Modulation of Phase Separation by RNA: A Glimpse on N-Methyladenosine Modification

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2021 Dec 27

PMID 34957114

Citations 11

Authors

Yingfeng Su

Yasen Maimaitiyiming

Lingfang Wang

Xiaodong Cheng

Chih-Hung Hsu

Affiliations

Soon will be listed here.

Abstract

Phase separation is the driving force behind formation of various biomolecular condensates (BioMCs), which sub-compartmentalize certain cellular components in a membraneless manner to orchestrate numerous biological processes. Many BioMCs are composed of proteins and RNAs. While the features and functions of proteins are well studied, less attention was paid to the other essential component RNAs. Here, we describe how RNA contributes to the biogenesis, dissolution, and properties of BioMCs as a multivalence providing scaffold for proteins/RNA to undergo phase separation. Specifically, we focus on N-methyladenosine (mA), the most widely distributed dynamic post-transcriptional modification, which would change the charge, conformation, and RNA-binding protein (RBP) anchoring of modified RNA. mA RNA-modulated phase separation is a new perspective to illustrate mA-mediated various biological processes. We summarize mA main functions as "beacon" to recruit reader proteins and "structural switcher" to alter RNA-protein and RNA-RNA interactions to modulate phase separation and regulate the related biological processes.

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