Detection of Early Myocardial Cell Death in Owl Monkeys (Aotus Nancymai) Using Complement Component C9 Immunohistochemistry in Formalin-fixed Paraffin-embedded Heart Tissues: A Retrospective Study

Overview

Journal J Med Primatol

Publisher Wiley

Date 2021 Dec 31

PMID 34971004

Citations 2

Authors

Alfonso S Gozalo

Lynn E Lambert

Patricia M Zerfas

William R Elkins

Affiliations

Soon will be listed here.

Abstract

Background: Owl monkeys are commonly used in biomedical research which is affected by the high incidence of cardiomyopathy in this species. Occasionally, owl monkeys with no clinical signs of heart disease are found dead and at necropsy show no, or very mild, cardiomyopathy. A possible explanation for sudden death is acute myocardial infarction; however, early myocardial changes may be difficult to assess by conventional stains and light microscopy.

Methods: Complement component C9 immunohistochemistry was performed in paraffin-embedded heart tissue samples from owl monkeys who died suddenly, or were euthanized due to sickness, to determine whether these animals suffered from acute myocardial infarcts.

Results And Conclusion: C9 deposits were found in the myocardium of 19 out of 20 (95%) animals. The findings in this study suggest owl monkeys suffer from acute myocardial infarcts, and complement component C9 immunohistochemistry may be a useful diagnostic tool.

Citing Articles

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References

Liaudet L, Calderari B, Pacher P . Pathophysiological mechanisms of catecholamine and cocaine-mediated cardiotoxicity. Heart Fail Rev. 2014; 19(6):815-24. DOI: 10.1007/s10741-014-9418-y. View

Slavich M, Patel R . Coronary artery spasm: Current knowledge and residual uncertainties. Int J Cardiol Heart Vasc. 2017; 10:47-53. PMC: 5462634. DOI: 10.1016/j.ijcha.2016.01.003. View

Gozalo A, Elkins W, Lambert L . Eosinophilic aortitis with thoracic aortic aneurysm and rupture in a captive-born owl monkey. J Med Primatol. 2018; 47(6):423-426. PMC: 11025315. DOI: 10.1111/jmp.12373. View

Fishbein M, Maclean D, Maroko P . The histopathologic evolution of myocardial infarction. Chest. 1978; 73(6):843-9. DOI: 10.1378/chest.73.6.843. View

Stamboul K, Fauchier L, Gudjoncik A, Buffet P, Garnier F, Lorgis L . New insights into symptomatic or silent atrial fibrillation complicating acute myocardial infarction. Arch Cardiovasc Dis. 2015; 108(11):598-605. DOI: 10.1016/j.acvd.2015.06.009. View

Vakeva A, Morgan B, Tikkanen I, Helin K, Laurila P, Meri S . Time course of complement activation and inhibitor expression after ischemic injury of rat myocardium. Am J Pathol. 1994; 144(6):1357-68. PMC: 1887457. View

HAFT J . Cardiovascular injury induced by sympathetic catecholamines. Prog Cardiovasc Dis. 1974; 17(1):73-86. DOI: 10.1016/0033-0620(74)90039-5. View

NACHLAS M, Shnitka T . Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity. Am J Pathol. 1963; 42:379-405. PMC: 1949652. View

Chapman Jr W, Crowell W, Isaac W . Spontaneous lesions seen at necropsy in 7 owl monkeys (Aotus trivirgatus). Lab Anim Sci. 1973; 23(3):434-42. View

10.

Knowlen G, Weller R, Perry R, Baer J, Gozalo A . Hypertrophic cardiomyopathy in owl monkeys (Aotus spp.). Comp Med. 2013; 63(3):279-87. PMC: 3690434. View

11.

Baer J, Gibson S, Weller R, Buschbom R, Leathers C . Naturally occurring aortic aneurysms in owl monkeys (Aotus spp.). Lab Anim Sci. 1992; 42(5):463-6. View

12.

Gozalo A, Elkins W, Lambert L, Stock F, Thomas 3rd M, Woodward R . Genetic diversity of Klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony. J Med Primatol. 2016; 45(6):312-317. PMC: 5288377. DOI: 10.1111/jmp.12229. View

13.

Rajendra R, Brady A, Parks V, Massey C, Gibson S, Abee C . The normal and abnormal owl monkey (Aotus sp.) heart: looking at cardiomyopathy changes with echocardiography and electrocardiography. J Med Primatol. 2010; 39(3):143-50. PMC: 6088386. DOI: 10.1111/j.1600-0684.2010.00403.x. View

14.

Jasra S, Badian C, Macri I, Ra P . Recognition of early myocardial infarction by immunohistochemical staining with cardiac troponin-I and complement C9. J Forensic Sci. 2012; 57(6):1595-600. DOI: 10.1111/j.1556-4029.2012.02172.x. View

15.

Turillazzi E, DI Paolo M, Neri M, Riezzo I, Fineschi V . A theoretical timeline for myocardial infarction: immunohistochemical evaluation and western blot quantification for Interleukin-15 and Monocyte chemotactic protein-1 as very early markers. J Transl Med. 2014; 12:188. PMC: 4094437. DOI: 10.1186/1479-5876-12-188. View

16.

Laurent S, Boutouyrie P . The structural factor of hypertension: large and small artery alterations. Circ Res. 2015; 116(6):1007-21. DOI: 10.1161/CIRCRESAHA.116.303596. View

17.

Mathey D, Schofer J, Schafer H, Hamdoch T, Joachim H, Ritgen A . Early accumulation of the terminal complement-complex in the ischaemic myocardium after reperfusion. Eur Heart J. 1994; 15(3):418-23. DOI: 10.1093/oxfordjournals.eurheartj.a060516. View

18.

Zehender M, Faber T, Koscheck U, Meinertz T, Just H . Ventricular tachyarrhythmias, myocardial ischemia, and sudden cardiac death in patients with hypertensive heart disease. Clin Cardiol. 1995; 18(7):377-83. DOI: 10.1002/clc.4960180705. View

19.

Sabatasso S, Mangin P, Fracasso T, Moretti M, Docquier M, Djonov V . Early markers for myocardial ischemia and sudden cardiac death. Int J Legal Med. 2016; 130(5):1265-80. DOI: 10.1007/s00414-016-1401-9. View

20.

Sumitra M, Manikandan P, Nayeem M, Manohar B, Lokanadam B, Vairamuthu S . Time course studies on the initiation of complement activation in acute myocardial infarction induced by coronary artery ligation in rats. Mol Cell Biochem. 2005; 268(1-2):149-58. DOI: 10.1007/s11010-005-3856-8. View