Genome-Wide Identification and Characterization of GhCOMT Gene Family During Fiber Development and Verticillium Wilt Resistance in Cotton

Overview

Journal Plants (Basel)

Date 2021 Dec 28

PMID 34961226

Citations 3

Authors

Cuicui Wu

Dongyun Zuo

Shuiping Xiao

Qiaolian Wang

Hailiang Cheng

Limin Lv

Youping Zhang

Pengbo Li

Guoli Song

Affiliations

Soon will be listed here.

Abstract

Caffeic acid -methyltransferases (COMTs) play an essential role in lignin synthesis procession, especially in the plant's phenylalanine metabolic pathway. The content of COMT genes in cotton and the relationship between their expression patterns have not been studied clearly in cotton. In this study, we have identified 190 COMT genes in cotton, which were classified into three groups (I, II and III), and mapped on the cotton chromosomes. In addition, we found that 135 of the 190 COMT genes result from dispersed duplication (DSD) and whole-genome duplication (WGD), indicating that DSD and WGD were the main forces driving COMT gene expansion. The Ka/Ks analysis showed that and evolved from and through positive selection. The results of qRT-PCR showed that , , and were related to lignin content during the cotton fiber development. , , , and responded to Verticillium Wilt (VW) and maybe related to VW resistance through lignin synthesis. Conclusively, this study found that GhCOMTs were highly expressed in the secondary wall thickening stage and VW. These results provide a clue for studying the functions of GhCOMTs in the development of cotton fiber and VW resistance and could lay a foundation for breeding cotton cultivates with higher quantity and high resistance to VW.

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