Pea Proteins Have Anabolic Effects Comparable to Milk Proteins on Whole Body Protein Retention and Muscle Protein Metabolism in Old Rats

Overview

Journal Nutrients

Date 2021 Dec 28

PMID 34959786

Citations 7

Authors

Jerome Salles

Christelle Guillet

Olivier le Bacquer

Carmen Malnero-Fernandez

Christophe Giraudet

Veronique Patrac

Alexandre Berry

Philippe Denis

Corinne Pouyet

Marine Gueugneau

Yves Boirie

Heidi Jacobs

Stephane Walrand

Affiliations

Soon will be listed here.

Abstract

Plant proteins are attracting rising interest due to their pro-health benefits and environmental sustainability. However, little is known about the nutritional value of pea proteins when consumed by older people. Herein, we evaluated the digestibility and nutritional efficiency of pea proteins compared to casein and whey proteins in old rats. Thirty 20-month-old male Wistar rats were assigned to an isoproteic and isocaloric diet containing either casein (CAS), soluble milk protein (WHEY) or Pisane™ pea protein isolate for 16 weeks. The three proteins had a similar effect on nitrogen balance, true digestibility and net protein utilization in old rats, which means that different protein sources did not alter body composition, tissue weight, skeletal muscle protein synthesis or degradation. Muscle mitochondrial activity, inflammation status and insulin resistance were similar between the three groups. In conclusion, old rats used pea protein with the same efficiency as casein or whey proteins, due to its high digestibility and amino acid composition. Using these plant-based proteins could help older people diversify their protein sources and more easily achieve nutritional intake recommendations.

Citing Articles

Pea and soy fortified with leucine stimulates muscle protein synthesis comparable to whey in a murine ageing model.

Dijk F, van Dijk M, Roberts J, van Helvoort A, Furber M Eur J Nutr. 2024; 64(1):12.

PMID: 39567431 PMC: 11579064. DOI: 10.1007/s00394-024-03506-8.

Circulating Amino Acid Concentration after the Consumption of Pea or Whey Proteins in Young and Older Adults Affects Protein Synthesis in C2C12 Myotubes.

Salles J, Gueugneau M, Laleg K, Giraudet C, Sanchez P, Blot A Nutrients. 2024; 16(17).

PMID: 39275186 PMC: 11397729. DOI: 10.3390/nu16172870.

Fortification of Pea and Potato Protein Isolates in Oat-Based Milk Alternatives; Effects on the Sensory and Volatile Profile.

McCarron R, Methven L, Grahl S, Elliott R, Lignou S Foods. 2024; 13(13).

PMID: 38998581 PMC: 11241158. DOI: 10.3390/foods13132075.

Common questions and misconceptions about protein supplementation: what does the scientific evidence really show?.

Antonio J, Evans C, Ferrando A, Stout J, Antonio B, Cinteo H J Int Soc Sports Nutr. 2024; 21(1):2341903.

PMID: 38626029 PMC: 11022925. DOI: 10.1080/15502783.2024.2341903.

A multi-center prospective study of plant-based nutritional support in adult community-based patients at risk of disease-related malnutrition.

Delsoglio M, Griffen C, Syed R, Cookson T, Saliba H, Vowles A Front Nutr. 2023; 10:1297624.

PMID: 38024371 PMC: 10667471. DOI: 10.3389/fnut.2023.1297624.

References

Guillet C, Boirie Y . Insulin resistance: a contributing factor to age-related muscle mass loss?. Diabetes Metab. 2006; 31 Spec No 2:5S20-5S26. DOI: 10.1016/s1262-3636(05)73648-x. View

Rondanelli M, Nichetti M, Peroni G, Faliva M, Naso M, Gasparri C . Where to Find Leucine in Food and How to Feed Elderly With Sarcopenia in Order to Counteract Loss of Muscle Mass: Practical Advice. Front Nutr. 2021; 7:622391. PMC: 7874106. DOI: 10.3389/fnut.2020.622391. View

Banaszek A, Townsend J, Bender D, Vantrease W, Marshall A, Johnson K . The Effects of Whey vs. Pea Protein on Physical Adaptations Following 8-Weeks of High-Intensity Functional Training (HIFT): A Pilot Study. Sports (Basel). 2019; 7(1). PMC: 6358922. DOI: 10.3390/sports7010012. View

Salles J, Chanet A, Berry A, Giraudet C, Patrac V, Domingues-Faria C . Fast digestive, leucine-rich, soluble milk proteins improve muscle protein anabolism, and mitochondrial function in undernourished old rats. Mol Nutr Food Res. 2017; 61(11). DOI: 10.1002/mnfr.201700287. View

van Vliet S, Burd N, van Loon L . The Skeletal Muscle Anabolic Response to Plant- versus Animal-Based Protein Consumption. J Nutr. 2015; 145(9):1981-91. DOI: 10.3945/jn.114.204305. View

Zhao Y, Cholewa J, Shang H, Yang Y, Ding X, Wang Q . Advances in the Role of Leucine-Sensing in the Regulation of Protein Synthesis in Aging Skeletal Muscle. Front Cell Dev Biol. 2021; 9:646482. PMC: 8047301. DOI: 10.3389/fcell.2021.646482. View

Pirman T, Combe E, Mirand P, Stekar J, Oresnik A . Differential effects of cooked common bean (Phaseolus vulgaris) and lentil (Lens esculenta puyensis) feeding on protein and nucleic acid contents in intestines, liver and muscles in rats. Ann Nutr Metab. 2004; 48(4):281-7. DOI: 10.1159/000080521. View

Floret C, Monnet A, Micard V, Walrand S, Michon C . Replacement of animal proteins in food: How to take advantage of nutritional and gelling properties of alternative protein sources. Crit Rev Food Sci Nutr. 2021; 63(7):920-946. DOI: 10.1080/10408398.2021.1956426. View

Gutierrez Cortes N, Pertuiset C, Dumon E, Borlin M, Hebert-Chatelain E, Pierron D . Novel mitochondrial DNA mutations responsible for maternally inherited nonsyndromic hearing loss. Hum Mutat. 2012; 33(4):681-9. DOI: 10.1002/humu.22023. View

10.

. Protein and amino acid requirements in human nutrition. World Health Organ Tech Rep Ser. 2008; (935):1-265, back cover. View

11.

Berrazaga I, Micard V, Gueugneau M, Walrand S . The Role of the Anabolic Properties of Plant- versus Animal-Based Protein Sources in Supporting Muscle Mass Maintenance: A Critical Review. Nutrients. 2019; 11(8). PMC: 6723444. DOI: 10.3390/nu11081825. View

12.

Guillin F, Gaudichon C, Guerin-Deremaux L, Lefranc-Millot C, Azzout-Marniche D, Khodorova N . Multi-criteria assessment of pea protein quality in rats: a comparison between casein, gluten and pea protein alone or supplemented with methionine. Br J Nutr. 2020; 125(4):389-397. DOI: 10.1017/S0007114520002883. View

13.

Zhu Y, Zhang H, Wei Y, Cai M, Gu R, Wang Y . Pea-derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS-1/PI3K/AKT and blocking ROS-mediated p38MAPK signaling. J Food Biochem. 2020; 44(11):e13454. DOI: 10.1111/jfbc.13454. View

14.

Pirman T, Combe E, Ribeyre M, Prugnaud J, Stekar J, Mirand P . Differential effects of cooked beans and cooked lentils on protein metabolism in intestine and muscle in growing rats. Ann Nutr Metab. 2006; 50(3):197-205. DOI: 10.1159/000090741. View

15.

Pereira M, Silva M, da Cunha F, Moriscot A, Aoki M, Miyabara E . Leucine supplementation improves regeneration of skeletal muscles from old rats. Exp Gerontol. 2015; 72:269-77. DOI: 10.1016/j.exger.2015.10.006. View

16.

Lohrke B, Saggau E, Schadereit R, Beyer M, Bellmann O, Kuhla S . Activation of skeletal muscle protein breakdown following consumption of soyabean protein in pigs. Br J Nutr. 2001; 85(4):447-57. DOI: 10.1079/bjn2000291. View

17.

Laleg K, Salles J, Berry A, Giraudet C, Patrac V, Guillet C . Nutritional evaluation of mixed wheat-faba bean pasta in growing rats: impact of protein source and drying temperature on protein digestibility and retention. Br J Nutr. 2018; 121(5):496-507. DOI: 10.1017/S0007114518003586. View

18.

Sugawara K, Takahashi H, Kashiwagura T, Yamada K, Yanagida S, Homma M . Effect of anti-inflammatory supplementation with whey peptide and exercise therapy in patients with COPD. Respir Med. 2012; 106(11):1526-34. DOI: 10.1016/j.rmed.2012.07.001. View

19.

Overduin J, Guerin-Deremaux L, Wils D, Lambers T . NUTRALYS(®) pea protein: characterization of in vitro gastric digestion and in vivo gastrointestinal peptide responses relevant to satiety. Food Nutr Res. 2015; 59:25622. PMC: 4400298. DOI: 10.3402/fnr.v59.25622. View

20.

Combe E, Pirman T, Stekar J, Houlier M, Mirand P . Differential effect of lentil feeding on proteosynthesis rates in the large intestine, liver and muscle of rats. J Nutr Biochem. 2004; 15(1):12-7. DOI: 10.1016/j.jnutbio.2003.08.006. View