Identification and Functional Characterization of Divergent 3'-Phosphate TRNA Ligase From

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2022 Jan 3

PMID 34976851

Citations 1

Authors

Ruofan Peng

Shigeo Yoshinari

Tetsuro Kawano-Sugaya

Ghulam Jeelani

Tomoyoshi Nozaki

Affiliations

Soon will be listed here.

Abstract

HSPC117/RtcB, 3'-phosphate tRNA ligase, is a critical enzyme involved in tRNA splicing and maturation. HSPC117/RtcB is also involved in mRNA splicing of some protein-coding genes including . , a protozoan parasite responsible for human amebiasis, possesses two RtcB proteins (EhRtcB1 and 2), but their biological functions remain unknown. Both RtcBs show kinship with mammalian/archaeal type, and all amino acid residues present in the active sites are highly conserved, as suggested by protein alignment and phylogenetic analyses. EhRtcB1 was demonstrated to be localized to the nucleus, while EhRtcB2 was in the cytosol. EhRtcB1, but not EhRtcB2, was required for optimal growth of trophozoites. Both EhRtcB1 (in cooperation with EhArchease) and EhRtcB2 showed RNA ligation activity . The predominant role of EhRtcB1 in tRNA(UAU) processing was demonstrated in EhRtcB1- and 2-gene silenced strains. Taken together, we have demonstrated the conservation of tRNA splicing and functional diversification of RtcBs in this amoebozoan lineage.

Citing Articles

Transfer RNA-Derived Small RNAs in the Pathogenesis of Parasitic Protozoa.

Peng R, Santos H, Nozaki T Genes (Basel). 2022; 13(2).

PMID: 35205331 PMC: 8872473. DOI: 10.3390/genes13020286.

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