Prevalence of Merkel Cell Polyomavirus (MCPyV) in the Oral Cavity Biopsies in Northern Iran

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2021 Dec 30

PMID 34967573

Citations 3

Authors

Mina Hasani Estalkhi

Maryam Seyed Majidi

Farzin Sadeghi

Mohammad Chehrazi

Arghavan Zebardast

Ali Hasanzadeh

Yousef Yahyapour

Affiliations

Soon will be listed here.

Abstract

Objective: Infection with human tumor viruses is one of the hypothesized causes of cancer. The current investigation aimed to explore the presence and quantitative analysis of a new human tumor virus, Merkel cell polyomavirus (MCPyV) in tissue samples of 114 patients with oral cavity lesions including oral squamous cell carcinoma (OSCC), oral lichen planus (OLP), Dysplasia and oral irritation fibroma (OIF) in Northern Iran.

Methods: From 114 formalin fixed paraffin embedded samples; 35 with SCC, 29 with OLP, 14 with dysplasia and 36 with OIF were cut, deparaffinized and DNA was extracted. Quantitative detection of MCPyV large T antigen was performed by absolute quantitative Real-Time PCR.

Result: MCPyV DNA was detected in 30.6% (n: 11/36) of IF, 24.1% (n; 7/29) of OLP, 21.4% (n:3/14) of dysplasia and 20% (n;7/35) of OSCC samples. The mean MCPyV DNA copy number was 2.32×10-2 ± 3.97 ×10-2, 2.02×10-2 (SD=3.13×10-2), 2.69×10-4 (SD=2.51×10-4), and 2.56×10-4 (SD=6.73×10-4) per cell in OSCC, dysplasia and both of OLP and OIF samples, respectively (P=0.76).

Conclusion: This study provides the first data from Iran regarding the presence of MCPyV genome in oral cavity lesions and oral cancer. These results also emphasize that MCPyV has an active role in the occurrence of oral lesions and progression to cancer. Further studies should be carried out to clarify the role of MCPyV in oral cavity lesions.

Citing Articles

The Role of Oncogenic Viruses in Head and Neck Cancers: Epidemiology, Pathogenesis, and Advancements in Detection Methods.

Samara P, Athanasopoulos M, Mastronikolis S, Kyrodimos E, Athanasopoulos I, Mastronikolis N Microorganisms. 2024; 12(7).

PMID: 39065250 PMC: 11279059. DOI: 10.3390/microorganisms12071482.

Merkel Cell Polyomavirus in the Context of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders.

Passerini S, Babini G, Merenda E, Carletti R, Scribano D, Rosa L Biomedicines. 2024; 12(4).

PMID: 38672065 PMC: 11047982. DOI: 10.3390/biomedicines12040709.

Prevalence of Co-infection by Human Papillomavirus, Epstein- Barr Virus and Merkel Cell Polyomavirus in Iranian Oral Cavity Cancer and Pre-malignant Lesions.

Saber Amoli S, Hasanzadeh A, Sadeghi F, Chehrazi M, Seyedmajidi M, Zebardast A Int J Mol Cell Med. 2022; 11(1):64-77.

PMID: 36397808 PMC: 9653548. DOI: 10.22088/IJMCM.BUMS.11.1.64.

References

Loyo M, Guerrero-Preston R, Brait M, Hoque M, Chuang A, Kim M . Quantitative detection of Merkel cell virus in human tissues and possible mode of transmission. Int J Cancer. 2009; 126(12):2991-6. PMC: 2856748. DOI: 10.1002/ijc.24737. View

Pezzuto F, Buonaguro L, Caponigro F, Ionna F, Starita N, Annunziata C . Update on Head and Neck Cancer: Current Knowledge on Epidemiology, Risk Factors, Molecular Features and Novel Therapies. Oncology. 2015; 89(3):125-36. DOI: 10.1159/000381717. View

Warnakulasuriya S . Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 2008; 45(4-5):309-16. DOI: 10.1016/j.oraloncology.2008.06.002. View

Zheng Y, Xia P, Zheng H, Takahashi H, Masuda S, Takano Y . The screening of viral risk factors in tongue and pharyngolaryngeal squamous carcinoma. Anticancer Res. 2010; 30(4):1233-8. View

Dhull A, Atri R, Dhankhar R, Chauhan A, Kaushal V . Major Risk Factors in Head and Neck Cancer: A Retrospective Analysis of 12-Year Experiences. World J Oncol. 2018; 9(3):80-84. PMC: 6031231. DOI: 10.14740/wjon1104w. View

Feng H, Shuda M, Chang Y, Moore P . Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science. 2008; 319(5866):1096-100. PMC: 2740911. DOI: 10.1126/science.1152586. View

Salehi-Vaziri M, Sadeghi F, Alamsi-Hashiani A, Haeri H, Monavari S, Keyvani H . Merkel cell polyomavirus and human papillomavirus infections in cervical disease in Iranian women. Arch Virol. 2015; 160(5):1181-7. DOI: 10.1007/s00705-015-2368-4. View

Bagan J, Sarrion G, Jimenez Y . Oral cancer: clinical features. Oral Oncol. 2010; 46(6):414-7. DOI: 10.1016/j.oraloncology.2010.03.009. View

Tanio S, Matsushita M, Kuwamoto S, Horie Y, Kodani I, Murakami I . Low prevalence of Merkel cell polyomavirus with low viral loads in oral and maxillofacial tumours or tumour-like lesions from immunocompetent patients: Absence of Merkel cell polyomavirus-associated neoplasms. Mol Clin Oncol. 2016; 3(6):1301-1306. PMC: 4665147. DOI: 10.3892/mco.2015.629. View

10.

Mohebbi E, Noormohamadi Z, Sadeghi-Rad H, Sadeghi F, Yahyapour Y, Vaziri F . Low viral load of Merkel cell polyomavirus in Iranian patients with head and neck squamous cell carcinoma: Is it clinically important?. J Med Virol. 2017; 90(2):344-350. DOI: 10.1002/jmv.24953. View

11.

Windon M, Fakhry C, Rooper L, Ha P, Schoppy D, Miles B . The Role of Age and Merkel Cell Polyomavirus in Oral Cavity Cancers. Otolaryngol Head Neck Surg. 2020; 163(6):1194-1197. PMC: 8067664. DOI: 10.1177/0194599820932553. View

12.

Kantola K, Sadeghi M, Lahtinen A, Koskenvuo M, Aaltonen L, Mottonen M . Merkel cell polyomavirus DNA in tumor-free tonsillar tissues and upper respiratory tract samples: implications for respiratory transmission and latency. J Clin Virol. 2009; 45(4):292-5. PMC: 7172143. DOI: 10.1016/j.jcv.2009.04.008. View

13.

Bello I, Soini Y, Salo T . Prognostic evaluation of oral tongue cancer: means, markers and perspectives (II). Oral Oncol. 2010; 46(9):636-43. DOI: 10.1016/j.oraloncology.2010.06.008. View

14.

Harmse J, Carey F, Baird A, Craig S, Christie K, Hopwood D . Merkel cells in the human oesophagus. J Pathol. 1999; 189(2):176-9. DOI: 10.1002/(SICI)1096-9896(199910)189:2<176::AID-PATH416>3.0.CO;2-U. View

15.

Yahyapour Y, Shamsi-Shahrabadi M, Mahmoudi M, Siadati S, Shahryar S, Shokri-Shirvani J . Evaluation of human papilloma virus infection in patients with esophageal squamous cell carcinoma from the Caspian Sea area, north of Iran. Asian Pac J Cancer Prev. 2012; 13(4):1261-6. DOI: 10.7314/apjcp.2012.13.4.1261. View

16.

Sadeghi F, Salehi-Vaziri M, Alizadeh A, Ghodsi S, Bokharaei-Salim F, Fateh A . Detection of Merkel cell polyomavirus large T-antigen sequences in human central nervous system tumors. J Med Virol. 2015; 87(7):1241-7. DOI: 10.1002/jmv.24178. View

17.

Regnault M, Vigarios E, Projetti F, Herbault-Barres B, Tournier E, Lamant L . No detection of Merkel cell polyomavirus in oral lichen planus: Results of a preliminary study in a French cohort of patients. J Med Virol. 2017; 89(11):2055-2057. DOI: 10.1002/jmv.24887. View

18.

Behdarvand A, Zamani M, Sadeghi F, Yahyapour Y, Vaziri F, Rahimi Jamnani F . Evaluation of Merkel cell polyomavirus in non-small cell lung cancer and adjacent normal cells. Microb Pathog. 2017; 108:21-26. DOI: 10.1016/j.micpath.2017.04.033. View

19.

Moens U, Van Ghelue M, Ehlers B . Are human polyomaviruses co-factors for cancers induced by other oncoviruses?. Rev Med Virol. 2014; 24(5):343-60. DOI: 10.1002/rmv.1798. View

20.

Yahyapour Y, Sadeghi F, Alizadeh A, Rajabnia R, Siadati S . Detection of Merkel Cell Polyomavirus and Human Papillomavirus in Esophageal Squamous Cell Carcinomas and Non-Cancerous Esophageal Samples in Northern Iran. Pathol Oncol Res. 2016; 22(4):667-72. DOI: 10.1007/s12253-016-0048-7. View