Effects of the Washing Time and Washing Solution on the Biocompatibility and Mechanical Properties of 3D Printed Dental Resin Materials

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2021 Dec 28

PMID 34960960

Citations 18

Authors

Na-Kyung Hwangbo

Na-Eun Nam

Jong-Hoon Choi

Jong-Eun Kim

Affiliations

Soon will be listed here.

Abstract

Three-dimensional (3D) printing technology is highly regarded in the field of dentistry. Three-dimensional printed resin restorations must undergo a washing process to remove residual resin on the surface after they have been manufactured. However, the effect of the use of different washing solutions and washing times on the biocompatibility of the resulting resin restorations is unclear. Therefore, we prepared 3D-printed denture teeth and crown and bridge resin, and then washed them with two washing solutions (isopropyl alcohol and tripropylene glycol monomethyl ether) using different time points (3, 5, 10, 15, 30, 60, and 90 min). After this, the cell viability, cytotoxicity, and status of human gingival fibroblasts were evaluated using confocal laser scanning. We also analyzed the flexural strength, flexural modulus, and surface SEM imaging. Increasing the washing time increased the cell viability and decreased the cytotoxicity ( < 0.001). Confocal laser scanning showed distinct differences in the morphology and number of fibroblasts. Increasing the washing time did not significantly affect the flexural strength and surface, but the flexural modulus of the 90 min washing group was 1.01 ± 0.21 GPa (mean ± standard deviation), which was lower than that of all the other groups and decreased as the washing time increased. This study confirmed that the washing time affected the biocompatibility and mechanical properties of 3D printed dental resins.

Citing Articles

Flexural Strength, Fatigue Behavior, and Microhardness of Three-Dimensional (3D)-Printed Resin Material for Indirect Restorations: A Systematic Review.

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Evaluation of Internal and Marginal Accuracy (Trueness and Precision) of Laminates Using DLP Printing and Milling Methods.

Noh M, Lee H, Lee W, Kim J, Kim J Biomimetics (Basel). 2025; 10(1).

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Polymeric Materials Used in 3DP in Dentistry-Biocompatibility Testing Challenges.

Rus F, Neculau C, Imre M, Duica F, Popa A, Moisa R Polymers (Basel). 2025; 16(24.

PMID: 39771402 PMC: 11679966. DOI: 10.3390/polym16243550.

Wear Behavior and Water Sorption of Additively Manufactured Resin-Based Splint Materials.

Wulff J, Merle C, Hahnel S, Rosentritt M Materials (Basel). 2024; 17(23).

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A Novel 3-Dimensional Printed Nanoceramic Hybrid Resin Fixed Lingual Retainer: Characterization and Mechanical Tests.

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