali A, Orrenius S . Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med. 2000; 29(3-4):323-33. DOI: 10.1016/s0891-5849(00)00302-6. View

Ellman G, Lysko H . A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem. 1979; 93(1):98-102. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Gao Y, Xie Q, Jin L, Sun B, Ji J, Chen F . Supplementation of xanthophylls decreased proinflammatory and increased anti-inflammatory cytokines in hens and chicks. Br J Nutr. 2012; 108(10):1746-55. DOI: 10.1017/S0007114512000025. View

Garrido A, Garrido F, Guerra R, Valenzuela A . Ingestion of high doses of fish oil increases the susceptibility of cellular membranes to the induction of oxidative stress. Lipids. 1989; 24(9):833-5. DOI: 10.1007/BF02544593. View

Gegotek A, Skrzydlewska E . Biological effect of protein modifications by lipid peroxidation products. Chem Phys Lipids. 2019; 221:46-52. DOI: 10.1016/j.chemphyslip.2019.03.011. View

10.

Heise K, Puntarulo S, Nikinmaa M, Abele D, Portner H . Oxidative stress during stressful heat exposure and recovery in the North Sea eelpout Zoarces viviparus L. J Exp Biol. 2006; 209(Pt 2):353-63. DOI: 10.1242/jeb.01977. View

11.

Ibrahim R, El-Houseiny W, Behairy A, Abo-Elmaaty A, Al-Sagheer A . The palliative role of Eruca sativa leaves dietary supplementation against oxidative stress, immunosuppression, and growth retardation in temperature-stressed Oreochromis niloticus. J Therm Biol. 2019; 84:26-35. DOI: 10.1016/j.jtherbio.2019.05.026. View

12.

Klein R, Borges V, Rosa C, Colares E, Robaldo R, Martinez P . Effects of increasing temperature on antioxidant defense system and oxidative stress parameters in the Antarctic fish Notothenia coriiceps and Notothenia rossii. J Therm Biol. 2017; 68(Pt A):110-118. DOI: 10.1016/j.jtherbio.2017.02.016. View

13.

Levine R, Williams J, Stadtman E, Shacter E . Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol. 1994; 233:346-57. DOI: 10.1016/s0076-6879(94)33040-9. View

14.

Liu B, Xie J, Ge X, Xu P, Wang A, He Y . Effects of anthraquinone extract from Rheum officinale Bail on the growth performance and physiological responses of Macrobrachium rosenbergii under high temperature stress. Fish Shellfish Immunol. 2010; 29(1):49-57. DOI: 10.1016/j.fsi.2010.02.018. View

15.

Lu D, Ma Q, Sun S, Zhang H, Chen L, Zhang M . Reduced oxidative stress increases acute cold stress tolerance in zebrafish. Comp Biochem Physiol A Mol Integr Physiol. 2019; 235:166-173. DOI: 10.1016/j.cbpa.2019.06.009. View

16.

Luo S, Huang Y, Xie F, Huang X, Liu Y, Wang W . Molecular cloning, characterization and expression analysis of PPAR gamma in the orange-spotted grouper (Epinephelus coioides) after the Vibrio alginolyticus challenge. Fish Shellfish Immunol. 2015; 43(2):310-24. DOI: 10.1016/j.fsi.2015.01.003. View

17.

Ma X, Qiang J, He J, Gabriel N, Xu P . Changes in the physiological parameters, fatty acid metabolism, and SCD activity and expression in juvenile GIFT tilapia (Oreochromis niloticus) reared at three different temperatures. Fish Physiol Biochem. 2015; 41(4):937-50. DOI: 10.1007/s10695-015-0059-4. View

18.

Naylor R, Hardy R, Bureau D, Chiu A, Elliott M, Farrell A . Feeding aquaculture in an era of finite resources. Proc Natl Acad Sci U S A. 2009; 106(36):15103-10. PMC: 2741212. DOI: 10.1073/pnas.0905235106. View

19.

Qiang J, Yang H, Wang H, Kpundeh M, Xu P . Interacting effects of water temperature and dietary protein level on hematological parameters in Nile tilapia juveniles, Oreochromis niloticus (L.) and mortality under Streptococcus iniae infection. Fish Shellfish Immunol. 2012; 34(1):8-16. DOI: 10.1016/j.fsi.2012.09.003. View

20.

Qiu J, Wang W, Wang L, Liu Y, Wang A . Oxidative stress, DNA damage and osmolality in the Pacific white shrimp, Litopenaeus vannamei exposed to acute low temperature stress. Comp Biochem Physiol C Toxicol Pharmacol. 2011; 154(1):36-41. DOI: 10.1016/j.cbpc.2011.02.007. View