Description of a Recovery Model in Rabbits for the Study of the Late Phase of Liver Ischemia-Reperfusion Injury

Overview

Journal In Vivo

Specialty Oncology

Date 2022 Jan 1

PMID 34972710

Citations 1

Authors

Athina G Mantelou

Argyro Zacharioudaki

George Pappas-Gogos

Apostolos Papalois

Alexandra Papoudou-Bai

Anna Goussia

Georgios K Glantzounis

Affiliations

Soon will be listed here.

Abstract

Aim: Description of an anesthetic recovery model with endotracheal intubation in rabbits which provides metabolic stability for the study of the late phase of liver ischemia/reperfusion (I/R) injury.

Materials And Methods: Two groups of New Zealand rabbits, n=7 in each, were used: Ischemia/reperfusion (I/R) group (45 min of partial liver ischemia/reperfusion) and no intervention (sham) group. Blood alanine aminotransferase, lactate, pH values, mean arterial pressure and pCO were calculated at baseline, and at 2 and 24 h post reperfusion. Tissue samples from left (ischemic) and right (non-ischemic) liver lobes were examined at 2 and 24 h after reperfusion.

Results: The I/R group presented significantly higher levels of alanine aminotransferase (p=0.001) at 2 and 24 h, and of lactate (p=0.016) at 2 h post reperfusion. No differences were documented for pH, mean arterial pressure and pCO Histological exanimation revealed significant injury at 24 h post reperfusion for the I/R group.

Conclusion: This anesthetic recovery model permitted avoidance of hypoxia and respiratory acidosis, allowing the study of the late phase of I/R injury.

Citing Articles

Multi-time point transcriptomics and metabolomics reveal key transcription and metabolic features of hepatic ischemia-reperfusion injury in mice.

Li Q, Qin X, Wang L, Hu D, Liao R, Yu H Genes Dis. 2025; 12(2):101465.

PMID: 39759115 PMC: 11697123. DOI: 10.1016/j.gendis.2024.101465.

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